LOCUS       sequence-422573-        2364 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Empty backbone with zeocin resistance for high expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm7.1
FEATURES             Location/Qualifiers
     source          1..2364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..446
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        447..650
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     601..621
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    825..1049
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1119..1136)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1119..1135)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1139..1186
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1205..1579
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     1580..1599
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1580..1598
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1687..1705)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      1728..2316
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2217..2236
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2335..2351
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2335..2351
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtgactagcg gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac
       61 caaagggaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      121 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      181 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      241 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      301 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      361 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      421 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      481 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      541 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      601 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccgaagag
      661 ccgagtgtct tcggttcgag accgaattcg cgagctctcg ctagccagtt taaacaattg
      721 cggccgcgta ctagtctatc gatttggatc caccctgcag gaagtctaga ccgtcgacac
      781 ggtctctgag agaagacacg tcgctcttcg acgtaagtaa ctgactgtgc cttctagttg
      841 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
      901 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
      961 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     1021 gcatgctggg gatgcggtgg gctctatggc tcggtaccaa attccagaaa agaggcctcc
     1081 cgaaaggggg gccttttttc gttttggtcc taagcttaac tggccgtcgt tttacagtgt
     1141 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     1201 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     1261 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     1321 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     1381 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     1441 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     1501 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     1561 ggccgaggag caggactgat aatacgactc actatagggt aactaattga ggcgcgccta
     1621 ccaccgtcaa aaaaaacggc gctttttagc gccgttttta tttttcaacc ttcctaggta
     1681 agtgaccctt tagtgagggt taatttagaa aagatcaaag gatcttcttg agatcctttt
     1741 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1801 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1861 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1921 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1981 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2041 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2101 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2161 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2221 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2281 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggcccaggaa
     2341 acagctatga ccatgcccgg gtaa
//
