LOCUS       sequence-422106-       10572 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 Spike_Delta in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10572)
  TITLE     Host-SARS-CoV2 protein interactions reveal viral disease mechanisms 
            and antiviral innate defense
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLD-puro-Cc-SARS-CoV-2_Spike_Delta-VA
FEATURES             Location/Qualifiers
     source          1..10572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     761..781
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1070..1273
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1224..1244
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1270..1294
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1325..1349
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4188..4212)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4292..4315
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4352..4375
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4388..4405
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4415..4435
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4448..4468
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4481..4546
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4594..4711
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4760..5270
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5189..5207
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5292..5891
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5292..5311)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5788..5808
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6019..6252
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6324..6445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6361..6380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6415..6434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6485..6620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6640..6659)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6641..6659)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6669..6686)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6669..6685)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6678..6700)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6827..7282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6914..6933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7124..7145
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7308..7412
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7413..7481,7482..8273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7631..7650)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8444..9032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8933..8952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9186..9203
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9320..9341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9356..9361,9362..9379,9380..9386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9394..9410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9399..9421
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9418..9434
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9418..9434
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9453..9473
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9455..9473
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9501..9727
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9728..9908
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9955..10080
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat
      781 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
      841 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      901 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      961 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     1021 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     1081 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1141 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1201 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1261 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgtac
     1321 gcgtacaagt ttgtacaaaa aagttggcat gttcgtgttc ctggtgctgc tgccactggt
     1381 gagctcccag tgcgtgaacc tgcggaccag aacccagctg ccacctgcct acaccaactc
     1441 cttcaccagg ggcgtgtact acccagacaa ggtgttcaga tccagcgtgc tgcactctac
     1501 ccaggacctg ttcctgccat tcttctctaa cgtgacctgg ttccacgcca tccacgtgtc
     1561 tggaaccaac ggcaccaaga gattcgacaa cccagtgctg ccattcaacg acggcgtgta
     1621 cttcgcttct atcgagaagt ctaacatcat caggggttgg atcttcggaa ccaccctgga
     1681 ctctaagacc cagtctctgc tgatcgtgaa caacgctacc aacgtggtga tcaaggtgtg
     1741 cgagttccag ttctgcaacg acccattcct ggacgtgtac taccacaaga acaacaagtc
     1801 ttggatggaa agcggcgtgt acagctctgc taacaactgc accttcgagt acgtgtctca
     1861 gcctttcctg atggacctgg aaggaaagca gggaaacttc aagaacctga gagagttcgt
     1921 gttcaagaac atcgacggtt acttcaagat atacagcaag cacaccccaa tcaacctggt
     1981 gagggacctg ccacagggtt tctctgctct ggagcccctg gtggacctgc caatcggcat
     2041 caacatcacc aggttccaga ccctgctggc tctgcacagg tcttacctga ccccaggcga
     2101 cagcagctct ggatggaccg ccggcgccgc tgcttactac gtgggatacc tgcagccaag
     2161 gaccttcctg ctgaagtaca acgaaaacgg caccatcacc gacgccgtgg actgcgctct
     2221 ggacccactg tctgagacca agtgcaccct gaagtctttc accgtggaaa agggaatcta
     2281 ccagaccagc aacttccgcg tgcagcccac cgagtctatc gtgagattcc caaacatcac
     2341 caacctgtgc ccattcggag aagtgttcaa cgctaccagg ttcgccagcg tgtacgcttg
     2401 gaacaggaag agaatcagca actgcgtggc cgactactct gtgctgtaca actctgcctc
     2461 cttctccacc ttcaagtgct acggcgtgtc cccaaccaag ctgaacgacc tgtgcttcac
     2521 caacgtgtac gctgactcct tcgtgatcag aggagacgaa gtgagacaga tcgctcccgg
     2581 tcagaccgga aagatcgccg actacaacta caagctgcca gacgacttca ccggttgcgt
     2641 gatcgcttgg aactctaaca acctggactc taaggtggga ggcaactaca actaccggta
     2701 caggctgttc cgcaagagca acctgaagcc tttcgaaagg gacatcagca ccgaaatcta
     2761 ccaggctgga tctaagccat gcaacggagt ggaaggattc aactgctact tccctctgca
     2821 gtcttacgga ttccagccaa ccaacggagt gggataccag ccatacagag tggtggtgct
     2881 gtctttcgag ctgctgcacg ctcctgctac cgtgtgcgga ccaaagaagt ctaccaacct
     2941 ggtgaagaac aagtgcgtga acttcaactt caacggactg accggaaccg gagtgctgac
     3001 cgaatccaac aagaagttcc tgcctttcca gcagttcggt agagacatcg ccgacaccac
     3061 cgacgctgtg agagacccac agaccctgga aatcctggac atcaccccct gctctttcgg
     3121 aggagtgagc gtgatcaccc caggaaccaa cacctccaac caggtggccg tgctgtacca
     3181 gggcgtgaac tgcaccgaag tgccagtggc catccacgct gaccagctga ccccaacctg
     3241 gagagtgtac agcaccggat ctaacgtgtt ccagacccgt gccggctgcc tgatcggagc
     3301 cgagcacgtg aacaactcct acgaatgcga catcccaatc ggagctggca tctgcgcttc
     3361 ttaccagacc cagaccaaca gccggagaag ggccagatcc gtggcctctc agagcatcat
     3421 cgcctacacc atgagcctgg gcgctgaaaa cagcgtggct tacagcaaca actctatcgc
     3481 catcccaacc aacttcacca tctctgtgac caccgaaatc ctgccagtga gcatgaccaa
     3541 gaccagcgtg gactgcacca tgtacatctg cggagactct accgaatgct ctaacctgct
     3601 gctgcagtac ggcagcttct gcacccagct gaaccgcgct ctgaccggaa tcgctgtgga
