LOCUS       sequence-424516-       11065 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Lindsey-Boltz LA, Sancar A
  TITLE     Tethering DNA Damage Checkpoint Mediator Proteins Topoisomerase 
            II{beta}-binding Protein 1 (TopBP1) and Claspin to DNA Activates 
            Ataxia-Telangiectasia Mutated and RAD3-related (ATR) Phosphorylation
            of Checkpoint Kinase 1 (Chk1).
  JOURNAL   J Biol Chem. 2011 Jun 3. 286(22):19229-36.
  PUBMED    21502314
REFERENCE   2  (bases 1 to 11065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 Jun 3. 286(22):19229-36."
COMMENT     Sequence Label: pcDNA3-LacR-TopBP1
FEATURES             Location/Qualifiers
     source          1..11065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..52)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        224..603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        604..807
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     758..778
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     852..871
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        852..870
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(903..922)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             1949..1969
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1982..2005
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(5625..5636)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(6607..6625)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6608..6625)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6645..6662)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6651..6875
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6921..7349
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7008..7027)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7218..7239
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7358..7378)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7363..7692
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7543..7678
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7605..7624
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7759..8553
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7813..7832)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8423..8442
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8727..8848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8764..8783)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8818..8837
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8897..8913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8897..8913)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8910..8932)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8921..8937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8945..8951,8952..8969,8970..8975))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8990..9011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9128..9145)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9299..9887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9379..9398)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10058..10849,10850..10918))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10681..10700
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10919..11023)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
       61 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
      121 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
      181 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
      241 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      301 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      361 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      421 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      481 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      541 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      601 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      661 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      721 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      781 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
      841 cttatcgaaa ttaatacgac tcactatagg gagacccaag cttaccatgc cagtaacgtt
      901 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
      961 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     1021 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     1081 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     1141 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     1201 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     1261 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     1321 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     1381 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     1441 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     1501 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     1561 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     1621 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     1681 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     1741 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     1801 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     1861 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1921 aatgcagctg gcacgacagg tttcccgacc caagaagaag aggaaggtgg gatccaccat
