LOCUS       sequence-423597-        9951 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses V5-tagged mutant version of EZH1 with deletion of aa 
            744-747 and partially resistant to JQ-EZ-05 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Chakraborty AA, Nakamura E, Qi J, Creech A, Jaffe JD, Paulk J, Novak
            JS, Nagulapalli K, McBrayer SK, Cowley GS, Pineda J, Song J, Wang 
            YE, Carr SA, Root DE, Signoretti S, Bradner JE, Kaelin WG Jr
  TITLE     HIF activation causes synthetic lethality between the VHL tumor 
            suppressor and the EZH1 histone methyltransferase.
  JOURNAL   Sci Transl Med. 2017 Jul 12;9(398):eaal5272. doi: 
            10.1126/scitranslmed.aal5272.
  PUBMED    28701475
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2017-07-12"; volume: "9(398):eaal5272. doi"; pages: " 
            10.1126/scitranslmed.aal5272"
COMMENT     Sequence Label: pLX304-EZH1-dPSET
FEATURES             Location/Qualifiers
     source          1..9951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2850..2874)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2876..2917
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2959..3547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3012..3032)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3430..3441)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3619..3852
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3930..4051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3967..3986)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4021..4040
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4091..4226
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4246..4265)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4247..4265)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4275..4292)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4275..4291)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4284..4306)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4433..4888
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4520..4539)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4730..4751
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4914..5018
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5019..5087,5088..5879)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5237..5256)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6050..6638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6539..6558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6792..6809
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6926..6947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6962..6967,6968..6985,6986..6992)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7000..7016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7005..7027
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7024..7040
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7024..7040
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7059..7079
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7061..7079
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7107..7333
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7334..7514
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7561..7686
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8179..8412
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8597..8641
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8816..9316
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9235..9253
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9328..9723
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9783..9900
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag
      601 tttgtacaaa aaagttggca tggaaatacc aaatccccct acctccaaat gtatcactta
      661 ctggaaaaga aaagtgaaat ctgaatacat gcgacttcga caacttaaac ggcttcaggc
      721 aaatatgggt gcaaaggctt tgtatgtggc aaattttgca aaggttcaag aaaaaaccca
      781 gatcctcaat gaagaatgga agaagcttcg tgtccaacct gttcagtcaa tgaagcctgt
      841 gagtggacac ccttttctca aaaagtgtac catagagagc attttcccgg gatttgcaag
      901 ccaacatatg ttaatgaggt cactgaacac agttgcattg gttcccatca tgtattcctg
      961 gtcccctctc caacagaact ttatggtaga agatgagacg gttttgtgca atattcccta
     1021 catgggagat gaagtgaaag aagaagatga gacttttatt gaggagctga tcaataacta
     1081 tgatgggaaa gtccatggtg aagaagagat gatccctgga tccgttctga ttagtgatgc
     1141 tgtttttctg gagttggtcg atgccctgaa tcagtactca gatgaggagg aggaagggca
     1201 caatgacacc tcagatggaa agcaggatga cagcaaagaa gatctgccag taacaagaaa
     1261 gagaaagcga catgctattg aaggcaacaa aaagagttcc aagaaacagt tcccaaatga
     1321 catgatcttc agtgcaattg cctcaatgtt ccctgagaat ggtgtcccag atgacatgaa
     1381 ggagaggtat cgagaactaa cagagatgtc agaccccaat gcacttcccc ctcagtgcac
     1441 acccaacatc gatggcccca atgccaagtc tgtgcagcgg gagcaatctc tgcactcctt
     1501 ccacacactt ttttgccggc gctgctttaa atacgactgc ttccttcacc cttttcatgc
     1561 cacccctaat gtatataaac gcaagaataa agaaatcaag attgaaccag aaccatgtgg
     1621 cacagactgc ttccttttgc tggaaggagc aaaggagtat gccatgctcc acaacccccg
     1681 ctccaagtgc tctggtcgtc gccggagaag gcaccacata gtcagtgctt cctgctccaa
     1741 tgcctcagcc tctgctgtgg ctgagactaa agaaggagac agtgacaggg acacaggcaa
     1801 tgactgggcc tccagttctt cagaggctaa ctctcgctgt cagactccca caaaacagaa
     1861 ggctagtcca gccccacctc aactctgcgt agtggaagca ccctcggagc ctgtggaatg
     1921 gactggggct gaagaatctc tttttcgagt cttccatggc acctacttca acaacttctg
     1981 ttcaatagcc aggcttctgg ggaccaagac gtgcaagcag gtctttcagt ttgcagtcaa
     2041 agaatcactt atcctgaagc tgccaacaga tgagctcatg aacccctcac agaagaagaa
     2101 aagaaagcac agattgtggg ctgcacactg caggaagatt cagctgaaga aagataactc
     2161 ttccacacaa gtgtacaact accaaccctg cgaccaccca gaccgcccct gtgacagcac
     2221 ctgcccctgc atcatgactc agaatttctg tgagaagttc tgccagtgca acccagactg
     2281 tcagaatcgt ttccctggct gtcgctgtaa gacccagtgc aataccaagc aatgtccttg
     2341 ctatctggca gtgcgagaat gtgaccctga cctgtgtctc acctgtgggg cctcagagca
     2401 ctgggactgc aaggtggttt cctgtaaaaa ctgcagcatc cagcgtggac ttaagaagca
     2461 cctgctgctg gccccctctg atgtggccgg atggggcacc ttcataaagg agtctgtgca
     2521 gaagaacgaa ttcatttctg aatactgtgg tgagctcatc tctcaggatg aggctgatcg
     2581 acgcggaaag gtctatgaca aatacatgtc cagcttcctc ttcaacctca ataatgattt
     2641 tgtagtggat gctactcgga aaggaaacaa aattcgattt gcaaatcatt cagtgaatcc
     2701 caactgttat gccaaagtgg tcatggtgaa tggagaccat cggattggga tctttgccaa
     2761 gagggcaatt caagctggcg aagagctctt ctttgattac aggtacagcc aagctgatgc
     2821 tctcaagtac gtggggatcg agagggagtg cccaactttc ttgtacaaag tggttggtaa
     2881 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg
     2941 gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3001 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3061 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3121 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3181 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3241 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3301 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3361 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3421 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3481 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3541 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3601 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3661 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3721 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3781 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3841 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     3901 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     3961 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4021 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4081 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4141 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4201 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4261 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4321 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4381 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4441 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4501 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4561 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4621 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4681 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4741 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4801 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4861 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4921 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4981 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5041 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5101 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5161 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5221 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5281 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5341 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5401 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5461 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5521 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5581 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5641 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5701 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5761 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5821 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5881 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5941 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6001 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6061 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6181 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6661 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6721 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6781 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6841 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6901 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6961 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7021 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7081 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     7141 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7201 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     7261 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     7321 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7381 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7441 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7501 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7561 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7621 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7681 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7741 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7801 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7861 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7921 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7981 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     8041 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8101 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8161 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8221 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8281 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8341 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8401 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8461 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8521 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8581 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8641 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8701 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8761 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccttgcg
     8821 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     8881 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     8941 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     9001 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     9061 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     9121 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     9181 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     9241 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     9301 cgacctctct ccccaggggg taccaccatg gccaagcctt tgtctcaaga agaatccacc
     9361 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     9421 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     9481 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     9541 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     9601 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     9661 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     9721 ggcctgcagc tgcagtagta agaattctag atcttgagac aaatggcagt attcatccac
     9781 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     9841 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     9901 cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg g
//
