LOCUS       sequence-425138-        9209 bp    DNA     circular SYN 15-APR-2025
DEFINITION  A pUra4 backbone integrating plasmid expressing mTurquoise2 under 
            the inducible promoter 41nmt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Pan ZQ, Yang YS, Du LL
  TITLE     Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in 
            the Fission Yeast Schizosaccharomyces pombe.
  JOURNAL   Methods Mol Biol. 2025;2862:93-102. doi: 
            10.1007/978-1-0716-4168-2_7.
  PUBMED    39527195
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2025"; volume: "2862:93-102. doi"; pages: " 
            10.1007/978-1-0716-4168-2_7"
COMMENT     Sequence Label: pDB4887
FEATURES             Location/Qualifiers
     source          1..9209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2198..2217)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2317..2339
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2377..2395)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2463..2567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2568..2636,2637..3428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2786..2805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3599..4187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4088..4107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4341..4358
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4475..4496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4511..4516,4517..4534,4535..4541)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4549..4565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4554..4576
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4573..4589
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4573..4589
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4608..4628
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4610..4628
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     gene            complement(4690..5809)
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     terminator      complement(4690..4887)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(4893..5465)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(5466..5809)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      complement(5878..6065)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     5986..6010
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(8000..8019)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8001..8019)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8029..8046)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8029..8045)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8038..8060)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8186..8641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8273..8292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8483..8504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(9133..9209,1..718))
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
ORIGIN
        1 gaccagctcc ataaactcca cgaccaacaa tgatgatatc gctaccgcag tttacaatca
       61 cttcttcagg agtacgatat tgctgtccca gcccgtctcc tttaacatcc aagccgatac
      121 caggggacat agttatgtag tcgctttgaa ggttaggaaa tcgacgacca gctataaagc
      181 caaagcaaaa atcggtatgc ttctcaaacc attctaaggt tttctctgtg taggaaccag
      241 tagccaaaga gcctttggaa gacatttcag ccaaaagcaa gagaccacgt cccaaaggta
      301 aaccaacttc tttgaggcct tgtataatac cctcgcctgg cactgtatgg caatttgtga
      361 tatgagccca agaagcaatt ttgtacacac cagatgcata ttgtagcttg acggtatttc
      421 caatgtctgc gaatttgcga tcctcaaaga taagaaaacg atgcttttta cctaaggcca
      481 ccagtttttc taccatatcc tggtcgaaat cctcgacaac gtcaatatgt gtcttgataa
      541 cacagacata gggtccaatt ttatctacca attctaagat ttcggatttc ttcgtcaaat
      601 cgaccgcgac tgacaagttg ctttgctttt cttccatcaa agccaacaat tccttggcaa
      661 tgggattttt catcccctca gctctagctg aatagctttg aaatactcta gcatccataa
      721 ctttgctttt aaacctttaa tttcgatcca agcaaaaaag aggttcttgg taggacaata
      781 cggtaagaaa acacgacatg tgcagagatg ccgacgaagc atagttaaac tgggatggta
      841 aaatcaatta agaatttata aagacaaaat tgtataagtc tctaaaacat cttaattata
      901 cctcacagaa ctatctaaaa tatattcaca aagtgcaaac attatcatga aaaagaacca
      961 ttttaattta aagcaagggc attaaggctt atttacagaa tttcttactt ttgtaaagat
     1021 tataaggctg attatctttt tcaccatgcc aaaaattaca caagatagaa tggatgtttg
     1081 aaattaaacg tgagtataca aacaaataca ctaggtaaat cgaaacattt ttttctccat
     1141 taagtaacaa attcctattt agagaaagaa tgctgagtag attaaataat ctatacaaac
     1201 ttttttaaca caaatgcata catatagcca gtgggatttg tagctaagct tcaggagttt
