LOCUS       sequence-422502-        6319 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses fl. MBD3 GFP tagged in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Becker A, Allmann L, Hofstatter M, Casa V, Weber P, Lehmkuhl A, 
            Herce HD, Cardoso MC
  TITLE     Direct homo- and hetero-interactions of MeCP2 and MBD2.
  JOURNAL   PLoS One. 2013;8(1):e53730. doi: 10.1371/journal.pone.0053730. Epub 
            2013 Jan 15.
  PUBMED    23335972
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e53730. doi"; pages: " 
            10.1371/journal.pone.0053730. Epub 2013 Jan 15"
COMMENT     Sequence Label: pFBMBD3-C-GFP (pc1566)
FEATURES             Location/Qualifiers
     source          1..6319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      22..476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(109..128)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     318..339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        502..606
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(607..675,676..1467)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(825..844)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1638..2226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2127..2146
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2380..2397
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2531..2755
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2822..3355)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3525..3530,3531..3547,3548..3553))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3905..3996
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3921..3938
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(4947..4949,4950..4952,4953..5666)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4947..4949,4950..4952,4953..5663)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4992..5013)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5253..5272)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5600..5621
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5829..5963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5866..5885)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5920..5939
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5992..6157
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
       61 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      121 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      181 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      241 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
      301 agtccacgtt cttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
      361 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
      421 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
      481 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      541 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      601 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      661 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      721 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      781 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      841 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      901 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      961 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1021 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1081 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1141 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1201 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1261 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1321 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1381 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1441 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1501 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1561 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1621 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1681 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1741 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1801 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1861 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1921 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1981 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2041 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2101 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2161 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2221 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2281 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2341 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2401 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     2461 atagaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat ggatgccctg
     2521 cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg
     2581 acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt
     2641 gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa
     2701 attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc
     2761 gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt
     2821 gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact
     2881 ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag
     2941 caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc
     3001 tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa
     3061 acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa
     3121 gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt
     3181 gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg
     3241 atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta
     3301 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac
     3361 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
     3421 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
     3481 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
     3541 aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
     3601 ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt
     3661 cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt
     3721 gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg
     3781 tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt
     3841 tgttcgccca ggactctagc tatagttcta gtggttggct acgtatactc cggaatatta
     3901 atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
     3961 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
     4021 accatcgggc gcggatcccg gtccgaagcg cgcggaattc aaaggcctac gtcgacgcca
     4081 tggagcggaa gaggtgggag tgcccggcgc tcccgcaggg ctgggaaagg gaagaagtgc
     4141 ccaggaggtc ggggctgtcg gccggccaca gggatgtctt ttactatagc cccagcggga
     4201 agaagttccg cagcaagcca caactggcac gttacctggg cggatccatg gacctcagca
     4261 ccttcgactt ccgcaccgga aagatgttga tgaacaagat gaataagagt cgccagcgtg
     4321 tgcgctatga ttcttccaac caggtcaagg gcaagcctga cctgaacacc gcgctgcctg
     4381 tacggcagac tgcatccatc ttcaagcaac cggtgaccaa gatcaccaac caccccagca
     4441 acaaggtcaa gagcgacccg cagaaggcag tggaccagcc gaggcagctt ttctgggaga
     4501 agaagctaag tggattgagt gcctttgaca ttgcagaaga actggtcagg accatggact
     4561 tgcccaaggg cctgcaggga gtgggccctg gctgtacaga tgagacgctg ctgtcagcca
     4621 ttgcgagtgc tctacacacc agcaccctgc ccattacagg ccagctctct gcagccgtgg
     4681 agaagaaccc tggtgtgtgg ctgaacactg cacagccact gtgcaaagcc ttcatggtga
     4741 cagatgacga catcaggaag caggaggagc tggtacagca ggtacggaag cgcctggagg
     4801 aggcactgat ggccgacatg ctagctcatg tggaggagct tgcccgagac ggggaggcac
     4861 cactggacaa ggcctgtgca gaggaggaag aggaggagga agaggaggag gaagagccgg
     4921 agccagagcg agtgtgcggc cgcgccatgg tgagcaaggg cgaggagctg ttcaccgggg
     4981 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     5041 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     5101 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     5161 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     5221 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     5281 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     5341 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     5401 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     5461 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5521 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     5581 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5641 tcggcatgga cgagctgtac aagtaatcta gagcctgcag tctcgaggca tgcggtacca
     5701 agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac
     5761 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5821 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5881 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5941 atgtatctta tcatgtctgg atctgatcac tgcttgagcc taggagatcc gaaccagata
     6001 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     6061 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     6121 cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta
     6181 tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc ggctactttt
     6241 tctctgtcac agaatgaaaa tttttctgtc atctcttcgt tattaatgtt tgtaattgac
     6301 tgaatatcaa cgcttattt
//