     1981 ggattacaaa gacgatgacg acaagtccag aaatgacaaa gaaccgtttt ttgtgaagtt
     2041 tttaaagtct tcagacaatt ccaaatgttt ttttaaagct ctcgagtcca taaaagaatt
     2101 ccaatcagaa gaatatcttc agattattac agaagaagag gcattgaaga taaaggagaa
     2161 tgatagatca ctttatatct gtgacccttt tagtggcgtt gtctttgatc acctcaaaaa
     2221 gcttggctgc agaattgttg gtcctcaagt agtcatattt tgtatgcacc accagcgatg
     2281 tgtcccaaga gccgaacatc cagtttataa tatggttatg tctgatgtaa ccatatcttg
     2341 tacaagtctg gaaaaagaaa aaagggaaga agttcataaa tatgtacaaa tgatgggcgg
     2401 acgagtatac agagacctta atgtatcagt aactcacctt attgcaggag aagttggtag
     2461 caaaaaatat ttagttgctg caaacctgaa gaaacctatt ttgcttccct cttggataaa
     2521 aacactttgg gagaagtcac aagagaaaaa aataactaga tatactgata taaacatgga
     2581 agatttcaag tgtcctattt ttcttggttg cataatctgt gtgactggct tatgtggctt
     2641 agacaggaaa gaagttcagc aactcacagt taagcatgga ggtcaataca tgggacaatt
     2701 gaaaatgaat gaatgtacac acctcattgt gcaagaacca aaaggtcaga agtatgagtg
     2761 tgccaagaga tggaatgtac actgtgtgac cacacagtgg ttttttgaca gtattgagaa
     2821 aggtttttgt caggatgaat ccatatacaa gacagaacct agaccagaag caaagactat
     2881 gcccaattct tcaactccta ccagccagat caacacaatt gatagtcgta ctctttcaga
     2941 tgtcagcaat atttccaaca taaatgcaag ttgcgtaagt gaatcaatat gtaattcact
     3001 taacagcaaa ctggagccta cacttgaaaa tctagaaaat ctggatgtca gtgcatttca
     3061 agcacctgaa gatttattag atggttgtcg gatatatctt tgcggtttta gtggcagaaa
     3121 gctagataaa ctgagaagac ttattaacag tggaggtgga gttcgtttta accagctaaa
     3181 tgaagatgta actcatgtta ttgtgggaga ttatgatgat gaattgaagc agttttggaa
     3241 taaatcagcc cacaggcctc atgtagtggg agcaaagtgg ttgctagagt gtttcagtaa
     3301 aggttatatg ctttctgaag aaccatatat ccatgctaat taccagccag tggaaattcc
     3361 agtttcacat cagcctgaaa gtaaagcagc tcttttaaaa aagaagaaca gcagcttctc
     3421 taagaaagac tttgctccta gtgaaaagca tgagcaagct gatgaagatc tgctctctca
     3481 atatgaaaat ggtagctcca cagtagttga ggctaagacg tctgaagcca ggccctttaa
     3541 tgattctact catgctgagc ccttgaatga ttctactcac atttctttgc aagaagaaaa
     3601 ccagtcttct gtcagtcatt gtgtccctga tgtttctaca attactgaag aaggcttatt
     3661 tagccaaaag agtttccttg ttttgggttt tagtaatgaa aatgaatcta acatcgcaaa
     3721 catcataaaa gaaaatgctg ggaaaatcat gtcccttctg agcagaactg ttgcggatta
     3781 tgctgtggtt cctctgctgg ggtgtgaagt ggaagccact gtgggagaag ttgttacaaa
     3841 tacatggctg gttacttgca tagactatca gactttgttt gatccaaagt cgaatcctct
     3901 cttcacacca gttccagtaa tgacaggaat gactccttta gaggattgtg ttatttcatt
     3961 tagccagtgt gctggagcag aaaaagagtc tttaacattc ctagcaaacc tccttggagc
     4021 aagtgttcaa gaatactttg ttcgcaaatc caatgcaaag aaaggcatgt ttgccagtac
     4081 tcatcttata ctgaaagaac gtggtggctc taaatatgaa gctgcaaaga agtggaattt
     4141 acctgccgtt actatagctt ggctgttgga gactgctaga acgggaaaga gagcagacga
     4201 aagccatttt ctgattgaaa attcaactaa agaagaacga agtttggaaa cagaaataac
     4261 aaatggaatc aatctaaatt cagatactgc agagcatcct ggcacacgcc tgcaaactca
     4321 cagaaaaacc gtcgttacac ctttagatat gaaccgcttt cagagtaaag ctttccgtgc
     4381 tgtggtctca caacatgcca gacaggtcgc agcctcccca gcagtaggac aaccacttca
     4441 gaaggagccc tcgttacacc tggatacacc atcaaaattc ctgtccaagg acaaactctt
     4501 caagccttcc tttgatgtga aggatgcact tgcagccttg gaaactccag gacgtcccag
     4561 ccaacagaaa aggaaaccga gtacgccact ctcagaagtt attgtcaaaa acttgcaact
     4621 tgctttggca aatagctctc gaaatgctgt cgctctttct gccagccctc aactgaaaga
     4681 ggcccagtca gagaaggaag aagccccaaa gccacttcac aaagtagtgg tatgtgttag
     4741 taaaaaactc agtaagaagc agagtgaact aaatgggatc gcagcctctc taggagcaga
     4801 ttacaggtgg agttttgatg aaacagtgac tcatttcatc tatcaagggc ggccaaatga
     4861 cactaatcgg gagtataaat ctgtaaaaga aagaggagta cacattgttt ccgagcactg
     4921 gcttttagat tgtgcccaag agtgtaaaca tcttcctgaa tctctttatc cacatactta
     4981 taatcccaaa atgagcttgg atatcagcgc agtgcaagat ggccggctct gtaatagtcg
     5041 actactctca gctgtgtctt caacaaagga tgatgagcca gatcctttga ttttagaaga
     5101 aaatgatgta gacaatatgg ccaccaataa taaagagtca gcaccatcaa atggaagtgg
     5161 aaagaatgac tctaaaggag ttctgacaca gaccttagag atgagagaga actttcagaa
     5221 gcagttacag gagataatgt ctgcaacatc aatagtgaaa ccccaagggc agaggacttc
     5281 cctttcaaga agtggttgta acagcgcatc ttcaacccct gacagcactc gctctgctcg
     5341 cagtggacga agtagagtcc tagaggcact gaggcagtct cgtcagacag tacctgatgt
     5401 caacacagag ccttcccaaa atgaacagat catttgggat gaccctacag caagggagga
     5461 gagagcaagg cttgccagca atttgcagtg gcctagttgt cccacacaat actctgagct
     5521 tcaggttgac attcaaaact tggaggattc tccttttcaa aagcctttac atgattcaga