     1261 tatccattta atgtatggaa tcaaaattta aagcttctgt caaagtttaa caatatttct
     1321 tttggtttaa atcaaatctt ccatgcgatt aagaagatag atgctgaaca aaagaagcac
     1381 atggataacc acaaaagcag tttgctcatg ggtacaacca tgaatttttt tttcgacaat
     1441 gattcaaaga ccagtatatc caatacgcat cctagaatct agtcaaagaa gaacctaaag
     1501 tagagatgca aatgcgctaa aaagagtgga tataaattca atatcattta taaaacaact
     1561 tcttccatta aaaattcctt gggcaaaaca aaagttccaa tcataaaaag ttaataagtt
     1621 ctgagttgtg tcaaatctga catggcattc ctcaataatg acactcactc attatgtaag
     1681 cagcggccgc tgctctgtac acgtattctt tccctcttat cattcctgtt tttctttttt
     1741 aataaaccaa catgcctgta agtagtttta tctttagaaa tctgtgcatc atggatccta
     1801 actatgtctt ttagacggtt caaactccct ctcagcgtcg tgcaaacaca cagtttcaga
     1861 agaacattac tcgtcgtgta aaaacaaatt caaaagaacg ttatgttgcc aaacatcctc
     1921 ctaccaagat tcctcgtaac attgccagta agtaagaatt gatcctattg ttagcaactt
     1981 tggcttgtgt ttcatactga caatgcatct tagtgttttt tattcttctc atgtcaggag
     2041 gaattatttt gggaatactt agatttcttt tgacgttttt ttcttaagaa catgtgattg
     2101 gagcaatttt aaaacctatt tgcaccgata ttgtgtatta tactccgaga aaaagtatac
     2161 tagttttgac taatacccgg ttatggtgca ctctcagtac aatctgctct gatgccgcat
     2221 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2281 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2341 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     2401 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     2461 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2521 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2581 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2641 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2701 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2761 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2821 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2881 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2941 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3001 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3061 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3121 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3181 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3241 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3301 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3361 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3421 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3481 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3541 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3601 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3661 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3721 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3781 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3841 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3901 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3961 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4021 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4081 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4141 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4201 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4261 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4321 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4381 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4441 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     4501 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     4561 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     4621 actaaaggga acaaaagctg gcacgtgatt acctgcaggt ggatggcggc gttagtatcg
     4681 aatcgacagc agtatagcga ccagcattca catacgattg acgcatgata ttactttctg
     4741 cgcacttaac ttcgcatctg ggcagatgat gtcgaggcga aaaaaaatat aaatcacgct
     4801 aacatttgat taaaatagaa caactacaat ataaaaaaac tatacaaatg acaagttctt
     4861 gaaaacaaga atctttttat tgtcagtact gattaggggc agggcatgct catgtagagc
     4921 gcctgctcgc cgtccgaggc ggtgccgtcg tacagggcgg tgtccaggcc gcagagggtg
     4981 aaccccatcc gccggtacgc gtggatcgcc ggtgcgttga cgttggtgac ctccagccag
     5041 aggtgcccgg caccccgctc gcgggcgaac tccgtcgcga gccccatcaa cgcgcgcccg
     5101 accccgtgcc cccggtgctc cggggcgacc tcgatgtcct cgacggtcag ccggcggttc
     5161 cagccggagt acgagacgac cacgaagccc gccaggtcgc cgtcgtcccc gtacgcgacg
     5221 aacgtccgag agtccgggtc gccgtcctcc ccgtcgtccg attcgtcgtc cgattcgtcg
     5281 tcggggaaca ccttggtcag gggcgggtcc accggcacct cccgcagggt gaagccgtcc
     5341 ccggtggcgg tgacgcggaa gacggtgtcg gtggtgaagg acccatccag tgcctcgatg
     5401 gcctcggcgt cccccgggac actggtgcgg taccggtaag ccgtgtcgtc aagagtggta
