LOCUS       sequence-422210-        8624 bp    DNA     circular SYN 14-APR-2025
DEFINITION  FRET-based reporter for Ras activity, untargeted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Zhang JZ, Nguyen WH, Greenwood N, Rose JC, Ong SE, Maly DJ, Baker D
  TITLE     Computationally designed sensors detect endogenous Ras activity and 
            signaling effectors at subcellular resolution.
  JOURNAL   Nat Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w.
  PUBMED    38273065
REFERENCE   2  (bases 1 to 8624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w."
COMMENT     Sequence Label: pcDNA3 Ras-LOCKR-S
FEATURES             Location/Qualifiers
     source          1..8624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      897..906
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             915..932
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             939..968
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             978..1007
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1017..1046
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1953..2009
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2010..2033
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2043..2066
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2076..2099
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4176..4194)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4177..4194)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4214..4231)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4220..4444
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4490..4918
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4577..4596)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4787..4808
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4927..4947)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4932..5262
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5113..5248
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5175..5194
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5329..6123
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5383..5402)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5993..6012
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6297..6418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6334..6353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6388..6407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6467..6483)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6467..6483)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6480..6502)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6491..6507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6515..6521,6522..6539,6540..6545))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6560..6581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6698..6715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6869..7457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6949..6968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7628..8419,8420..8488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8251..8270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8489..8593)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcgcca
      901 ccatggggtc tagccatcac catcatcacc attctagcga gcagaaactc atctctgaag
      961 aggatctcgg tggtagtgaa caaaagttga tatccgagga ggatttgggt gggagcgagc
     1021 agaaacttat cagtgaagaa gatttggggg gctccatgga tcctgacgag gcaaggaaag
     1081 ccatagcgag ggttaaaagg gaaagcaaac gaatagtaga agacgcggaa aggcttagtc
     1141 gagaagcagc ggcggcaagt gagaaatcct cccgagaggc ggagaggctc tcccgggagg
     1201 ccgctgctgc gtccgagaaa atttcccggg agggaggaac aggggggtca ggagggactg
     1261 gaggctcaat ggtatctaaa ggagaagagc tgtttactgg ggtcgtgcct atcctggtag
     1321 agctggacgg tgacgttaat ggacataagt ttagcgtaag tggggaaggc gaaggcgatg
     1381 ctacttacgg gaagttgact ttgaaattta tctgtaccac tggaaagctg ccggtaccct
     1441 ggcctacgtt ggttaccacg ttgacctggg gtgtccaatg cttctcaaga tacccagatc
     1501 acatgaagca acacgacttt ttcaaatctg cgatgcccga aggatatgtt caagagagaa
     1561 cgatattttt taaagatgat ggtaactata aaactagagc cgaggtcaag ttcgaggggg
     1621 acactctggt taaccggatc gaacttaaag gcattgactt taaggaggat gggaacatcc
     1681 tgggccataa attggagtac aactatatat cacataacgt ctatatcacg gcggacaagc
     1741 aaaagaatgg gataaaggct aactttaaaa tacggcataa cattgaggat ggaagcgtgc
     1801 agcttgcgga ccattatcaa caaaatacgc ctataggaga cggacccgtg ctgctgcctg
     1861 ataatcacta tctttccact caaagtgcgc tctctaaaga cccgaatgag aaacgagacc
     1921 atatggttct gttggagttc gtaacggcag cggcaaccaa tttcagtctc ctgaagcagg
     1981 caggtgatgt agaagagaat cccggaccgg attataaaga cgatgatgac aagggaggta
     2041 gtgattacaa ggatgatgac gataaaggag gttccgatta caaagatgat gacgacaagg
     2101 gcgggtctat gaagggcgaa gaacttttca caggcgtggt gccgatattg gtggaactcg
     2161 atggagacgt aaatgggcat aaattctcag tttccgggga aggggaaggt gacgccactt
     2221 atgggaagct tactctcaaa ttgctgtgca ctacggggaa gcttccagtg ccttggccta
     2281 cacttgttac cacactgggg tacggagtgc agtgtttcgc ccgctacccc gaccacatga
     2341 aacagcatga tttctttaaa tctgcgatgc cagaaggtta tgtgcaagag agaactattt
     2401 ttttcaagga tgacggcaat tacaaaacta gggcggaggt taagttcgag ggtgacacac
     2461 tcgtgaatag gattgagttg aagggcatag actttaaaga agatgggaat atcttgggtc
     2521 acaagcttga gtataattat aactcccata atgtctacat cacagcagat aaacagaaaa
     2581 atggaattaa agcaaacttc aagatacgac acaacatcga ggacggtgga gttcagttgg
     2641 cggaccacta ccaacaaaat acacctatcg gtgacgggcc agtccttctg cccgacaacc
     2701 actacctctc ctatcaaagc gcgcttttca aggaccctaa tgagaagaga gaccatatgg
     2761 ttcttcttga atttctgaca gcagcgggag gtaccggcgg ttccgggggt acgggaggca
     2821 gttcaaagga agcagcgaag aaactccagg acctgaatat agagcttgcg cggaaactcc
     2881 tcgaagcgag cacgaagctt caaagactta acataaggct ggcagaagct ctcttggaag
