LOCUS       sequence-425105-        2025 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  used for TALEN HDR to generate endogenous fusion of HA-Dendra2 to 
            apoBb.1 in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2025)
  TITLE     The Photoconvertible LipoTimer Reporter Allows for Direct In Vivo 
            Measuring of Atherogenic Lipoprotein Kinetics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: apoBb.1-Dendra2
FEATURES             Location/Qualifiers
     source          1..2025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             538..564
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             577..1269
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 ctgcctggaa tgaatgaagc tacactggct gaaatttgca agaaaatcca attagttgtt
       61 gtagaaatgc tgaggaattt tggtaacaac ttagaggtat acttctctcc catcatggat
      121 aactttaata caattcaatt gacatttccc aatggaaagg tcatgacagt agctgagttc
      181 caacaaaatg tgcaaagtat cctgagaagc aacctagtta tgatagcaga tgcaatgaag
      241 caaatcgaaa gtcctgatgt agttcttaag aaacttggac aaactttaca agaggttgtt
      301 gaaaaaggac aagaatttgt ggacaaaatg aatttaagtt tattagaaga tattgccgcg
      361 gccattaaca cgttctacca ggagctgatg aagatcatag aagacattag tgaggcagtc
      421 atctttggtt tttcaatccc tgcaattaaa atatatgaaa tttctcaaaa catagggaaa
      481 gtgttgaatg caaataatgg aatacatcaa tttgagcttc ctctcccatt tttccagtac
      541 ccatacgatg ttccagatta cgctggtggc ggtggaatga acaccccggg aattaacctg
      601 atcaaggagg acatgcgcgt gaaggtgcac atggagggca acgtgaacgg ccacgccttc
      661 gtgatcgagg gcgagggcaa gggcaagccc tacgagggca cccagaccgc caacctgacc
      721 gtgaaggagg gcgcccccct gcccttcagc tacgacatcc tgaccaccgc cgtgcactac
      781 ggcaaccggg tgttcaccaa gtaccccgag gacatccccg actacttcaa gcagagcttc
      841 cccgagggct acagctggga gcgcaccatg accttcgagg acaagggcat ctgcaccatc
      901 cgcagcgaca tcagcctgga gggcgactgc ttcttccaga acgtgcgctt caagggcacc
      961 aacttccccc ccaacggccc cgtgatgcag aagaagaccc tgaagtggga gcccagcacc
     1021 gagaagctgc acgtgcgcga cggcctgctg gtgggcaaca tcaacatggc cctgctgctg
     1081 gagggcggcg gccactacct gtgcgacttc aagaccacct acaaggccaa gaaggtggtg
     1141 cagctgcccg acgcccactt cgtggaccac cgcatcgaga tcctgggcaa cgacagcgac
     1201 tacaacaagg tgaagctgta cgagcacgcc gtggcccgct acagccccct gcccagccag
     1261 gtgtggtaaa atatggtcat tctgaacaga aagtaaatta agtaaacact accatctttt
     1321 gaaatgaaca ttcttggaca tcgaagccaa tggacacttc actgaagtat agtacaaaca
     1381 tgtatataaa gcaattactg actcaaaaca tgtctcattg ttatgctgct ctattattct
     1441 atttgtataa atgaggattg aaatactaag ctagttatca aaaatgtcaa ctgcaaccta
     1501 ttgtcatcct tgttctgtat ccaacattaa tggcatgatg gatggggatc aataaagaac
     1561 ttttattatg caacacgacc caatcttgtc atttctatat tctagaagtg ttttaaacta
     1621 gtttggacca ttcaataata taatataatt gaatatacag gtgtaataac atgcaacaaa
     1681 atgaaaaata agcagtggta catgggtaca ttttagtttt ttatgcaaac tactggtgac
     1741 ttaaaaaaaa ttacaaataa aatattttat tctatttttt tcattaggaa gaaaaaagtt
     1801 tatcagaact tttgtagagg taaaaaaaac tactaacatt ttactcaatc ccatacctga
     1861 aaaatctagt aaatcaattg ctttgattag tggttattga accttatttt aaccagcttc
     1921 aggttaacac atgttaacta ctcctttttg acatataaac tgaaaatcat ttaatcttca
     1981 ttaaaaatat tttggatttg cttacaaact gatgtctgcc ttacc
//