     5581 aattgctaaa caggctgtct gtgatcctgg aaacatacgt gtgactgaag ctcccaaaca
     5641 cccaatctct gaagaactgg aaactcccat aaaagacagc cacctgatcc ctacgcctca
     5701 agcccccagt attgcctttc cactcgccaa cccccctgtg gctccgcacc ctagagaaaa
     5761 gattataacg atagaggaga ctcatgaaga attaaaaaaa cagtacatat ttcagttatc
     5821 atctctgaat cctcaagaac gtattgacta ttgtcatctg attgagaaac taggtggatt
     5881 ggtgatagaa aagcagtgct ttgatcccac ctgtacacac attgttgtgg gacatccact
     5941 tcgaaacgag aagtatttag cctcagtggc agctgggaag tgggtgcttc atcgctccta
     6001 ccttgaagcc tgcaggactg ctggacactt cgtgcaggaa gaagactatg aatggggaag
     6061 tagttccata cttgatgttt tgactggaat caatgtacag caacgaagac tagcacttgc
     6121 agcaatgaga tggagaaaaa aaatccagca aagacaagaa tctggcattg ttgagggagc
     6181 atttagtggg tggaaggtta ttttacatgt ggatcagtct cgagaagcag gcttcaaacg
     6241 ccttcttcag tcaggaggag caaaggtgct acctggtcat tctgtacctt tatttaaaga
     6301 ggccacacat cttttttctg acttgaataa actgaaacca gatgactcag gagttaatat
     6361 agcagaagct gctgcccaga acgtgtactg cttgagaaca gaatacattg ctgattatct
     6421 catgcaggaa tcacctcctc atgtagaaaa ttactgtcta ccagaagcta tttcatttat
     6481 tcagaataat aaggaacttg ggactggatt atcacaaaag aggaaagctc ctacagaaaa
     6541 aaataaaatc aaacgaccta gagtacacta agcggccgcg tcgagcatgc atctagaggg
     6601 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc
     6661 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6721 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6781 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6841 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     6901 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6961 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7021 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7081 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7141 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7201 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7261 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7321 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     7381 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7441 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7501 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7561 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7621 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7681 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     7741 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     7801 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     7861 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     7921 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     7981 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     8041 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     8101 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     8161 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     8221 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     8281 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     8341 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     8401 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     8461 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     8521 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     8581 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     8641 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     8701 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     8761 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8821 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     8881 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8941 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9001 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9061 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9721 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9961 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10021 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10081 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10141 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10201 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10261 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10321 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10381 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10441 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10501 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10561 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10621 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10681 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10741 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10801 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10861 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10921 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10981 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11041 tgccacctga cgtcgacgga tcggg
//
