LOCUS       sequence-422647-       11195 bp    DNA     circular SYN 15-APR-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn5 with GmR and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11195)
  TITLE     HOLD-Jaeger Lab pYT plasmids
REFERENCE   2  (bases 1 to 11195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYTRW13K_3G5
FEATURES             Location/Qualifiers
     source          1..11195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1689..2399
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1834..1852)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2102..2121
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        join(2571..2576,2577..2593,2594..2599)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2788..3321
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     misc_feature    5496..5788
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      6436..6889
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(6917..6939)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7025..7165
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7080..7099
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7180..7197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7351..7939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7431..7450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8118..8912)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8229..8248)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8839..8858
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        9350..9565
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             9566..10369
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgaac tttagaagga
     1681 gatataccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     1741 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     1801 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     1861 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     1921 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     1981 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctcct
     2041 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2101 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2161 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2221 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     2281 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     2341 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaac
     2401 gcgcgcgtaa ctataacggt cctaaggtag cgagcttact tgtgctgttg tgtaaggaca
     2461 cgcttaaccc agagaattcc agcgacctat tctacgacta cgcgtccatg gctcgaggag
     2521 ctcggtaccg ccccactacc cgatttatca agatttggcg gagagtccca ttgacataag
     2581 cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2641 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2701 gtttttttgt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2761 gatgtttgat gttatggagc agcaacgatg ttacgcagca gcaacgatgt tacgcagcag
     2821 ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg cacatgtagg
     2881 ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg tcgtgagttc
     2941 ggagacgtag ccacctactc ccaacatcag ccggactccg attacctcgg gaacttgctc
     3001 cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc
     3061 gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg agatctatat ctatgatctc
     3121 gcagtctccg gcgagcaccg gaggcagggc attgccaccg cgctcatcaa tctcctcaag
     3181 catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta cggtgacgat
     3241 cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca ctttgatatc
     3301 gacccaagta ccgccaccta agtcgactgg caaacagcta ttatgggtat tatgggtgtg
     3361 tatccagaac gacggatctt gacgcaattt ggcgtggctc tcttaaggta gccctccgac
     3421 caattgaggt gcgctctgac cgatcagttt agacttgtgt ataagagtca gagcgtcctg
     3481 aacggaacct ttcccgtttt ccaggatctg atcttccatg tgacctccta acatggtaac
     3541 gttcatgata acttctgctc ttcatcgtgc ggccgactgg gctaaatctg tgttctcttc
     3601 ggcggcgctg ggtgatcctc gccgtactgc ccgcttggtt aacgtcgccg cccaattggc
     3661 aaaatattct ggtaaatcaa taaccatctc atcagagggt agtgaagcca tgcaggaagg
     3721 cgcttaccga tttatccgca atcccaacgt ttctgccgag gcgatcagaa aggctggcgc
     3781 catgcaaaca gtcaagttgg ctcaggagtt tcccgaactg ctggccattg aggacaccac
     3841 ctctttgagt tatcgccacc aggtcgccga agagcttggc aagctgggct ctattcagga
     3901 taaatcccgc ggatggtggg ttcactccgt tctcttgctc gaggccacca cattccgcac
     3961 cgtaggatta ctgcatcagg agtggtggat gcgcccggat gaccctgccg atgcggatga
     4021 aaaggagagt ggcaaatggc tggcagcggc cgcaactagc cggttacgca tgggcagcat
     4081 gatgagcaac gtgattgcgg tctgtgaccg cgaagccgat attcatgctt atctgcagga
     4141 caaactggcg cataacgagc gcttcgtggt gcgctccaag cacccacgca aggacgtaga
     4201 gtctgggttg tatctgtacg accatctgaa gaaccaaccg gagttgggtg gctatcagat
     4261 cagcattccg caaaagggcg tggtggataa acgcggtaaa cgtaaaaatc gaccagcccg
     4321 caaggcgagc ttgagcctgc gcagtgggcg catcacgcta aaacagggga atatcacgct
     4381 caacgcggtg ctggccgagg agattaaccc gcccaagggt gagaccccgt tgaaatggtt
     4441 gttgctgacc agcgaaccgg tcgagtcgct agcccaagcc ttgcgcgtca tcgacattta
     4501 tacccatcgc tggcggatcg aggagttcca taaggcatgg aaaaccggag caggagccga
     4561 gaggcaacgc atggaggagc cggataatct ggagcggatg gtctcgatcc tctcgtttgt
     4621 tgcggtcagg ctgttacagc tcagagaaag cttcacgctg ccgcaagcac tcagggcgca
     4681 agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc
     4741 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc
     4801 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
     4861 gcgaaccgga attgccagct ggggcgccct ctgggaaggt tgggaagccc tgcaaagtaa
     4921 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgacccgg
     4981 gactagttgg tgtggaagtc ctaatatcgg tgatcaatat agtggttgac atgctggcta
     5041 gtcaacattg agccttttga tcatgcaaat atattacggt attttacaat caaatatcaa
     5101 acttaactat tgactttata acttatttag gtggtaacat tcttataaaa aagaaaaaaa
     5161 ttactgcaaa acagtactag cttttaactt gtatcctagg ttatctatgc tgtctcacca
     5221 tagagaatat tacctatttc agaatgtatg tccatgattc gccgggtaaa tacatataat