     3661 gcaggacaag aacacccagg aagtgttcgc tcaggtgaag cagatataca agaccccacc
     3721 tatcaaggac ttcggaggat tcaacttctc tcagatcctg cccgacccat ctaagccctc
     3781 taagaggtct ttcatcgaag acctgctgtt caacaaggtg accctggccg acgccggttt
     3841 catcaagcag tacggtgact gcctgggaga catctccgga atcaacgcct ctgtggtgaa
     3901 catccagaag gaaatcgaca gactgaacga ggtggccaag aacctgaacg aatctctgat
     3961 cgacctgcag gaactgggaa agtacgagca gtacatcaag tggccctggt acatctggct
     4021 gggtttcatc gctggtctga tcgctatcgt gatggtgacc atcatgctgt gctgcatgac
     4081 ctcttgctgc tcttgcctga agggatgctg ctcttgcgga tcttgctgca agttcgacga
     4141 ggacgactcc gagcctgtgc tgaagggtgt gaagctgcac tacacctacc caactttctt
     4201 gtacaaagtg gttgattcta gagcgatcgc cagagagctg ttcaacctgc tgggcgagaa
     4261 ccagcccccc gtggtgatca aggcgatcgc ctggagccac cctcagttcg agaagggagg
     4321 aggaagcggc ggaggcagcg gaggaggaag ctggagccac ccgcagttcg agaaaggcgg
     4381 aagcggccat caccatcacc atcaccctgc aggagaaaat ctgtacttcc aggggagagg
     4441 aggcagcgag aacctgtact ttcaggggcc tgcaggaaga gactacaaag accatgacgg
     4501 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagtagt tcgaattctc
     4561 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     4621 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     4681 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     4741 actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg
     4801 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     4861 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     4921 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     4981 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     5041 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     5101 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     5161 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     5221 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac
     5281 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5341 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5401 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5461 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5521 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     5581 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     5641 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     5701 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     5761 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     5821 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     5881 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     5941 atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6001 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     6061 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6121 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6181 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6241 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6301 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     6361 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6421 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     6481 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6541 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6601 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     6661 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6721 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6781 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     6841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6901 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6961 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7021 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7081 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7141 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7201 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     7261 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7321 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7381 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7441 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7501 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7561 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7621 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7681 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7741 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7801 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7861 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7921 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7981 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8041 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8101 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8161 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8221 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8281 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8341 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8401 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8461 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8521 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8581 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8641 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8701 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8761 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8821 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8881 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8941 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9001 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9061 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9121 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9181 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9241 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     9301 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9361 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9421 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     9481 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     9541 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     9601 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     9661 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     9721 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9781 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9841 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9901 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     9961 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
    10021 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
    10081 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
    10141 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
    10201 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
    10261 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
    10321 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
    10381 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
    10441 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
    10501 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
    10561 agcagtggga at
//
