LOCUS       sequence-426124-        6141 bp    DNA     circular SYN 15-APR-2025
DEFINITION  competition strain 5 integrase plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Glass DS, Bren A, Vaisbourd E, Mayo A, Alon U
  TITLE     A synthetic differentiation circuit in Escherichia coli for 
            suppressing mutant takeover.
  JOURNAL   Cell. 2024 Jan 31:S0092-8674(24)00061-8. doi: 
            10.1016/j.cell.2024.01.024.
  PUBMED    38320549
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Jan 31:S0092-8674(24)00061-8. doi: 10.1016/j.cell.2024.01.024."
COMMENT     Sequence Label: pDSG599
FEATURES             Location/Qualifiers
     source          1..6141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..72)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             2460..2489
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      2537..2623
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    2637..2657
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2693..2724
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     terminator      complement(2827..2921)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2933..3724)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(4082..5032)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFGIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5080..5302
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5794..5888)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(6044..6073)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    6120..6141
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
       61 tcgttttatt tgatgcctgg ggagtgtgag tgggaggtct gtatgtacgt cgagtacctt
      121 tggtcgaaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tccttaacgc
      181 tgtgtacccg gacaaacacc attaatcagc aggaaggtaa attcttcaat atcctgttca
      241 acggtcagct gttcggtcag taggcgatac caacaaaaac caaaaatcat atccagcagc
      301 agttcacgat tggtatcttt cggcagttca ccattgctaa tggcattttc aaccagtttt
      361 ttcggcatct cacgacgacg ttccataaac tgatctttca gctgggtcag ggttgcaggg
      421 tccagctgtg cttctgcaat aacacaacga aatgcttcac cacaaatggt ttcacgccaa
      481 actttccaca gattacgcag cagaaaatcc agatcggctt taaagctacc cagatccgga
      541 aatttacgca cctgttcgct ttcattttca tacacttcgg caatcagtgc tgctttattg
      601 gtccaccaac gataaatggt cggtttgctt gcaccggcac gacgtgcaac gctttcaatg
      661 ctcagaccgc tataaccaca ttctttcagg atttcaatgg tgctggtcag aattgcttta
      721 tgggtatgcg gactacgcag gctaccaatg ctgctacggc tcggggtacg tgccattaat
      781 tattcctact ttcagaggaa agttaaacaa aattatttgt agagggaaac cgttgtggtc
      841 tccctgaata tattatacga gccttatgca tgcccgtaaa gttatccagc aaccactcat
      901 agacctaggg cagcagatag ggacgacgtg gtgttagctg tgccgtcgga attgctatag
      961 cccttaacgc atgagaaagc ccccggaaga tcaccttccg ggggcttttt tattgcgctc
     1021 tgattcgtta ccaattgaca tgatacgaaa cgtaccgtat cgttaaggtt acttagccat
     1081 cccgaaatac ggatatttaa gtaatgaagg cagctattta tatacgtgtt tctactcaag
     1141 agcaagtaga aaattattca atacaagctc aaactgaaaa actaacagga ttgtgccgct
     1201 cgaaggactg ggacgtatac gatattttca ttgacggcgg atactccggc tcaaatatga
     1261 atcgtcccgc attaaatgaa atgctaagta aactacacga aattgatgct gtagtcgtat
     1321 atcgattaga cagactatcc cgctcacaaa gagacacaat aacgcttatt gaagaatact
     1381 tcttaaaaaa caatgtagag tttgttagtt tgtctgaaac gcttgatacc agttcccctt
     1441 tcggtcgtgc aatgattggt atattatcag tattcgcaca gctagagcgc gaaacaatcc
     1501 gagatcgtat ggtgatgggg aaaattaagc gtattgaagc aggtcttccg ttaacaactg
     1561 cgaaaggtag aacgttcggc tatgatgtta tagatacaaa attatacatt aatgaagaag
     1621 aagcaaaaca gttacaactg atttatgata ttttcgaaga agaacaaagt ataacttttt
     1681 tacagaaaag actaaaaaaa ttaggcttta aagttagaac atataatcgc tataacaact
     1741 ggctaactaa tgatttgtat tgtggttatg tttcatataa agataaagtt catgtaaaag
     1801 gtattcatga acctatcatc agtgaagagc aattctatag agttcaagaa atatttactc
     1861 gtatgggtaa aaatccgaac atgaatagag attcagcatc gttgctaaat aatttagtag
     1921 tttgtagtaa atgcgggtta ggctttgttc atcgtagaaa agatacaatg tcgcgtggta
     1981 aaaaatatca ttatagatat tatagttgca agacttataa acatactcat gaactcgaaa
     2041 aatgcgggaa taaaatttgg agagctgaca aacttgaaga attaattatt aatcgtgtga
     2101 ataattatag tttcgcttcc agaaatgtag ataaagaaga tgaattagat agcttaaatg
     2161 aaaagcttaa aatagaacat gcaaagaaaa aacgattatt tgatttatat ataaatggct
     2221 cgtatgaagt ttcagaactt gattctatga tgaatgatat tgatgctcaa attaattatt
