LOCUS       sequence-424346-        9310 bp    DNA     circular SYN 15-APR-2025
DEFINITION  GFP fluorescent reporter for insulin gene expression. IRES-EGFP and 
            PuroR with homology arms for human insulin gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9310)
  TITLE     INS-IRES-GFP plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: INS-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..9310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      96..684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     585..604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     838..855
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    972..993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1008..1013,1014..1031,1032..1038)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1046..1062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1051..1073
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1070..1086
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1070..1086
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1107..1125
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2158..2710
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2302..2319)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2529..2548
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2722..2724,2725..2727,2728..3441)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2722..2724,2725..2727,2728..3438)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2767..2788)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3028..3047)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3375..3396
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3508..3541
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3568..4067
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3603..3626)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4000..4019
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4107..4706
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4107..4126)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4603..4623
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    5253..5286
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5293..5517
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6547..6566)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6548..6566)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6573..6590)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6573..6589)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6582..6604)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6727..7029
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6908..6927
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             7378..8172
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7378..8172
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7432..7451)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8042..8061
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(8422..9213,9214..9282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9045..9064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atttatcagg gttattgtct catgaccaaa atcccttaac gtgagttttc gttccactga
       61 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      121 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      181 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      241 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      301 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      361 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      421 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      481 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      541 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      601 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      661 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      721 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      781 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      841 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
      901 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      961 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     1021 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     1081 tatgaccatg attacgccaa gctcagaatt aaccctcact aaagggacta gtcctgcagg
     1141 ctacctagtg tgcggggaac gaggcttctt ctacacaccc aagacccgcc gggaggcaga
     1201 ggacctgcag ggtgagccaa ccgcccattg ctgcccctgg ccgcccccag ccaccccctg
     1261 ctcctggcgc tcccacccag catgggcaga agggggcagg aggctgccac ccagcagggg
     1321 gtcaggtgca cttttttaaa aagaagttct cttggtcacg tcctaaaagt gaccagctcc
     1381 ctgtggccca gtcagaatct cagcctgagg acggtgttgg cttcggcagc cccgagatac
     1441 atcagagggt gggcacgctc ctccctccac tcgcccctca aacaaatgcc ccgcagccca
     1501 tttctccacc ctcatttgat gaccgcagat tcaagtgttt tgttaagtaa agtcctgggt
     1561 gacctggggt cacagggtgc cccacgctgc ctgcctctgg gcgaacaccc catcacgccc
     1621 ggaggagggc gtggctgcct gcctgagtgg gccagacccc tgtcgccagc ctcacggcag
     1681 ctccatagtc aggagatggg gaagatgctg gggacaggcc ctggggagaa gtactgggat
     1741 cacctgttca ggctcccact gtgacgctgc cccggggcgg gggaaggagg tgggacatgt
     1801 gggcgttggg gcctgtaggt ccacacccag tgtgggtgac cctccctcta acctgggtcc
     1861 agcccggctg gagatgggtg ggagtgcgac ctagggctgg cgggcaggcg ggcactgtgt
     1921 ctccctgact gtgtcctcct gtgtccctct gcctcgccgc tgttccggaa cctgctctgc
     1981 gcggcacgtc ctggcagtgg ggcaggtgga gctgggcggg ggccctggtg caggcagcct
     2041 gcagcccttg gccctggagg ggtccctgca gaagcgtggc attgtggaac aatgctgtac
     2101 cagcatctgc tccctctatc agctggagaa ctactgcaac tgaggcgcgc ccccctaacg
     2161 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     2221 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     2281 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     2341 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     2401 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     2461 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     2521 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     2581 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     2641 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     2701 gatgataata tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2761 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2821 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2881 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2941 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3001 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3061 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3121 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     3181 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     3241 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     3301 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     3361 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3421 atggacgagc tgtacaagta aggatccacg cgtggcaata aaaagacaga ataaaacgca
