LOCUS       sequence-42552-e        2669 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2669)
  TITLE     Jing Wang lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUAST_mLExA-VP16-NFATc1 (C-del)
FEATURES             Location/Qualifiers
     source          1..2669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(12..263,264..617)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKGLEKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     555..576
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             663..896
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 gcggccgcgg aatgaaagcg ttaacggcca ggcaacaaga ggtgtttgat ctcatccgtg
       61 atcacatcag ccagacaggt atgccgccga cgcgtgcgga aatcgcgcag cgtttggggt
      121 tccgttcccc aaacgcggct gaagaacatc tgaaggcgct ggcacgcaaa ggcgttattg
      181 aaattgtttc cggcgcatca cgcgggattc gtctgttgca ggaagaggaa gaagggttgc
      241 cgctggtagg tcgtgtggct gccggtgaac cacttctggc gcaacagcat attgaaggtc
      301 attatcaggt cgatccttcc ttattcaagc cgaatgctga tttcctgctg cgcgtcagcg
      361 ggatgtcgat gaaagatatc ggcattatgg atggtgactt gctggcagtg cataaaactc
      421 aggatgtacg taacggtcag gtcgttgtcg cacgtattga tgacgaggtt accgttaagg
      481 gcctggaaaa acagggcaat aaagtcgaac tgttgccaga aaatagcgag tttaaaccaa
      541 ttgtcgtaga tcttcgtcag cagagcttca ccattgaagg gctggcggtt ggggttattc
      601 gcaacggcga ctggctggaa ttcccgggga tccgtcgacc tgcaggaatt cccggggatc
      661 tggccccccc gaccgatgtc agcctggggg acgagctcca cttagacggc gaggacgtgg
      721 cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt
      781 ccccgggtcc gggatttacc ccccacgact ccgcccccta cggcgctctg gatatggccg
      841 acttcgagtt tgagcagatg tttaccgatg cccttggaat tgacgagtac ggtgggtcta
      901 gaatgccaag caccagcttt ccagtccctt ccaagtttcc acttggccct gcggctgcgg
      961 tcttcgggag aggagaaact ttggggcccg cgccgcgcgc cggcggcacc atgaagtcag
     1021 cggaggaaga acactatggc tatgcatcct ccaacgtcag ccccgccctg ccgctcccca
     1081 cggcgcactc caccctgccg gccccgtgcc acaaccttca gacctccaca ccgggcatca
     1141 tcccgccggc ggatcacccc tcggggtacg gagcagcttt ggacggtggg cccgcgggct
     1201 acttcctctc ctccggccac accaggcctg atggggcccc tgccctggag agtcctcgca
     1261 tcgagataac ctcgtgcttg ggcctgtacc acaacaataa ccagtttttc cacgatgtgg
     1321 aggtggaaga cgtcctccct agctccaaac ggtccccctc cacggccacg ctgagtctgc
     1381 ccagcctgga ggcctacaga gacccctcgt gcctgagccc ggccagcagc ctgtcctccc
     1441 ggagctgcaa ctcagaggcc tcctcctacg agtccaacta ctcgtacccg tacgcgtccc
     1501 cccagacgtc gccatggcag tctccctgcg tgtctcccaa gaccacggac cccgaggagg
     1561 gctttccccg cgggctgggg gcctgcacac tgctgggttc cccgcggcac tccccctcca
     1621 cctcgccccg cgccagcgtc actgaggaga gctggctggg tgcccgctcc tccagacccg
     1681 cgtccccttg caacaagagg aagtacagcc tcaacggccg gcagccgccc tactcacccc
     1741 accactcgcc cacgccgtcc ccgcacggct ccccgcgggt cagcgtgacc gacgactcgt
     1801 ggttgggcaa caccacccag tacaccagct cggccatcgt ggccgccatc aacgcgctga
     1861 ccaccgacag cagcctggac ctgggagatg gcgtccctgt caagtcccgc aagaccaccc
     1921 tggagcagcc gccctcagtg gcgctcaagg tggagcccgt cggggaggac ctgggcagcc
     1981 ccccgccccc ggccgacttc gcgcccgaag actactcctc tttccagcac atcaggaagg
     2041 gcggcttctg cgaccagtac ctggcggtgc cgcagcaccc ctaccagtgg gcgaagccca
     2101 agcccctgtc ccctacgtcc tacatgagcc cgaccctgcc cgccctggac tggcagctgc
     2161 cgtcccactc aggcccgtat gagcttcgga ttgaggtgca gcccaagtcc caccaccgag
     2221 cccactacga gacggagggc agccgggggg ccgtgaaggc gtcggccgga ggacacccca
     2281 tcgtgcagct gcatggctac ttggagaatg agccgctgat gctgcagctt ttcattggga
     2341 cggcggacga ccgcctgctg cgcccgcacg ccttctacca ggtgcaccgc atcacaggga
     2401 agaccgtgtc caccaccagc cacgaggcca tcctctccaa caccaaagtc ctggagatcc
     2461 cactcctgcc ggagaacagc atgcgagccg tcattgactg tgccggaatc ctgaaactca
     2521 gaaactccga cattgaactt cggaaaggag agacggacat cgggaggaag aacacacggg
     2581 tacggctggt gttccgcgtt cacgtcccgc aacccagcgg acgcacgctg tccctgcagg
     2641 tggcctccaa ccccatcgaa tgctcctga
//
