LOCUS       sequence-424633-        6580 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Transient expression of STING R232.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Dobbs N, Burnaevskiy N, Chen D, Gonugunta VK, Alto NM, Yan N
  TITLE     STING Activation by Translocation from the ER Is Associated with 
            Infection and Autoinflammatory Disease.
  JOURNAL   Cell Host Microbe. 2015 Aug 12;18(2):157-68. doi: 
            10.1016/j.chom.2015.07.001. Epub 2015 Jul 30.
  PUBMED    26235147
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2015.07.001"; journalName: "Cell Host Microbe"; 
            date: "2015-08-12- 12"; volume: "18"; issue: "2"; pages: "157-68"
COMMENT     Sequence Label: pcDNA3.1-hSTING (R232)
FEATURES             Location/Qualifiers
     source          1..6580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      196..784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     685..704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     938..955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1072..1093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1108..1113,1114..1131,1132..1138)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1146..1162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1151..1173
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1170..1186
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1170..1186
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1235..1356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1246..1265)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1300..1319
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1530..2324)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1641..1660)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2251..2270
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2391..2720)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2405..2540
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2459..2478)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2705..2725
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2734..3162)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2844..2865)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3056..3075
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3208..3432
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3421..3438
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(4727..4746)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4728..4746)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4791..4994)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4820..4840)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4995..5374
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5546..5565
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5640..5744
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5745..5813,5814..6580,1..25)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5963..5982)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
       61 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
      121 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
      181 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
      241 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      301 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
      361 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      421 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      481 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      541 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      601 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      661 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      721 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      781 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      841 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      901 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      961 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1021 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     1081 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1141 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1201 gctctagcta gaggtcgacg gtatacagac atgataagat acattgatga gtttggacaa
     1261 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     1321 ttatttgtaa ccattataag ctgcaataaa caagttgggg tgggcgaaga actccagcat
     1381 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     1441 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     1501 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     1561 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     1621 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     1681 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     1741 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc
     1801 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     1861 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     1921 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     1981 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     2041 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     2101 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca
     2161 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     2221 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     2281 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     2341 cctgtctctt gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag
     2401 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc
     2461 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     2521 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     2581 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     2641 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     2701 ccacacccta actgacacac attccacaga attaattcgc gttaaatttt tgttaaatca
     2761 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2821 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2881 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2941 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3001 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3061 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3121 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc
     3181 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
     3241 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     3301 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     3361 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     3421 tagaaggcac agtcgaggct gatcagcggg tttaaacggg ccctctagac tcgagcggcc
     3481 gccactgtgc tggatatctg cagaattctc aagagaaatc cgtgcggaga gggaggggct
     3541 tttccattcc actgatgagg agctcaggct cttgggacat cgtggaggta ctgggcaccg
     3601 ctgaggtctt caagctgccc acagtaacct cttccttttc ctcctgccgc aggtgccgga
     3661 gaacctcctg ggacagcgag aagctgctgt catctgcagg ttcctggtag gcaatgaggc
     3721 ggcagttgtt ctgagactca ggggcatctg ccaggatgtc ctcaagtgtc cggcagaaga
     3781 gtttggcctg ctcaagccta tcctcccggc taaagccagc ttgactgtat tgtgacatgg
     3841 caaacaaagt ctgcaagggg gtggcgtact ccaggacaca ggtgcccgcc cgctgcccgt
     3901 tctccagaag ctcatagatg ctgttgctgt aaacccgatc cttgatgcca gcacggtcac
     3961 cggtctgctg gggcagttta tccaggaagc gaatgttggg gtcagccata ctcaggttat
     4021 caggcacccc acagtccaat gggaggagaa tatacagccg ctggctcact gcaccccgta
     4081 gcaggttgtt gtaatgctga ttgtaagttc gaatccgggc ctggagctct ggcaggatca
     4141 gccgcagata tccgatgtaa tatgaccatg ccagcccatg ggccacgttg aaattccctt
     4201 tttcacacac tgcagagatc tcagctgggg ccaggccctt gaggcccagg aggatgttca
     4261 gtgcctgcga gaggcccagg agggcaagca tccaagtgaa gggcgggccg accgcatttg
     4321 ggagggagta gtagaaatag atggacagca gcaacagggc cccacggcgg agggggcagc
     4381 ccaggcaggc ccgcacagtc ctccagtagc tgccccggta cctggagtgg atgtggcgca
     4441 gctcctcagc caggctgcag accccgttta acagcagtcc cagctgcagg gaggctaggt
     4501 ggagcaccag gtaccggaga gtgtgctctg gtggctctcc tagcccccaa agggtcacca
     4561 ggcaggcact cagcagaacc aaggctgcct tctgggcccc gtgacccctg ggacacggga
     4621 tggatggatg caggctggag tggggcatgg tgaattccac cacactggac tagtggatcc
     4681 gagctcggta ccaagcttaa gtttaaacgc tagccagctt gggtctccct atagtgagtc
     4741 gtattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt
     4801 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     4861 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     4921 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     4981 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     5041 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     5101 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     5161 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     5221 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     5281 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     5341 gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg
     5401 cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct
     5461 tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca
     5521 gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     5581 aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc
     5641 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5701 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5761 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5821 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5881 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5941 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6001 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6061 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6121 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6181 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6241 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6301 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6361 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6421 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6481 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6541 caactatgga tgaacgaaat agacagatcg ctgagatagg
//