     5281 acacaaatct ggcttaataa agtctataat atatctcata aagaagtgct aaattggcta
     5341 gtgctatata tttttaagaa aatttctttt gactaagtcc atatcgactt tgtaaaagtt
     5401 cacattagca tacatatatt acacgagcca gaaatagtaa cttttgccta aatcacaaat
     5461 tgcaaaattt aattgcttgc aaaaggtcac atgcttataa tcaacttttt taaaaattta
     5521 aaatactttt ttatttttta tttttaaaca taaatgaaat aatttattta ttgtttatga
     5581 ttaccgaaac ataaaacctg ctcaagaaaa agaaactgtt ttgtccttgg aaaaaaagca
     5641 ctacctagga gcggccaaaa tgccgaggct ttcatagctt aaactcttta cagaaaatag
     5701 gcattataga tcagttcgag ttttcttatt cttccttccg gttttatcgt cacagtttta
     5761 cagtaaataa gtatcacctc ttagagttaa cctatactaa aatttatgcc agttaactat
     5821 gagataagca agtatcatct catttcatta cctgaagtcg agtaaacaga aaatccaatt
     5881 gttgatgaac ctcaatgact tagaactatc tatcggcaga tcatataaag aggatttagg
     5941 tactcgagaa cgctccgtac atgcagctaa acctccaagc atctctaact tgggtagtgc
     6001 tttctccacc attttctgtg cttgctcctt cgtggcaagt ccagcccata atgcccagaa
     6061 tgtagttgcg gattcgtatg acgttctgtg cttgattttt gtgttgtagt caaagaaaag
     6121 gtccggttaa acggatctcg cattgatgag gcaacgctaa ttatcaacat atagattgtt
     6181 atctatctgc atgaacacga aatctttact tgacgacttg aggctgatgg tgtttatgca
     6241 aagaaaccac tgtgtttaat atgtgtcact gtttgatatt actgtcagcg tagaagataa
     6301 tagtaaaagc ggttaataag tgtatttgag ataagtgtga taaagttttt acagcgaaaa
     6361 gacgataaat acaagaaaat gattacgagg atacggagag aggtatgtac atgtgtattt
     6421 atatactaag ctgccggcgg ttgtttgcaa gaccgagaaa aggctagcaa gaatcgggtc
     6481 attgtagcgt atgcgcctgt gaacattctc ttcaacaagt ttgattccat tgcggtgaaa
     6541 tggtaaaagt caaccccctg cgatgtatat tttcctgtac aatcaatcaa aaagccaaat
     6601 gatttagcat tatctttaca tcttgttatt ttacagattt tatgtttaga tcttttatgc
     6661 ttgcttttca aaaggcctgc aggcaagtgc acaaacaata cttaaataaa tactactcag
     6721 taataaccta tttcttagca tttttgacga aatttgctat tttgttagag tcttttacac
     6781 catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat
     6841 caccaacatt ttctggcgtc agtccaccag ctaacataaa atgttgcctc gcgcgtttcg
     6901 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     6961 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     7021 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     7081 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     7141 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7201 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7261 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     7801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8041 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8101 gtctgacagg agctcgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     8161 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     8221 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     8281 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     8341 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt gagcctggcg
     8401 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     8461 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     8521 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     8581 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     8641 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     8701 gccagccacg atagccgcgc tgcctcgtct tggagttcat tcagggcacc ggacaggtcg
     8761 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     8821 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     8881 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     8941 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     9001 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     9061 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     9121 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccgggcg
     9181 atcgatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat
     9241 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg
     9301 tccgccatct ccagcagccg cacgcggcgc atcggggggg ggggggggtt tcaattcatc
     9361 attttttttt tattcttttt tttgatttcg gtttccttga aatttttttg attcggtaat
     9421 ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat
     9481 atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa
     9541 aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac
     9601 tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt
     9661 gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg
     9721 tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg
     9781 cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag
     9841 aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat
     9901 agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg
     9961 tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga
    10021 attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc
    10081 gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga
    10141 tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc
    10201 attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat
    10261 tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag
    10321 aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt
    10381 ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt
    10441 attacccggg aatctcggtc gtaatgattt ttataatgac gaaaaaaaaa aaattggaaa
    10501 gaaaaccccc ccccccgcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg
    10561 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg
    10621 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca
    10681 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc
    10741 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
    10801 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
    10861 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
    10921 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
    10981 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
    11041 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
    11101 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg ctgggccatt
    11161 ctcatgaaga atatcttgaa tttattgtca tatta
//
