LOCUS       sequence-424118-        9380 bp    DNA     circular SYN 15-APR-2025
DEFINITION  RADARSv3 driving mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Jiang K, Koob J, Chen XD, Krajeski RN, Zhang Y, Volf V, Zhou W, 
            Sgrizzi SR, Villiger L, Gootenberg JS, Chen F, Abudayyeh OO
  TITLE     Programmable eukaryotic protein synthesis with RNA sensors by 
            harnessing ADAR.
  JOURNAL   Nat Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 
            10.1038/s41587-022-01534-5.
  PUBMED    36302988
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 
            10.1038/s41587-022-01534-5."
COMMENT     Sequence Label: pKJ276
FEATURES             Location/Qualifiers
     source          1..9380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    109..130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(145..150,151..168,169..175)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    183..199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     188..210
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     207..223
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     207..223
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    237..285
                     /note="synthetic polyadenylation signal"
     CDS             complement(286..1947)
                     /codon_start=1
                     /product="secreted Cypridina luciferase"
                     /label=CLuc
                     /note="contains 30 codon substitutions for efficient 
                     translation in mammalian cells"
                     /translation="MKTLILAVALVYCATVHCQDCPYEPDPPNTVPTSCEAKEGECIDS
                     SCGTCTRDILSDGLCENKPGKTCCRMCQYVIECRVEAAGWFRTFYGKRFQFQEPGTYVL
                     GQGTKGGDWKVSITLENLDGTKGAVLTKTRLEVAGDIIDIAQATENPITVNGGADPIIA
                     NPYTIGEVTIAVVEMPGFNITVIEFFKLIVIDILGGRSVRIAPDTANKGMISGLCGDLK
                     MMEDTDFTSDPEQLAIQPKINQEFDGCPLYGNPDDVAYCKGLLEPYKDSCRNPINFYYY
                     TISCAFARCMGGDERASHVLLDYRETCAAPETRGTCVLSGHTFYDTFDKARYQFQGPCK
                     EILMAADCFWNTWDVKVSHRNVDSYTEVEKVRIRKQSTVVELIVDGKQILVGGEAVSVP
                     YSSQNTSIYWQDGDILTTAILPEALVVKFNFKQLLVVHIRDPFDGKTCGICGNYNQDFS
                     DDSFDAEGACDLTPNPPGCTEEQKPEAERLCNSLFAGQSDLDQKCNVCHKPDRVERCMY
                     EYCLRGQQGFCDHAWEFKKECYIKHGDTLEVPDECK"
     primer_bind     complement(1972..1991)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1973..1991)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2036..2239)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2065..2085)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2240..2619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2733..3910
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2964..3901
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3858..3878
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3923..3932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3929..4636
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4074..4092)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4342..4361
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4637..4690
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_RNA        4768..4786
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             4943..4999
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5009..5563
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="GVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRGK
                     LPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQ
                     GGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQR
                     CATFASKIQGQVDKIKGAGGD"
     misc_feature    5564..6131
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5581..6133
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5725..5742)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5952..5971
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6145..6744
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6145..6164)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6641..6661
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6753..6977
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6987..7004)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6987..7003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6996..7018)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7212..7231)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7331..7353
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7391..7409)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7477..7581
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7582..7650,7651..8442)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSGGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7800..7819)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8613..9201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9102..9121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9355..9372
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
       61 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      121 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      181 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccacaca
      241 caaaaaacca acacacagat gtaatgaaaa taaagatatt ttattttatt tgcattcatc
      301 tggtacttct agggtgtctc catgctttat gtagcattct ttcttgaact cccatgcgtg
      361 gtcacagaaa ccctgttgtc ccctcaggca atactcgtac atgcatcgtt cgacacggtc
      421 aggcttgtgg cacacgttac atttctgatc aagatcactt tgaccggcga agagactatt
      481 gcagagtcgt tcagcttcag gtttctgttc ttcggtgcat cccggtgggt tgggggtcag
      541 atcacaggct ccttcagcat caaaagaatc atcactgaaa tcctggttgt agttaccgca
      601 aataccgcaa gtcttaccat cgaatggatc tctaatatgt acgacgagca gttgcttgaa
      661 gttgaacttg accaccagag cttcaggtag gatggctgta gtcagtatgt caccatcttg
      721 ccagtagatg gaagtgttct gagagctgta cgggacggac acggcttctc ctccaaccag
      781 aatctgtttt ccatcaacaa tgagttctac tacagtcgat tgtttcctga ttcgtacttt
      841 ctctacttca gtgtaagagt caacattcct gtgtgaaacc ttcacatccc aagtgttcca
      901 gaaacagtcg gcggccataa gaatctcctt gcagggaccc tggaattggt atcttgcttt
      961 gtcaaatgta tcgtagaaag tatgtccaga caaaacgcag gttcctctag tttcgggagc
     1021 agcgcacgtc tccctgtagt caagcagcac gtgtgaggct cgctcgtctc cacccataca
     1081 gcgggcgaag gcgcaggaga tggtgtagta gtagaagttg atggggttgc ggcagctgtc
     1141 cttgtacggc tccagaagac ctttgcagta tgcaacgtca tcaggatttc catagagtgg
     1201 acaaccgtca aactcctggt tgatcttagg ctgaatagcg agttgttctg gatctgaagt
     1261 gaagtctgta tcttccatca ttttaagatc tccacagagg ccagagatca ttcctttgtt
     1321 tgctgtgtct ggggcgattc ttacagatct tcctccgagg atgtcgatca cgatcagttt
     1381 gaagaactca atgacggtga tgttgaagcc tggcatctca acaacagcga tggtgacctc
     1441 gccgatggtg tacgggttgg cgatgatagg gtcagctcca ccgtttacag tgatgggatt
     1501 ctcagtagct tgagcgatgt caatgatgtc tccagccact tccagtcttg tcttggtcag
     1561 cacagccccc ttggttccat ccaggttctc cagggtgatg gacaccttcc agtcgccgcc
     1621 cttggttcct tgacccaaca cgtatgtacc aggttcctgg aactggaatc tctttccata
     1681 gaatgttcta aaccatcctg cggcctctac tctgcattca attacatact gacacattcg
     1741 gcaacatgtt tttcctggtt tattttcaca cagtccatct gatagtatgt ctctcgtgca
     1801 ggtgccacag ctgctatcaa tacattctcc ttctttagct tcacaggaag ttggaactgt
     1861 gtttggtgga tcaggttcgt aaggacagtc ctggcaatga acagtggcgc agtagactaa
