LOCUS       sequence-424813-        8948 bp    DNA     circular SYN 15-APR-2025
DEFINITION  4xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8948)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 8948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS376_tdTomato-4xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..8948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    77..98
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(113..118,119..136,137..143)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    151..167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     156..178
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     175..191
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     175..191
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     210..230
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        212..230
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        258..484
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             485..665
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    712..837
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1334..1567
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1752..1796
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1974..1994
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2195..2312
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2384..2595
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2628..4055
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4101..4121
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4134..4178
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             4215..4280
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4296..4361
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4377..4442
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4458..4523
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4554..4607
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4614..5012
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5058..5646
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5111..5131)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5529..5540)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5718..5951
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6029..6150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6066..6085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6120..6139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6190..6325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6345..6364)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6346..6364)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6374..6391)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6374..6390)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6383..6405)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6532..6987
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6619..6638)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6829..6850
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7013..7117
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7118..7186,7187..7978)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7336..7355)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8149..8737
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8638..8657
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8891..8908
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
       61 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      121 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      181 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
      241 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
      301 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
      361 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
      421 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
      481 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      541 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      601 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      661 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      721 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      781 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      841 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      901 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      961 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1021 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1081 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1141 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
     1201 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1261 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1321 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1381 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1441 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1501 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1561 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1621 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1681 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1741 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1801 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1861 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1921 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc agagagggcc
     1981 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta
     2041 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     2101 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat cataaattct
     2161 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2221 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2281 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2341 cactttggcg ccggctcgtc gagtggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     2401 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     2461 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     2521 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     2581 gccgccagaa cacaggtgtc gtgacgcggg atccgatatc cgccaccatg gtgagcaagg
     2641 gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg
     2701 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2761 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
     2821 agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga
     2881 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc
     2941 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga
     3001 tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
     3061 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg
     3121 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca
     3181 agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac atcacctccc
     3241 acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt
     3301 tcctggggca tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg
     3361 acaacaacat ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca
     3421 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     3481 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     3541 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt
     3601 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     3661 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg
     3721 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg
     3781 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     3841 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca
     3901 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca
     3961 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc
     4021 acctgttcct gtacggcatg gacgagctgt acaagaccgg aggggatcgg tggagctcca
     4081 ccggtggcgg ccgctctaga gagaatcttt attttcaggg cgccgccaaa ttcaaagaaa
     4141 ccgctgctgc taaattcgaa cgccagcaca tggacagcgg aggtggaggt actagtctcg
     4201 agggttctgg aggattgttg cccaaaaact accacttgga gaacgaggtc gcaaggttga
     4261 aaaaattggt cggtgagcgt ggcggctcag gcggccttct tccaaaaaat tatcatcttg
     4321 aaaatgaagt tgctagactt aaaaaacttg ttggagaaag aggcgggagc ggcggcctgc
     4381 tgcccaagaa ctaccacctg gagaacgagg tcgcccgcct gaagaagctg gtcggcgagc
     4441 gcggtggttc tggaggtttg ctcccaaaga actaccactt ggaaaacgaa gtcgcaagat
     4501 tgaagaagtt ggtcggtgaa agaggaggaa gcggaggatt attacctgaa ttcgagggca
     4561 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatc gatatggcca
     4621 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     4681 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
     4741 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
     4801 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
     4861 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
     4921 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
     4981 tgctgccctc tggttatgtg tgggagggct aaggtaccac tagtcccggg atgcatagcg
     5041 ctacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5101 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5161 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5221 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5281 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5341 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5401 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     5461 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5521 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5581 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5641 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5701 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     5761 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5821 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5881 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5941 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     6001 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6121 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     6181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6301 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     6361 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     6421 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     6481 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     6541 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6601 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6661 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6721 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6781 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6841 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6901 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6961 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     7021 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7081 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7141 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7201 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7261 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7321 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7381 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7441 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7501 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7561 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7621 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7681 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7741 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7801 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7861 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7921 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7981 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8041 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8101 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8161 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8221 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8281 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8341 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8401 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8461 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8521 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8581 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8641 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8701 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8761 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8821 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8881 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8941 tctccccg
//
