LOCUS       sequence-424338-        9020 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses FLAG epitope-tagged OeNL ""Orange"" BRET reporter in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Labra B, Parag-Sharma K, Powers JJ, Srivastava S, Walker JR, 
            Kirkland TA, Brennan CK, Prescher JA, Amelio AL
  TITLE     Optimized in vivo multispectral bioluminescent imaging of tumor 
            biology using engineered BRET reporters.
  JOURNAL   iScience. 2024 Aug 8;27(9):110655. doi: 10.1016/j.isci.2024.110655. 
            eCollection 2024 Sep 20.
  PUBMED    39252965
REFERENCE   2  (bases 1 to 9020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-08-8"; volume: "27(9):110655. doi"; pages: " 
            10.1016/j.isci.2024.110655. eCollection 2024 Sep 20"
COMMENT     Sequence Label: pLenti_CMV:Flag-OeNL
FEATURES             Location/Qualifiers
     source          1..9020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     178..195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    312..333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(348..353,354..371,372..378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    386..402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     391..413
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     410..426
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     410..426
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     445..465
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        447..465
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        493..719
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             720..900
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    947..1072
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1565..1798
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1983..2027
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2294..2410
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2433..2736
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2737..2940
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2891..2911
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2937..2961
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3020..3043
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3281..3931
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             3281..3931
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     primer_bind     complement(4346..4366)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4351..4680
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4531..4666
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4690..5289
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4690..4709)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5186..5206
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5356..5944
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5409..5429)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5827..5838)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6031..6264
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6336..6457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6373..6392)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6427..6446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6497..6632
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6652..6671)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6653..6671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6681..6698)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6681..6697)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6690..6712)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6839..7294
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6926..6945)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7136..7157
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7320..7424
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7425..7493,7494..8285)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7643..7662)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8456..9020,1..24)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8945..8964
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
       61 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      121 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      181 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
      241 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      301 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
      361 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
      421 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
      481 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
      541 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
      601 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
      661 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
      721 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      781 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      841 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      901 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      961 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1021 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1081 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1141 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1201 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1261 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1321 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1381 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     1441 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1501 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1561 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1621 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1681 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1741 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1801 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1861 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1921 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1981 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2041 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2101 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2161 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2221 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     2281 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2341 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat
     2401 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
     2461 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     2521 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     2581 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     2641 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     2701 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     2761 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     2821 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     2881 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2941 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     3001 agacaccgac tctagaatgg actacaagga cgacgatgac aaggtcttca cactcgaaga
     3061 tttcgttggg gactggcgac agacagccgg ctacaacctg gaccaagtcc ttgaacaggg
     3121 aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta actccgatcc aaaggattgt
     3181 cctgagcggt ctcgagggcg gtagcggtgg cagcgtgagc gtgatcaagc ccgagatgaa
     3241 gaaggtggag gacgccgtgg cccactccac tctcgagggc gtgagcgtga tcaagcccga
     3301 gatgaagatg aggtactaca tggacggctc cgtcaatggg catgagttca caatcgaggg
     3361 tgagggcaca ggcagacctt acgagggaca tcaggagatg acactgcgcg tcacaatggc
     3421 cgagggcggg ccaatgcctt tcgccttcga cctggtgtcc cacgtgttct gttacggcca
     3481 cagagttttt actaaatatc cagaagagat cccagactat ttcaagcagg cctttcctga
     3541 gggcctgtcc tgggagaggt ccctggagtt cgaggacggc ggctccgcct ccgtgagcgc
     3601 ccacatcagc ctgaggggca acaccttcta ccacaagtcc aagttcaccg gcgtgaactt
     3661 ccccgccgac ggccccatca tgcagaacca gagcgtggac tgggagccct ccaccgagaa
     3721 gatcaccgcc agcgacggcg tgctgaaggg cgacgtgacc atgtacctga agctggaggg
     3781 cggcggcaac cacaagtgcc agatgaagac cacctacaag gccgccaagg agatcctgga
     3841 gatgcccggc gaccactaca tcggccacag gctggtgagg aagaccgagg gcaacatcac
     3901 cgagcaggtg gaggacgccg tggcccactc cgagctcgaa aatgggctga agatcgacat
     3961 ccatgtcatc atcccgtatg aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat
     4021 ttttaaggtg gtgtaccctg tggatgatca tcactttaag gtgatcctgc actatggcac
     4081 actggtaatc gacggggtta cgccgaacat gatcgactat ttcggacggc cgtatgaagg
     4141 catcgccgtg ttcgacggca aaaagatcac tgtaacaggg accctgtgga acggcaacaa
     4201 aattatcgac gagcgcctga tcaaccccga cggctccctg ctgttccgag taaccatcaa
     4261 cggagtgacc ggctggcggc tgtgcgaacg cattctggcg taaggatccg gactagttaa
     4321 cgcgtcgacc agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4381 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4441 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4501 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4561 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4621 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4681 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     4741 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     4801 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     4861 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     4921 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     4981 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     5041 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     5101 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     5161 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     5221 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     5281 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     5341 gcatcgccag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5401 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5461 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5521 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5581 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5641 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5701 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     5761 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5821 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5881 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5941 ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6001 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     6061 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6121 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6181 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6241 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     6301 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     6361 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6421 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     6481 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6541 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6601 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     6661 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6721 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6781 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     6841 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6901 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6961 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7021 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7081 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7141 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7201 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7261 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     7321 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7381 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7441 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7501 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7561 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7621 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7681 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7741 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7801 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7861 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7921 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7981 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8041 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8101 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8161 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8221 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8281 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8341 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8401 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8521 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8581 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8641 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8701 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8761 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8821 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8881 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8941 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9001 gtcgattttt gtgatgctcg
//