     1921 tgcaacggca agaattaagg tcttcatggt ggcggtacca agcttgcgtc tccctatagt
     1981 gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc
     2041 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     2101 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     2161 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     2221 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     2281 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     2341 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     2401 tttaccgtaa atagtccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     2461 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     2521 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     2581 taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat ctggcccgta
     2641 catcgaagct gatgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
     2701 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
     2761 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     2821 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     2881 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     2941 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     3001 ttacgggtta tggcccttgc gtgccttgaa ttacttccac tggctgcagt acgtgattct
     3061 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc
     3121 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat
     3181 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt
     3241 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga
     3301 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca
     3361 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta
     3421 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc
     3481 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
     3541 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga
     3601 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
     3661 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc
     3721 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
     3781 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
     3841 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
     3901 ggtgtcgtga cgtacgggat ccgccaccat ggtgagcaag ggcgaggagg ataacatggc
     3961 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     4021 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     4081 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     4141 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     4201 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     4261 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     4321 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     4381 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     4441 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     4501 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     4561 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     4621 ggacgagctg tacaaggagg gcaggggaag tcttctaaca tgcggggacg tggaggaaaa
     4681 tcccggcccc tctagaactc caaaagacca gtggtggttt tcgacgcagg accaccgcgt
     4741 cggcaagtct cctcattgga tccagataca tgaggatcac ccatgtgcat ccatcttttt
     4801 cagccatctt tagaaggttc aggttgtttt ctgccaggac gcaggaccac cgcgtccttt
     4861 gctgctttca cacaggttac tcacatgggg atcacccagg tacaggtctc ctttctcagg
     4921 gctgagagct agcggaagcg gagctactaa cttcagcctg ctgaagcagg ctggcgacgt
     4981 ggaggagaac cctggacctg taagtaaggg agtcaaagtt ctgtttgccc tgatctgcat
     5041 cgctgtggcc gaggccaagc ccaccgagaa caacgaagac ttcaacatcg tggccgtggc
     5101 cagcaacttc gcgaccacgg atctcgatgc tgaccgcggg aagttgcccg gcaagaagct
     5161 gccgctggag gtgctcaaag agatggaagc caatgcccgg aaagctggct gcaccagggg
     5221 ctgtctgatc tgcctgtccc acatcaagtg cacgcccaag atgaagaagt tcatcccagg
     5281 acgctgccac acctacgaag gcgacaaaga gtccgcacag ggcggcatag gcgaggcgat
     5341 cgtcgacatt cctgagattc ctgggttcaa ggacttggag cccatggagc agttcatcgc
     5401 acaggtcgat ctgtgtgtgg actgcacaac tggctgcctc aaagggcttg ccaacgtgca
     5461 gtgttctgac ctgctcaaga agtggctgcc gcaacgctgt gcgacctttg ccagcaagat
     5521 ccagggccag gtggacaaga tcaagggggc cggtggtgac taatccctcc ccccccccta
     5581 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     5641 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     5701 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     5761 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     5821 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     5881 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     5941 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     6001 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     6061 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     6121 cacgatgata atatggccac acatatgacc gagtacaagc ccacggtgcg cctcgccacc
     6181 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6241 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6301 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6361 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6421 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6481 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6541 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6601 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6661 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     6721 atgacccgca agcccggtgc ctaagcggcc gcctgtgcct tctagttgcc agccatctgt
     6781 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6841 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6901 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6961 tgcggtgggc tctatggctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa
     7021 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7081 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7141 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     7201 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     7261 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     7321 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     7381 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     7441 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7501 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7561 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7621 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7681 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7741 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7801 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     7861 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7921 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7981 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8041 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8101 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8161 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8221 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8281 ggagccggtg agcgtgggtc tggcggtatc attgcagcac tggggccaga tggtaagccc
     8341 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8401 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8461 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8521 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9001 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9241 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9361 tcagtgagcg aggaagcgga
//
