LOCUS       sequence-42575-e         869 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: pmTagBFP2-C1
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..758
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    759..824
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcggt ggccaccatg gtgtctaagg
       61 gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
      121 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
      181 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
      241 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
      301 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
      361 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
      421 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
      481 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
      541 ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca
      601 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
      661 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
      721 actgcgacct ccctagcaaa ctggggcaca agcttaattc cggactcaga tctcgagctc
      781 aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac
      841 tgatcataat cagccatacc acatttgta
//
