LOCUS       sequence-422743-        5932 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses MBD2 (aa1-152) GFP tagged in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Becker A, Allmann L, Hofstatter M, Casa V, Weber P, Lehmkuhl A, 
            Herce HD, Cardoso MC
  TITLE     Direct homo- and hetero-interactions of MeCP2 and MBD2.
  JOURNAL   PLoS One. 2013;8(1):e53730. doi: 10.1371/journal.pone.0053730. Epub 
            2013 Jan 15.
  PUBMED    23335972
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e53730. doi"; pages: " 
            10.1371/journal.pone.0053730. Epub 2013 Jan 15"
COMMENT     Sequence Label: pFB_MBD2.1-GFP (pc2082)
FEATURES             Location/Qualifiers
     source          1..5932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      8..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(95..114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        488..592
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(593..661,662..1453)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(811..830)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1624..2212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2113..2132
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2366..2383
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2517..2741
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2808..3341)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3511..3516,3517..3533,3534..3539))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3891..3982
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3907..3924
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(4546..4548,4549..4551,4552..5265)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4546..4548,4549..4551,4552..5262)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4591..4612)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4852..4871)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5199..5220
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5428..5562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5465..5484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5519..5538
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5591..5756
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
       61 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      121 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      181 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
      241 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttctta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
      481 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      541 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      601 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      661 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      721 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      781 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      841 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      901 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      961 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1021 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1081 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     1141 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1201 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     1261 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     1321 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1381 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     1441 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1501 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1561 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1621 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1681 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1741 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1801 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1861 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1981 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2041 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2161 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2221 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2281 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2341 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     2401 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag accagccgcg
     2461 taacctggca aaatcggtta cggttgagta ataaatggat gccctgcgta agcgggtgtg
     2521 ggcggacaat aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta
     2581 aactagacag aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg
     2641 gacttttgtt atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat
     2701 taaagagggg cgtggccaag ggcatggtaa agactatatt cgcggcgttg tgacaattta
     2761 ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta
     2821 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc
     2881 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg
     2941 gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg
     3001 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg
     3061 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc
     3121 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct
     3181 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca
     3241 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg
     3301 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     3361 taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa
     3421 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     3481 ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaac
     3541 tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc
     3601 gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat
     3661 cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc
     3721 tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg
     3781 gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccaggac
     3841 tctagctata gttctagtgg ttggctacgt atactccgga atattaatag atcatggaga
     3901 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
     3961 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
     4021 atcccggtcc gaagcgcgcg gaattcaaag gcctacgtcg acgccatgag cgatcgcatg
     4081 cgcgcgcacc cggggggagg ccgctgctgc ccggagcagg aggaggggga gagcgcggcg
     4141 ggcggcagcg gcgctggcgg cgactccgcc atagagcagg ggggccaggg cagcgcgctc
     4201 gctccgtccc cggtgagcgg cgtgcgcagg gaaggcgctc ggggcggcgg ccgtggccgg
     4261 gggcggtgga agcaggcggc ccggggcggc ggcgtctgtg gccgtggccg tggccgtggc
     4321 cggggtcggg gccgtggccg gggccggggc cggggccgcg gccgtcccca gagtggcggc
     4381 agcggccttg gcggcgacgg cggcggcggc gcgggcggct gcggcgtcgg cagcggtggc
     4441 ggcgtcgccc cccggcggga tcctgtccct ttcccgtcgg ggagctcggg gccggggccc
     4501 aggggacccc gggccacgga gagcgggaag agggcggccg cagccatggt gagcaagggc
     4561 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4621 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4681 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     4741 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     4801 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4861 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4921 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4981 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     5041 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5101 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5161 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5221 accgccgccg ggatcactct cggcatggac gagctgtaca agtaatctag agcctgcagt
     5281 ctcgaggcat gcggtaccaa gcttgtcgag aagtactaga ggatcataat cagccatacc
     5341 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     5401 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     5461 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5521 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct
     5581 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
     5641 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
     5701 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg
     5761 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
     5821 gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt
     5881 attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaatg gc
//