     2281 atgaatcaca aatagaagct aacgaagaat tgaagaaaaa caaaaagata caagaaaatt
     2341 tagctgattt agcaacagtt gattttgact ctttagagtt cagagaaaag caactttatt
     2401 taaaatcact aataaacaaa atttatattg atggtgaaca agttactatt gaatggctcg
     2461 aacagaaact gatcagcgaa gaagatctgt gatagtaata acccgggggt tcccatggta
     2521 cgcgtgctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     2581 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagatact
     2641 agtagcggcc gctgcaggag tcactaaggg ttagttagtt agattagcag aaagtcaaaa
     2701 gcctccgacc ggaggctttt gactaaaact tcccttgggg ttatcattgg ggctcactca
     2761 aaggcggtaa tcagataaaa aaaatcctta gctttcgcta aggatgattt ctgctagtat
     2821 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     2881 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcttatttgc
     2941 cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc
     3001 gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg
     3061 gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga
     3121 ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat
     3181 cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata
     3241 gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc
     3301 tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga
     3361 agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg
     3421 gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa
     3481 tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg
     3541 ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca
     3601 ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc
     3661 gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc
     3721 tcatacgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga
     3781 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
     3841 gcgccccagc tggcaattcc ggttcgcttg ctctctagta gttacattgt cgatctgttc
     3901 atggtgaaca gctttgaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt
     3961 tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatgtaac ggtgaacagt
     4021 tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac
     4081 ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga
     4141 gccatgagaa cgaaccattg agatcatact tactttgcat gtcactcaaa aattttgcct
     4201 caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt
     4261 tatgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct
     4321 ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca
     4381 acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta
     4441 aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat
     4501 tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag
     4561 ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt
     4621 caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag
     4681 gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg
     4741 tccactggaa aatcccaaag cctttaacca aaggattcct gatttccaca gttctcgtca
     4801 tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca
     4861 tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa
     4921 tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt
     4981 catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca
     5041 attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac
     5101 atgtcctttt cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac
     5161 ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt
     5221 atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag
     5281 atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt
     5341 cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca
     5401 ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg
     5461 agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg
     5521 ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa
     5581 gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc
     5641 tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt
     5701 atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac
     5761 aggcttaccc gtcttactgt ccctagtgct tggattctca ccaataaaaa acgcccggcg
     5821 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     5881 caggagtcca agcgagctcg taaacttggt ctgacagctc tagctccggc aaaaaaacgg
     5941 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgtttgcc
     6001 acctgacgtc taagaaaagg aatattcagc aatttgcccg tgccgaagaa aggcccaccc
     6061 gtgaaggtga gccagtgagt tgattgctac gtaattagtt agttagccct tagtgactcg
     6121 aattcgcggc cgcttctaga g
//
