LOCUS       sequence-424750-        8032 bp    DNA     circular SYN 15-APR-2025
DEFINITION  10xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8032)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS401_myc-10xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..8032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    108..129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(144..149,150..167,168..174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    182..198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     187..209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     206..222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     206..222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     241..261
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        243..261
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        289..515
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             516..696
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    743..868
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1365..1598
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1783..1827
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2005..2025
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2226..2343
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2415..2626
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2655..2684
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2727..2747
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2760..2804
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             2841..2906
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2922..2987
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3003..3068
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3084..3149
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3165..3230
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3246..3311
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3327..3392
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3408..3473
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3489..3554
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3570..3635
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3669..3722
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3729..4127
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4173..4761
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4226..4246)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4644..4655)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4833..5066
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5144..5265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5181..5200)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5235..5254
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5305..5440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5460..5479)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5461..5479)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5489..5506)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5489..5505)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5498..5520)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5647..6102
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5734..5753)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5944..5965
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6128..6232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6233..6301,6302..7093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6451..6470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7264..7852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7753..7772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8006..8023
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
       61 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      121 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      181 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      241 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      301 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      361 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      421 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      481 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      541 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      601 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      661 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      721 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      781 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      841 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
      901 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
      961 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1021 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1081 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1141 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1201 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1261 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1321 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1381 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1441 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1501 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1561 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1621 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1681 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1741 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1801 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1861 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     1921 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     1981 ctcccaaccc cgaggggacc cagagagggc ctatttccca tgattccttc atatttgcat
     2041 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
     2101 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     2161 ttatgtttta aaatggacta tcataaattc tagatcttga gacaaatggc agtattcatc
     2221 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2281 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2341 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcgt cgagtggctc
     2401 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     2461 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     2521 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     2581 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg
     2641 gatccgatat catggagcag aagctgatca gcgaggagga cctgggaggg gatcggtgga
     2701 gctccaccgg tggcggccgc tctagagaga atctttattt tcagggcgcc gccaaattca
     2761 aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga cagcggaggt ggaggtacta
     2821 gtctcgaggg ttctggagga ttgttgccca aaaactacca cttggagaac gaggtcgcaa
     2881 ggttgaaaaa attggtcggt gagcgtggcg gctcaggcgg ccttcttcca aaaaattatc
     2941 atcttgaaaa tgaagttgct agacttaaaa aacttgttgg agaaagaggc gggagcggcg
     3001 gcctgctgcc caagaactac cacctggaga acgaggtcgc ccgcctgaag aagctggtcg
     3061 gcgagcgcgg tggttctgga ggtttgctcc caaagaacta ccacttggaa aacgaagtcg
     3121 caagattgaa gaagttggtc ggtgaaagag gaggaagcgg aggattatta cctaaaaatt
     3181 atcatttgga aaatgaagtt gctagattga agaagttggt tggagagaga ggggggtctg
     3241 gaggactact accgaaaaac taccacctag agaacgaggt ggcccgacta aaaaagctag
     3301 tgggcgagcg cggcggcagt ggcggcctcc tccccaagaa ttaccacctc gaaaatgaag
     3361 tagcaaggct caagaagctc gtaggagaaa gaggtggttc cgggggactc cttccaaaaa
     3421 actatcatct tgaaaacgaa gttgcacgtc ttaaaaaact tgttggcgaa cgtggcggct
     3481 ctggcgggct gctgcccaag aattaccacc tggaaaatga agtggcccgc ctgaagaagc
     3541 tggtgggcga acgcggaggc agtggaggcc tactaccgaa gaactaccac ctagagaacg
     3601 aggtggcacg cctaaagaag ctagtgggcg agcgcggcgg ctcgggtggt ttattaccta
     3661 aggaattcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     3721 ctatcgatat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     3781 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     3841 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     3901 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     3961 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     4021 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     4081 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaaggt accactagtc
     4141 ccgggatgca tagcgctacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     4201 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4261 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4321 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4381 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4441 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4501 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4561 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4621 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4681 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4741 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     4801 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     4861 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     4921 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4981 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5041 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     5101 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     5161 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5221 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     5281 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5341 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5401 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     5461 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     5521 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     5581 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5641 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5701 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5761 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5821 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5881 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5941 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6001 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6061 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     6121 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6181 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6241 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6301 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6361 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6421 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6481 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6541 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6601 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6661 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6721 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6781 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6841 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6901 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6961 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7021 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7081 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7141 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7201 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7261 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7321 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7381 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7441 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7501 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7561 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7621 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7681 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7741 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7801 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7861 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7921 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7981 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aa
//
