LOCUS       sequence-424836-        2326 bp    DNA     circular SYN 15-APR-2025
DEFINITION  medium-copy plasmid for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdO2k
FEATURES             Location/Qualifiers
     source          1..2326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      37..582
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     483..502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     699..716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     849..865
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     849..865
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1188..1205)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1188..1204)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1406..2200
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1460..1479)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2070..2089
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2201..2220
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2201..2219
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2308..2326)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt
       61 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
      121 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
      181 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
      241 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
      361 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
      421 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
      481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
      541 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc
      601 ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt
      661 aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
      721 cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc
      781 cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca gtatatacac
      841 tccgctagca ggaaacagct atgaccatgc ccgggtaagt gactagcgga aacacagaaa
      901 aaagcccgca cctgacagtg cgggcttttt ttttcgacca aaggctccga agagccgagt
      961 gtcttcggtt cgagaccgaa ttcgcgagct ctcgctagcc agtttaaaca attgcggccg
     1021 cgtactagtc tatcgatttg gatccaccct gcaggaagtc tagaccgtcg acacggtctc
     1081 tgagagaaga cacgtcgctc ttcgacgtaa gtaactgact cggtaccaaa ttccagaaaa
     1141 gaggcctccc gaaagggggg ccttttttcg ttttggtcct aagcttaact ggccgtcgtt
     1201 ttacatagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc
     1261 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
     1321 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca
     1381 agagacagga tgacggtcgt ttcgcatgct tgaacaagat ggattgcacg caggttctcc
     1441 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     1501 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     1561 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     1621 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     1681 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     1741 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     1801 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     1861 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     1921 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     1981 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     2041 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     2101 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     2161 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga taatacgact cactataggg
     2221 taactaattg aggcgcgcct accaccgtca aaaaaaacgg cgctttttag cgccgttttt
     2281 atttttcaac cttcctaggt aagtgaccct ttagtgaggg ttaatt
//
