LOCUS       sequence-424963-       11203 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF 
            with a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11203)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-FLAG-hMYOD1 (#800)
FEATURES             Location/Qualifiers
     source          1..11203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(72..80,81..116,117..119,120..155,156..158,
                     159..194,195..200,201..818))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(877..2088)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(1021..1040)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(2174..2200)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2253..2277
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3272..3295)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3295..3304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3325..3349)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3458..3482)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3479..3517)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(3550..3568,3569..3591,3592..3610,3611..3633,3634..3652,
                     3653..3677,3678..3696,3697..3719,3720..3738,3739..3761,
                     3762..3780,3781..3803,3804..3822)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_feature    3877..3994
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4287..4331)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4516..4749
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5246..5371
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5418..6051
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6243..6262)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6312..6328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6312..6328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6325..6347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6336..6352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6360..6366,6367..6384,6385..6390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6405..6426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6666..6683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6837..7425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6917..6936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7596..8387,8388..8456))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8219..8238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8457..8561)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8629..8647
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             8766..8999
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    9074..9662
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9180..9191
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9589..9609
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9839..10633)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(9839..10633)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9950..9969)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10560..10579
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    join(10649..11203,1..19)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10809..10828)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     11038..11055
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ggggggggag ggagaggggg gggggggaga ggggcggaat tcctctagtg cggccgcgga
       61 tccttactta gttacccggg gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc
      121 agcatatcaa ggtcaaagtc gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg
      181 tcaagggcgt cggtcggccc gccgctttcg cactttagct gtttctccag gccacatatg
      241 attagttcca ggccgaaaag gaaggcaggt tcggctccct gccggtcgaa cagctcaatt
      301 gcttgtctca gaagtggggg catagaatcg gtggtaggtg tctctctttc ctcttttgct
      361 acttgatgct cctgttcctc caatacgcag cccagtgtaa agtggcccac ggcggacaga
      421 gcgtacagtg cgttctccag ggagaagcct tgctgacaca ggaacgcgag ctgattttcc
      481 agggtttcgt actgtttctc tgttgggcgg gtgccgagat gcactttagc cccgtcgcga
      541 tgtgagagga gagcacagcg gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac
      601 tcgccttcca gggggcagaa gtgggtatga tgcctgtcca gcatctcgat tggcagggca
      661 tcgagcaggg cccgcttgtt cttcacgtgc cagtacaggg taggctgctc aactcccagc
      721 ttttgagcga gtttccttgt cgtcaggcct tcgataccga caccattgag taattccaga
      781 gctccgttta tgactttgct cttgtccagt ctagacatgg tgaattcgac tgcaggaccg
      841 gtacggggtg gagatccgag ctcggtacca agcttcgtct aacaaaaaag ccaaaaacgg
      901 ccagaattta gcggacaatt tactagtcta acactgaaaa ttacatattg acccaaatga
      961 ttacatttca aaaggtgcct aaaaaacttc acaaaacaca ctcgccaacc ccgagcgcat
     1021 agttcaaaac cggagcttca gctacttaag aagataggta cataaaaccg accaaagaaa
     1081 ctgacgcctc acttatccct cccctcacca gaggtccggc gcctgtcgat tcaggagagc
     1141 ctaccctagg cccgaaccct gcgtcctgcg acggagaaaa gcctaccgca cacctaccgg
     1201 caggtggccc caccctgcat tataagccaa cagaacgggt gacgtcacga cacgacgagg
     1261 gcgcgcgctc ccaaaggtac gggtgcactg cccaacggca ccgccataac tgccgccccc
     1321 gcaacagacg acaaaccgag ttctccagtc agtgacaaac ttcacgtcag ggtccccaga
     1381 tggtgcccca gcccatctca cccgaataag agctttcccg cattagcgaa ggcctcaaga
     1441 ccttgggttc ttgccgccca ccatgccccc caccttgttt caacgacctc acagcccgcc
     1501 tcacaagcgt cttccattca agactcggga acagccgcca ttttgctgcg ctccccccaa
