LOCUS       sequence-422498-        5460 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses RFP in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Becker A, Allmann L, Hofstatter M, Casa V, Weber P, Lehmkuhl A, 
            Herce HD, Cardoso MC
  TITLE     Direct homo- and hetero-interactions of MeCP2 and MBD2.
  JOURNAL   PLoS One. 2013;8(1):e53730. doi: 10.1371/journal.pone.0053730. Epub 
            2013 Jan 15.
  PUBMED    23335972
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e53730. doi"; pages: " 
            10.1371/journal.pone.0053730. Epub 2013 Jan 15"
COMMENT     Sequence Label: pFB-mRFP (pc2386)
FEATURES             Location/Qualifiers
     source          1..5460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(93..112)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     302..323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        486..590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(591..659,660..1451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(809..828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1622..2210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2111..2130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2364..2381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2515..2739
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2806..3339)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3509..3514,3515..3531,3532..3537))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3889..3980
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3905..3922
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4086..4760
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             4086..4760
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(4216..4234)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4484..4503
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4678..4701
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    4954..5088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4991..5010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5045..5064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5117..5282
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
       61 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      121 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      181 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      241 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttcttaat
      301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
      361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
      421 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
      481 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      541 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      601 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      661 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      721 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      781 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      841 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      901 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      961 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1021 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1081 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1141 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1201 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1261 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1321 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1381 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1441 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1501 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1741 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1981 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2101 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2161 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2281 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2341 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     2401 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagac cagccgcgta
     2461 acctggcaaa atcggttacg gttgagtaat aaatggatgc cctgcgtaag cgggtgtggg
     2521 cggacaataa agtcttaaac tgaacaaaat agatctaaac tatgacaata aagtcttaaa
     2581 ctagacagaa tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga
     2641 cttttgttat ggctaaagca aactcttcat tttctgaagt gcaaattgcc cgtcgtatta
     2701 aagaggggcg tggccaaggg catggtaaag actatattcg cggcgttgtg acaatttacc
     2761 gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact
     2821 tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac
     2881 tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc
     2941 ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga
     3001 gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta
     3061 agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt
     3121 actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac
     3181 gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg
     3241 gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg
     3301 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
     3361 acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc
     3421 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     3481 gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaactg
     3541 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     3601 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg
     3661 tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg
     3721 gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga
     3781 tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg cccaggactc
     3841 tagctatagt tctagtggtt ggctacgtat actccggaat attaatagat catggagata
     3901 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
     3961 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat
     4021 cccggtccga agcgcgcgga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
     4081 tcgacatggc ctcctccgag gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg
     4141 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     4201 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     4261 acatcctgtc ccctcagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca
     4321 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     4381 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     4441 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     4501 agaccatggg ctgggaggcc tccaccgagc ggatgtaccc cgaggacggc gccctgaagg
     4561 gcgagatcaa gatgaggctg aagctgaagg acggcggcca ctacgacgcc gaggtcaaga
     4621 ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc
     4681 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg
     4741 gccgccactc caccggcgcc ggcctgtaca agtaaagcgg ccgctttcga atctagagcc
     4801 tgcagtctcg aggcatgcgg taccaagctt gtcgagaagt actagaggat cataatcagc
     4861 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     4921 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4981 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5041 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
     5101 gagcctagga gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt
     5161 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     5221 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     5281 cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga
     5341 caaaccgtca tcttcggcta ctttttctct gtcacagaat gaaaattttt ctgtcatctc
     5401 ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttgcagcc tgaatggcga
//
