LOCUS       sequence-425055-        7224 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Red fluorescent, negative response-polarity voltage indicator under 
            the control of synapsin promoter; soma-targeted; for better 
            photostability.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Han Y, Yang J, Li Y, Chen Y, Ren H, Ding R, Qian W, Ren K, Xie B, 
            Deng M, Xiao Y, Chu J, Zou P
  TITLE     Bright and sensitive red voltage indicators for imaging action 
            potentials in brain slices and pancreatic islets.
  JOURNAL   Sci Adv. 2023 Nov 24;9(47):eadi4208. doi: 10.1126/sciadv.adi4208. 
            Epub 2023 Nov 22.
  PUBMED    37992174
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-11-24"; volume: "9(47):eadi4208. doi"; pages: " 
            10.1126/sciadv.adi4208. Epub 2023 Nov 22"
COMMENT     Sequence Label: pAAV-hSyn-Cepheid1s-ST-WPRE
FEATURES             Location/Qualifiers
     source          1..7224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   16..145
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        356..803
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      816..825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3372..3960
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3425..3445)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3843..3854)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3980..4456
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4179..4198)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4502..4642
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4502..4631
                     /label=AAV2 ITR
     rep_origin      4717..5172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4804..4823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5014..5035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5189..5208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5308..5330
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5368..5386)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5454..5558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5559..5627,5628..6419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5777..5796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6590..7178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7079..7098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttttgctca catgtcctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg
       61 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
      121 gccaactcca tcactagggg ttcctgcggc cgcacgcgtt gcaaagatgg ataaagtttt
      181 aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg
      241 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
      301 gaggggtcgg cagcaaatgg ttaattaatc tagactgcag agggccctgc gtatgagtgc
      361 aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg
      421 acccactgga caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg
      481 ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag
      541 tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga
      601 cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg
      661 ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc
      721 atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag
      781 tcgtgtcgtg cctgagagcg cagtcgagag gatccgccac catggctgac gtggaaaccg
      841 agaccggcat gattgcacag tggattgtct ttgctattat ggctgctgct gctattgctt
      901 ttggagtggc tgtgcacttt cggccttcag agctgaagag cgcatactat atcaacattg
      961 ccatctgcac tatcgccgct accgcttact atgcaatggc cgtgaactac caggacctga
     1021 caatgctgat caaggaagag atgcccatga aggtggtcat gacaggtacc gtcaacggcc
     1081 actacttcaa gtgcacaggt gaaggagaag gcagaccgta cgagggagtg caaaccatga
     1141 ggatcaaagt catcgaggga ggacccctgc catttgcctt tgacattctt gccacgtcgt
     1201 tcatgtatgg cagccgtacc tttatcaagt acccggccga catccctgat ttctttaaac
     1261 agtcctttcc tgagggtttt acttgggaaa gagttacgag atacgaagat ggtggagtcg
     1321 tcaccgtcac gcaggacacc agccttcagg atggcgtgct catctacaac gtcaaggtca
     1381 gaggggagaa ctttccctcc aatggtcccg tgatgcagaa gaagaccaag ggttgggagc
     1441 ctaatacaga gatgatgtat ccagcagatg gtggtctgag aggatacact gacatcgcac
     1501 tgaaagttga tggtggtggc catctgcact gcagcttcgt gacagaatac aagtcaaaaa
     1561 agaccgtcgg gaacatcaag atgcccggtg tccatgccgt tgatcaccgc ctggaaagga
     1621 tcgaggagag tgacaatgaa acctacgtag tgcaaagaga agtggcagtt gccaaataca
     1681 gcaaccttgg tggtggcatg gacgagctgt acaagaatgg tgaaaggcag gtggtctacg
     1741 caagatatat ttgttgggtg ctgaccacac cactgctcct gctcgatctc atcgtcatga
     1801 ccaagatggg cggagtgatg atttcttggg tcatcggcgc agacattttc atgatcgtgt
     1861 ttggtattct gggcgccttc gaggatgaac acaagttcaa atgggtgtac tttatcgctg
     1921 gatgtgtgat gcaggcagtc ctgacatacg ggatgtataa cgccacttgg aaagacgatc
     1981 tgaagaaaag ccccgagtac catagctcct atgtcagtct gctcgtcttc ctgtcaatcc
     2041 tctgggtgtt ttatcctgtc gtgtgggctt tcgggtctgg tagtggcgtg ctgtccgtcg
     2101 acaatgaggc cattctcatg ggaatcctgg atgtgctcgc taagccactg tttggaatgg
     2161 ggtgcctcat tgcccatgag actatcttca agaccggtgc cgccgaccgc ccggtagtag
     2221 cagtgagcaa ggcggccgcc gctagcaaat caaggatcac ctctgagggc gagtatatcc
     2281 ctctggatca gatcgacatt aacgtcggtg gaaaatcccg cataacctct gagggcgagt
     2341 acatccctct ggatcaaatc gatataaacg tggggggaaa aagtcgaatc acttctgagg
     2401 gcgagtatat acccctcgat cagattgaca tcaatgtggg gggcaacatg gccatcatca
     2461 aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga
     2521 tcgagggcga gggcgagggc cgcccctacg agggctttca gaccgctaag ctgaaggtga
     2581 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcatttc acctttggct
     2641 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttcaagctg tccttccccg
     2701 agggcttcaa gtgggagcgc gtgatgaact acgaggacgg cggcgtggtg accgtgaccc
     2761 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     2821 tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     2881 ggatgtaccc cgaggacggt gccctgaagg gcaagatcaa gatgaggctg aagctgaagg
     2941 acggcggcca ctacacctcc gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     3001 tgcccggcgc ctacatcgtc gacatcaagt tggacatcac ctcccacaac gaggactaca
     3061 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     3121 tgtacaagca gagccagcct atcctgaaca ccaaggagat ggcccctcag agcaagcctc
     3181 ctgaggagct ggagatgagc agcatgccta gccctgtggc ccctctgcct gccaggaccg
     3241 agggcgtgat cgacatgagg agcatgagca gcatcgacag cttcatcagc tgcgccaccg
     3301 acttccctga ggccaccagg ttcttctgct atgaaaatga agtgtaagaa ttcgatatca
     3361 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3421 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3481 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3541 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3601 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3661 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3721 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     3781 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3841 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3901 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3961 atcgataccg gctcgagacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     4021 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     4081 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     4141 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     4201 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     4261 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     4321 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     4381 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     4441 tcccttccct gtccttctcg agctgatttt gtaggtaacc acgtgcggac cgagcggccg
     4501 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4561 gccgggcgac caaaggtcgc cctacgcccg ggctttgccc gggcggcctc agtgagcgag
     4621 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4681 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4741 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     4801 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4861 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4921 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4981 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5041 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     5101 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5161 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5221 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5281 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5341 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5401 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5461 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5521 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5581 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5641 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5701 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5761 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5821 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5881 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5941 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6001 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6061 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6121 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6181 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6241 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6301 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6361 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6421 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6481 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6541 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6601 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6661 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6721 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6781 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6841 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6901 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6961 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7021 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7081 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7141 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7201 tacggttcct ggccttttgc tggc
//