     3481 cgggtgttgg gtcgtttgtt cgctagcata acttcgtata atgtatgcta tacgaagtta
     3541 tgaattcgtc gacctcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     3601 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     3661 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     3721 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     3781 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     3841 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     3901 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     3961 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     4021 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc
     4081 tagatctcga gcagctgaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     4141 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     4201 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     4261 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     4321 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     4381 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     4441 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     4501 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     4561 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     4621 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     4681 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     4741 ggagcgcacg accccatgca tcgatgatat cagatccccg ggatgcagaa attgatgatc
     4801 tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa
     4861 gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg
     4921 gggtgggatt agataaatgc ctgctcttta ctgaaggctc tttactattg ctttatgata
     4981 atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat
     5041 tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg
     5101 attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa
     5161 gaacgagatc agcagcctct gttccacata cacttcattc tcagtattgt tttgccaagt
     5221 tctaattcca tcagaagctg gtcgactcta gcataacttc gtataatgta tgctatacga
     5281 agttattcta gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     5341 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     5401 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     5461 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatgggcg
     5521 gccgctagac gcagcctgca ggcagcccca cacccgccgc ctcctgcacc gagagagatg
     5581 gaataaagcc cttgaaccag ccctgctgtg ccgtctgtgt gtcttggggg ccctgggcca
     5641 agccccactt cccggcactg ttgtgagccc ctcccagctc tctccacgct ctctgggtgc
     5701 ccacaggtgc caacgccggc caggcccagc atgcagtggc tctccccaaa gcggccatgc
     5761 ctgtcggctg cctgctgccc ccaccctgtg gctcagggtc cagtatggga gctgcggggg
     5821 tctctgaggg gccaggggtg gtggggccac tgagaagtga cttcttgttc agtagctctg
     5881 gactcttgga gtccccagag accttgttca ggaaagggaa tgagaacatt ccagcaattt
     5941 tccccccacc tagccctccc aggttctatt tttagagtta tttctgatgg agtccctgtg
     6001 gagggaggag gctgggctga gggagggggt cctgcagggc ggggggctgg gaaggtgggg
     6061 agaggctgcc gagagccacc cgctatcccc agctctgggc agccccggga cagtcacaca
     6121 ccctggcctc gcggcccaag ctggcagccg tctgcagcca cagcttatgc cagcccaggt
     6181 ccagccagac acctgaggga cccactggtg ccttggagga agcaggagag gtcagatggc
     6241 accatgagct ggggcaggtg cagggaccgt ggcagcacct ggcagggcct cagaacccat
     6301 gccttgggca ccccggccat gaggccctga ggattgcagc ccaggagaag cagggaaccg
     6361 ccagggccac aggggcagag accagggcca gggtccccct gcagcccctt agcccacccc
     6421 ctcccagtaa gcagggctgc ttggctggct tcctttgcta cagacctgct gctcacccag
     6481 aagggcccac gggccctggt gacaaggtcg ttgtggctcc aggtcgcggc cgctaaattc
     6541 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     6601 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6661 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctatac
     6721 gtacggcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     6781 gtacagagtg atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca
     6841 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     6901 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     6961 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     7021 ggaatataaa tgtcaggcat gagattatca aaaaggatct tcacctagat ccttttcacg
     7081 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
     7141 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
     7201 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
     7261 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
     7321 atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg
     7381 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     7441 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     7501 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     7561 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     7621 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     7681 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     7741 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     7801 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     7861 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     7921 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     7981 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     8041 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     8101 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     8161 gagttcttct gaattattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
     8221 ctgtgcggta tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     8281 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagat tatcaaaaag
     8341 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8401 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8461 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8521 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8581 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8641 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8701 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8761 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8821 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8881 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8941 gccatccgta agatgctttt ctgtgactgg tgaatactca accaagtcat tctgagaata
     9001 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9061 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9121 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9181 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9241 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9301 ttattgaagc
//
