LOCUS       sequence-425146-        9433 bp    DNA     circular SYN 15-APR-2025
DEFINITION  A pAde6 backbone integrating plasmid expressing mECitrine under the 
            inducible promoter 41nmt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Pan ZQ, Yang YS, Du LL
  TITLE     Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in 
            the Fission Yeast Schizosaccharomyces pombe.
  JOURNAL   Methods Mol Biol. 2025;2862:93-102. doi: 
            10.1007/978-1-0716-4168-2_7.
  PUBMED    39527195
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2025"; volume: "2862:93-102. doi"; pages: " 
            10.1007/978-1-0716-4168-2_7"
COMMENT     Sequence Label: pDB4878
FEATURES             Location/Qualifiers
     source          1..9433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2707..3162)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2844..2865)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3056..3075
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3288..3310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3302..3319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3303..3319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3329..3348
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3329..3347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4569..4597)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5192..5212
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      5280..5467
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5335..5359)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            5536..7111
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        5536..5879
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5880..6908
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMRLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVGASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      6914..7111
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7173..7193)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7173..7191)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7212..7228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7212..7228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7225..7247)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7236..7252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7260..7266,7267..7284,7285..7290))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7305..7326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7443..7460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7614..8202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7694..7713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8373..9164,9165..9233))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8996..9015
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9234..9338)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9406..9424
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttagc ggccgctaaa cgttgcttta tatatggata
      601 acgacatata catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct
      661 gctttaaagt tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc
      721 aaaactcaaa taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat
      781 tcgttttttc aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca
      841 tcgaatatga gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg
      901 atggtagagg cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct
      961 cctgccaaac aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat
     1021 gcaattgttg aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac
     1081 actgatgcct tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg
     1141 cgatgcattc aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt
     1201 cctgaatttt gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt
     1261 tatccttttg tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt
     1321 catcaaccat ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt
     1381 gaaaagttcg ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga
     1441 aaagtttatg cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta
     1501 tatgcccctg ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat
     1561 gcggtgcgca cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat
     1621 ggtgagacca ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc
     1681 attgaagctt gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct
     1741 ttcagcgaaa tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt
     1801 ttaggtactg atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc
     1861 ggtgcaactt tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac
     1921 gttaccatca tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac
     1981 gtcaaagatc ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta
     2041 agcaagatga aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact
     2101 attgtttcag ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca
     2161 agagggttgc gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt
     2221 gctgcaatga cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga
     2281 gttgactctc ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct
     2341 atcaataata gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc
     2401 tcccttttgg ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag
     2461 gctgataaat tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata
     2521 actgttaaat gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga
     2581 ttgtaaaatg tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt
     2641 gctgcgacgc agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2701 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2761 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2821 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2881 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2941 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3001 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3061 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3121 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     3181 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     3241 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     3301 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     3361 cgggcccgat cagaaaatta tcgccataaa agacagaata agtcatcagc ggttgtttca
     3421 tttcctatat ttttttttta tttttttatt ttttaataag ggaaaattta acgtctaagg
     3481 atacagaaga ttgttagcac attaaagtaa taaaggctta agtagtaagt gccttagcat
     3541 gttattgtat ttcaaaggac ataatctaaa ataataacaa tatcatttct cacaagttat
     3601 tcaattttct tttttttttc taataatatc aagaatgtat tatttgtttg acataagtca
     3661 actaatttat ttaatatgct ggattaatct tgcagacatg taaattaaca agttttagtc
     3721 aaataacgtt gaagtttcaa tgaactcaaa taatttctct ttttttttat ataaccataa
     3781 tctgatttat attttccgca gggatcaact gaagttatga catttggatt ggatcactta
     3841 taaccttggt cgccaaataa tacaaaaatc agcgttataa aacaaagaag gtttttgtta
     3901 agaaattaat cctctttctt gataagaaag ttgaaccgaa attgcagata ctgatatatg
     3961 aaaataatac ccacaatttt gggaatagcg caagcctcaa tttaaacaat aggtgaggac
     4021 acatgataat gacctcaatg attgttagaa gaaaagagcc tcattacaaa atcgaaaaat
     4081 gaatggttgg gtacaagttt ccaaaacatg gtaaagtgga ctttgcgtat gagacgtaaa
     4141 tagaaaaaaa cacttgttat atgttttcta gaattattgt tgtctcttta tggttggatg
     4201 atgcaaaata gtaatttcgg ttagttgctg taaaacacca cgagacaaat agatatggat
     4261 atttattaaa tcaggaaaaa cgtaactctc ggctactgga tggttcagtc acccaacgat
     4321 tactggggag agaaaacagg gcaaaagcaa agcttaaagg aatccgattg tcattcggca
     4381 atgtgcagcg aaactaaaaa ccggataatg gacctgttaa tcgaaacatt gaagataaag
     4441 gaagaggaat cctggcatat catcaattga ataagttgaa ttaattattt caatctcatt
     4501 ctcactttct gacttatagt cgctttgtta aagctagcat gagtaaagga gaagaacttt
     4561 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     4621 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
     4681 gcactactgg aaaactacct gttccatggc caacacttgt cactactttg ggttatggtc
     4741 taatgtgctt tgctagatac ccagatcata tgaaacggca tgactttttc aagagtgcca
     4801 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     4861 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
     4921 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
     4981 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
     5041 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     5101 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcctatcaa tctaagcttt
     5161 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     5221 ggattacaca tggcatggat gaactataca aaagatctta acgcgccact tctaaataag
     5281 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
     5341 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
     5401 tcctgtaggt caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct
     5461 ctaccggcag atccgctagg gataacaggg taatatgttt agcttgcctc gtccccgccg
     5521 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
     5581 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa
     5641 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg
     5701 ctgcggacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc
     5761 gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata
     5821 cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca
     5881 tgggtaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     5941 acagcgtctc cgacctgatg cggctctcgg agggcgaaga atctcgtgct ttcagcttcg
     6001 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     6061 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     6121 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     6181 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     6241 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     6301 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     6361 atcactggca aactgtgatg gacgacaccg tcggtgcgtc cgtcgcgcag gctctcgatg
     6421 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     6481 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     6541 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     6601 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     6661 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     6721 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     6781 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     6841 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     6901 aggaataatc agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata
     6961 gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt
     7021 tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt
     7081 caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc
     7141 acctgcaggt aatcacgtgc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     7201 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7261 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7321 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7381 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7441 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7501 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7561 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7621 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7681 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7741 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7801 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7861 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7921 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7981 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8041 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8101 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8161 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8221 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8281 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8341 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8401 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8461 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8521 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8581 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8641 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8701 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8761 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8821 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8881 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8941 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9001 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9061 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9121 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9181 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9241 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9301 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9361 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     9421 aggccctttc gtc
//