     1561 cccccagttc agggcaacct tgctcgcgga cccagactac agcccttggc ggtctctcca
     1621 cacgcttccg tcccaccgag cggcccggcg gccacgaaag ccccggccag cccagcagcc
     1681 cgctactcac caagtgacga tcacagcgat ccacaaacaa gaaccgcgac ccaaatcccg
     1741 gctgcgacgg aactagctgt gccacacccg gcgcgtcctt atataatcat cggcgttcac
     1801 cgccccacgg agatccctcc gcagaatcgc cgagaaggga ctacttttcc tcgcctgttc
     1861 cgctctctgg aaagaaaacc agtgccctag agtcacccaa gtcccgtcct aaaatgtcct
     1921 tctgctgata ctggggttct aaggccgagt cttatgagca gcgggccgct gtcctgagcg
     1981 tccgggcgga aggatcagga cgctcgctgc gcccttcgtc tgacgtggca gcgctcgccg
     2041 tgaggagggg ggcgcccgcg ggaggcgcca aaacccggcg cggaggccag atcttgggtg
     2101 ggttactcca gactgccttg ggaaaagcgc ctcccctacc cggtagaatt tctagtttaa
     2161 ttaatcatta ctaagcgtag tctgggacgt cgtatgggta ttcgaaccgc gggccctcta
     2221 gactcgagcg gccgccactg tgctggatat caaccacttt gtacaagaaa gctgggtcgg
     2281 cgcgcccacc ctttcagagc acctggtata tcgggttggg gttcgcaccc gcagggcact
     2341 gcggggcggc gtccggtgac tgggtggggt cgccgctgct ctctccctcg ctgggggcgg
     2401 cagcctcttg cctgcgcgga ggcgactcag aaggcacgtc cgccagcagg agggcgggcg
     2461 ccgcagggct ctcggtggag atgcgctcca cgatgctgga caggcagtct aggctcgaca
     2521 ccgccgcact cttcccgggc ctgggttcgc tgggcgcctc gttgtagtag gcgccttcgt
     2581 agcagttccg ccgccgggcg ccgctcgggg ggccgctgta gtccatcatg ccgtcggagc
     2641 agttggagcg cgggctggac gcgtcggagt cgccgctgta gtgctcgccg ccgcggcccg
     2701 ggggcagcgg gcccggcgca tagaaggcgg ctgcggcgcc agggggcgcg gcgtcctggt
     2761 cgcgcagcag agcctgcagg ccctcgatat agcggatggc gttgcgcagg atctccacct
     2821 tgggcaaccg ctggtttgga ttgctcgacg tgcagcgctt gagtgtctca aaggcctcat
     2881 ttactttgct caggcggcgc cgctcgcgca tggtggcggc cttgcggcgg tcggcgttgg
     2941 tggtcttgcg cttgcacgcc ttgcaggccc acagtaggca gcggcccgcc tggtggtgcc
     3001 cgctgggcgc gcgcacatgc tcgtcctcac gtgcgcccgg ggccgggtgc accgccgcgg
     3061 ggaagtgcga gtgctcttcg ggtttcagga gcgcgcccac gtgcatcagg cgcgggtcca
     3121 ggtcttcgaa gaagcgcagg tccggggagt cgaaacacgg gtcgtcatag aagtcgtccg
     3181 ttgtggcaaa ggagcagaga gagccgtcgg gggccgtcag gtctacgtcg cggagcggtg
     3241 gcgacagtag ctccatggat ccgcccgggc tcttatcgtc gtcatccttg taatccatgg
     3301 tggcggtgaa gggggcggcc gcggagcctg cttttttgta caaacttgtt gatatctgca
     3361 gaattccacc acactggact agttcggggc cgcggaggct ggatcggtcc cggtgtcttc
     3421 tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag
     3481 ctctgcttat ataggcctcc caccgtacac gcctacctcg acccgggtac cgagctcgac
     3541 tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca
     3601 ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa
     3661 cactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
     3721 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
     3781 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ggatcctagt
     3841 ccaaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg
     3901 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
     3961 actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtga
     4021 attcggcgat accgtcgaga tccgttcact aatcgaatgg atctgtctct gtctctctct
     4081 ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc
     4141 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
     4201 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
     4261 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
     4321 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
     4381 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
     4441 gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag
     4501 caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt
     4561 gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata
     4621 gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc
     4681 gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac
     4741 aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg
     4801 ggtgctactc ctaatggttc aatttttact actttatatt tatataattc acttctccaa
     4861 ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccggccgct tgctgtgcgg
     4921 tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta
     4981 tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt
     5041 cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg
     5101 atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta
     5161 tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg
     5221 atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc
     5281 cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg
     5341 cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt
     5401 ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct
     5461 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
     5521 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
     5581 gtctaaccag agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg
     5641 ctcgccactc cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc
     5701 ccttgtagca agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca
     5761 cgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat
     5821 ccatcccatg caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt
     5881 ctaccttatc tggctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc
     5941 tgacccctgg ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga
     6001 tccacagatc aaggatatct tgtcttcttt gggagtgaat tagcccttcc aactactaag
     6061 tttgtagtac atatttaaca aatacaattt ctttaaaatg aaaataattc agaggaatca
     6121 caggtttaga gtaaatgaaa ccacaggtaa ttggcagtgg taatagggta tggggtggga
     6181 agtttgggat gattttggtt agcttgagtt atccagttga tccagacatg ataagataca
     6241 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     6301 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccacggg
     6481 gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     6541 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catgagcttg
     6601 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6781 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6841 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6901 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7081 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7141 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7201 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7261 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7321 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7381 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7441 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7501 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7561 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7621 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7681 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7741 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7801 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7861 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7921 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7981 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8041 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8101 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8161 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     8221 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8281 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8341 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8401 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8461 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8521 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8581 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     8641 acgaggccct ttcgtcttca agaatgatct agccctttcc ttaattaacc cgggcctctc
     8701 actctctgat attcatttct ttgcaagtta taaatactga ataataagat gacatgaact
     8761 actactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
     8821 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
     8881 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
     8941 agacccagta caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag
     9001 tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct
     9061 taaaggatct caggcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg
     9121 cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga
     9181 ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag
     9241 gcgagcagcc aaggaaagga cgatgatttc cccgacaaca ccacggaatt gtcagtgccc
     9301 aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca
     9361 atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa
     9421 ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc
     9481 cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc
     9541 atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa
     9601 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
     9661 ttaggatcta tcgcgataag cttgatatcg aattgggagg gactaaacaa caacaattgc
     9721 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     9781 ctctacaaat gtggtatggc tgattatgat ctagagtcgc ggccgcttta cttgtacatc
     9841 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     9901 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     9961 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
    10021 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
    10081 cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg gctggcgcga
    10141 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
    10201 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
    10261 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
    10321 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
    10381 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
    10441 ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
    10501 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
    10561 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
    10621 cttgttcaat catggtggac cggtaagctt atcatcgtgt ttttcaaagg aaaaccacgt
    10681 ccccgtggtt cggggggcct agacgttttt ttaacctcga ctaaacacat gtaaagcatg
    10741 tgcaccgagg ccccagatca gatcccatac aatggggtac cttctgggca tccttcagcc
    10801 ccttgttgaa tacgcttgag gagagccatt tgactctttc cacaactatc caactcacaa
    10861 cgtggcactg gggttgtgcc gcctttgcag gtgtatctta tacacgtggc ttttggccgc
    10921 agaggcacct gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg
    10981 tttgtcttca agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca
    11041 ttcctttggc gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt
    11101 tccgggccct cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg
    11161 cacaccggcc ttattccaag cggcttcggc cagtaacgtt agg
//