     2941 ccatcgctag attgcaggag ctgaatctgg agctcgtata cctggctgtt gagctgacag
     3001 accctaaacg gatacgggat gagatcaagg aagtcaaaga taagagcaag gaaattattc
     3061 gccgcgctga aaaagaaata gatgatgctg ccaaggagtc caaaaaaatt cttgaggaag
     3121 cacgaaaagc tattcgagac gccgccgagg agagtaggaa aattctcgag gaaggtagcg
     3181 ggagcgggtc agatgccctg gatgagttgc aaaagttgaa tcttgaactt gcgaagcttc
     3241 tccttaaagc aattgctgaa actcaagact tgaatcttag agctgcaaag gcctttctcg
     3301 aagcggccgc aaaattgcag gaacttaata ttagggctgt ggaacttctc gtaaagctca
     3361 ccgacccggc aaccatacgc cgggctctgg agcatgctaa gagacggtca aaggagataa
     3421 tcgacgaagc ggaaagggct attcgggcag ctaagcgcga gagcgagcga attattgaag
     3481 aagcgcggcg actcatagag aaggccaagg aggagagtga aaggataatt cgagaaggtt
     3541 ccggcagtgg tgatcccgat atcaaaaaat tgcaggatct caacattgaa ctggcaagag
     3601 agctgctccg ggctcatgca cagcttcagc gacttaattt ggagttgctt cgcgaactgc
     3661 ttcgagcttt ggctcaattg caagagctta acctcgacct cttgaggttg gcttccgaat
     3721 tgactgaccc agacgaggcg aggaaagcga tcgccgttac aggctaccgg ctctttgaag
     3781 aaatactgga cagtgagcga ttgagtcggg aggccgcagc agcatctgaa aagagcagcc
     3841 gggaggctga acgccttata cgagaagctg cagcggcttc tagcaatact ataagggttt
     3901 ttctccccaa caagcaacgc accgtggtca acgtgaggaa cgggatgtca ctgcacgatt
     3961 gcctgatgaa ggcgctgaag gttcgaggtt tgcaacccga gtgttgcgcg gtgttcaggc
     4021 ttcttcacga gcataagggg aaaaaagcac gattggattg gaacactgat gcagccagtc
     4081 tcatcggcga ggaattgcag gtggattttt tgtgagaatt ctgcagatat ccatcacact
     4141 ggcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga
     4201 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4261 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4321 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4381 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4441 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     4501 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4561 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4621 tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg
     4681 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4741 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4801 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4861 gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     4921 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga
     4981 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5041 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5101 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5161 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5221 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5281 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     5341 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5401 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5461 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     5521 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5581 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5641 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5701 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5761 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     5821 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     5881 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     5941 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6001 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6061 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6121 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6181 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6241 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6421 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6481 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6541 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6601 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6661 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6721 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6781 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6841 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6901 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6961 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7021 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     7081 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7141 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7201 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7261 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7321 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7381 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7441 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7501 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7561 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7621 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7681 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7741 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7801 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7861 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7921 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7981 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8041 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8101 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8161 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8221 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8281 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8341 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8401 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8461 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8521 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8581 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
