LOCUS       sequence-422747-        5696 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses MBD2 (aa153-235) RFP tagged in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Becker A, Allmann L, Hofstatter M, Casa V, Weber P, Lehmkuhl A, 
            Herce HD, Cardoso MC
  TITLE     Direct homo- and hetero-interactions of MeCP2 and MBD2.
  JOURNAL   PLoS One. 2013;8(1):e53730. doi: 10.1371/journal.pone.0053730. Epub 
            2013 Jan 15.
  PUBMED    23335972
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e53730. doi"; pages: " 
            10.1371/journal.pone.0053730. Epub 2013 Jan 15"
COMMENT     Sequence Label: pFB-MBD2.6R (pc2397)
FEATURES             Location/Qualifiers
     source          1..5696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..461
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     303..324
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        487..591
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(592..660,661..1452)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(810..829)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1623..2211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2112..2131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2365..2382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2516..2740
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2807..3340)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3510..3515,3516..3532,3533..3538))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3890..3981
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3906..3923
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4323..4997
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             4323..4997
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(4453..4471)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4721..4740
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4915..4938
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    5191..5325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5228..5247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5282..5301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5354..5519
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttaa
      301 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      361 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
      421 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
      481 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      541 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      601 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      661 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      721 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      781 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      841 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      901 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      961 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1021 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1081 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1141 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1201 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1261 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     1321 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1381 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1441 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1501 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1561 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1621 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1681 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1741 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     1801 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1861 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1921 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1981 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2041 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2101 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2161 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2221 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2281 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2341 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     2401 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga ccagccgcgt
     2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg
     2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa
     2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg
     2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt
     2701 aaagaggggc gtggccaagg gcatggtaaa gactatattc gcggcgttgt gacaatttac
     2761 cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
     2821 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
     2881 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
     2941 cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
     3001 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
     3061 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
     3121 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
     3181 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
     3241 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
     3301 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     3361 aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag tcataacaag
     3421 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
     3481 tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact
     3541 gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg
     3601 aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc tccacgcatc
     3661 gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg gatctgccct
     3721 ggcttcagga gatcggaaga cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg
     3781 atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc gcccaggact
     3841 ctagctatag ttctagtggt tggctacgta tactccggaa tattaataga tcatggagat
     3901 aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt
     3961 gtaataaaaa aacctataaa tattccggat tattcatacc gtcccaccat cgggcgcgga
     4021 tcccggtccg aagcgcgcgg aattcaaagg cctacgtcga catggactgc ccggccctcc
     4081 cccccggatg gaagaaggag gaagtgatcc gaaaatcagg gctcagtgct ggcaagagcg
     4141 atgtctacta cttcagtcca agtggtaaga agttcagaag taaacctcag ctggcaagat
     4201 acctgggaaa tgctgttgac cttagcagtt ttgacttcag gaccggcaag atgatgccta
     4261 gtaaattaca gaagaacaag cagagactcc ggaatgaccc cctcaatcag aaaccggtcg
     4321 acatggcctc ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg
     4381 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     4441 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     4501 tcctgtcccc tcagttccag tacggctcca aggcctacgt gaagcacccc gccgacatcc
     4561 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     4621 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     4681 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     4741 ccatgggctg ggaggcctcc accgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     4801 agatcaagat gaggctgaag ctgaaggacg gcggccacta cgacgccgag gtcaagacca
     4861 cctacatggc caagaagccc gtgcagctgc ccggcgccta caagaccgac atcaagctgg
     4921 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc gccgagggcc
     4981 gccactccac cggcgccggc ctgtacaagt aaagcggccg ctttcgaatc tagagcctgc
     5041 agtctcgagg catgcggtac caagcttgtc gagaagtact agaggatcat aatcagccat
     5101 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     5161 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     5221 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5281 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag
     5341 cctaggagat ccgaaccaga taagtgaaat ctagttccaa actattttgt catttttaat
     5401 tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa
     5461 taatccttaa aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag
     5521 cggggcattt ttcttcctgt tatgttttta atcaaacatc ctgccaactc catgtgacaa
     5581 accgtcatct tcggctactt tttctctgtc acagaatgaa aatttttctg tcatctcttc
     5641 gttattaatg tttgtaattg actgaatatc aacgcttatt tgcagcctga atggcg
//