     5461 cccatggttg tttatgttcg gatgtgatgt gagaactgta tcctagcaag atttaaaaag
     5521 gaagtatatg aaagaagaac ctcagtggca aatcctaacc ttttatattt ctctacaggg
     5581 gcgcggcgtg gggacaattc aacgcgtctg tgaggggagc gtttccctgc tcgcaggtct
     5641 gcagcgagga gccgtaattt ttgcttcgcg ccgtgcggcc atcaaaatgt atggatgcaa
     5701 atgattatac atggggatgt atgggctaaa tgtacgggcg acagtcacat catgcccctg
     5761 agctgcgcac gtcaagactg tcaaggaggg tattctgggc ctccatgtcg ctggccgggt
     5821 gacccggcgg ggacgaggca agctaaacat attaccctgt tatccctagc ggatctgccg
     5881 gtagaggtgt ggtcaataag agcgacctca tactatacct gagaaagcaa cctgacctac
     5941 aggaaagagt tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat
     6001 ttgtatacac ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa
     6061 ttcgcttatt tagaagtggc gcgttaagat ctcttgtaca attcatccat acccaaggta
     6121 ataccggcag cggtaacaaa ttccaaaagg accatgtgat cacgtttttc attaggatct
     6181 ttagacaatt tggattgagt agaaagataa tggttatcag gcaacaaaac aggaccgtca
     6241 ccaataggag tgttttgttg gtaatgatca gcaagttgta ctccgccatc ttcaatgtta
     6301 tgacgaatct tgaagttagc cttgatacca ttcttttgtt tatcggcagt aatgtaaaca
     6361 ttatcagaaa agtaattgta ttctaattta tgaccaagaa tattaccatc ctctttaaaa
     6421 tcaattcctt tgagctcaat acggttaact aaagtatcac cctcaaattt aacttcagca
     6481 cgagtcttat agttaccatc gtctttgaag aaaatagtac gctcttgaac ataaccttca
     6541 ggcatagcac tcttaaagaa atcgtgttgc ttcatatggt caggataacg agcaaagcat
     6601 tgaacacccc aagacagggt agtgacaaga gtaggccaag ggacaggaag ttttccagta
     6661 gtgcaaatga atttaagggt aagcttacca taagtagcat caccttctcc ttcaccagag
     6721 actgaaaact tgtgtccatt aacatctcca tcaagttcaa ccaagatagg aacgacacca
     6781 gtaaagagtt cttcaccctt agaaaccatg ctagctttaa caaagcgact ataagtcaga
     6841 aagtgagaat gagattgaaa taattaattc aacttattca attgatgata tgccaggatt
     6901 cctcttcctt tatcttcaat gtttcgatta acaggtccat tatccggttt ttagtttcgc
     6961 tgcacattgc cgaatgacaa tcggattcct ttaagctttg cttttgccct gttttctctc
     7021 cccagtaatc gttgggtgac tgaaccatcc agtagccgag agttacgttt ttcctgattt
     7081 aataaatatc catatctatt tgtctcgtgg tgttttacag caactaaccg aaattactat
     7141 tttgcatcat ccaaccataa agagacaaca ataattctag aaaacatata acaagtgttt
     7201 ttttctattt acgtctcata cgcaaagtcc actttaccat gttttggaaa cttgtaccca
     7261 accattcatt tttcgatttt gtaatgaggc tcttttcttc taacaatcat tgaggtcatt
     7321 atcatgtgtc ctcacctatt gtttaaattg aggcttgcgc tattcccaaa attgtgggta
     7381 ttattttcat atatcagtat ctgcaatttc ggttcaactt tcttatcaag aaagaggatt
     7441 aatttcttaa caaaaacctt ctttgtttta taacgctgat ttttgtatta tttggcgacc
     7501 aaggttataa gtgatccaat ccaaatgtca taacttcagt tgatccctgc ggaaaatata
     7561 aatcagatta tggttatata aaaaaaaaga gaaattattt gagttcattg aaacttcaac
     7621 gttatttgac taaaacttgt taatttacat gtctgcaaga ttaatccagc atattaaata
     7681 aattagttga cttatgtcaa acaaataata cattcttgat attattagaa aaaaaaaaga
     7741 aaattgaata acttgtgaga aatgatattg ttattatttt agattatgtc ctttgaaata
     7801 caataacatg ctaaggcact tactacttaa gcctttatta ctttaatgtg ctaacaatct
     7861 tctgtatcct tagacgttaa attttccctt attaaaaaat aaaaaaataa aaaaaaaata
     7921 taggaaatga aacaaccgct gatgacttat tctgtctttt atggcgataa ttttctgatc
     7981 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     8041 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     8101 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     8161 gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8221 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8281 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8341 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8401 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8461 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8521 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8581 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     8641 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc cctagcaagc
     8701 ttgtgatatt gacgaaactt tttgacatct aatttattct gttccaacac caatgtttat
     8761 aaccaagttt tatcttgttt gtctacatgg tattttacat tcatctacat acatctttca
     8821 ttggctttgt acatagttat cattacaagt ctaaaaaaat tcactctttt cttattcaat
     8881 gtcaatccaa gagaaaagat tgtggtaatg ttgtaggagc atgtttaata aattactata
     8941 gcaaattact ttttattccc aaggtgttta tctataatag ttaatatttt agtcgctaca
     9001 taaaatttta ccaaagagta cttgtatact aattctaaat gccttctgac ataaaacgcc
     9061 taggaaaaca aacgcaaaca aggcatcgac tttttcaata accaaccaaa aaaattttac
     9121 attagtcttt ttttaatgct gagaaagtct ttgctgatat gccttccaac cagcttctct
     9181 atatctcttg gcttcgacaa caggattac
//
