LOCUS       sequence-42284-e         849 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SMYD3
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaatattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tggagccgct gaaggtggaa aagttcgcaa
      121 ccgccaacag gggaaacggg ctgcgcgccg tgaccccgct gcgccccgga gagctactct
      181 tccgctcgga tcccttggcg tacacggtgt gcaaggggag tcgtggcgtc gtctgcgacc
      241 gctgccttct cgggaaggaa aagctgatgc gatgctctca gtgccgcgtc gccaaatact
      301 gtagtgctaa gtgtcagaaa aaagcttggc cagaccacaa gcgggaatgc aaatgcctta
      361 aaagctgcaa acccagatat cctccagact ccgttcgact tcttggcaga gttgtcttca
      421 aacttatgga tggagcacct tcagaatcag agaagcttta ctcattttat gatctggagt
      481 caaatattaa caaactgact gaagatagga aagagggcct caggcaactc gtaatgacat
      541 ttcaacattt catgagagaa gaaatacagg atgcctctca gctgccacct gcctttgacc
      601 tttttgaagc ctttgcaaaa gtgatctgca actctttcac catctgtaat gcggagatgc
      661 aggaagttgg tgttggccta tatcccagta tctctttgct caatcacagc tgtgacccca
      721 actgttcgat tgtgttcaat gggccccacc tcttactgcg agcagtccga gacatcgagg
      781 tgggagagga gctcaccatc tgctacctgg atatgctgat gaccagtgag gagcgccgga
      841 agcagctga
//
