LOCUS       sequence-426215-       22675 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Binary vector expressing the Lineage Tracing system. Cas9 expression
            driven by M. polymorpha EF1 promoter. LT1 reporter under control of 
            MpEF1p.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22675)
  AUTHORS   Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M,
            Dolan L, Mittelsten Scheid O
  TITLE     A versatile CRISPR-based system for lineage tracing in living 
            plants.
  JOURNAL   Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378.
  PUBMED    37403571
REFERENCE   2  (bases 1 to 22675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2023 Jul 5. doi: 10.1111/tpj.16378."
COMMENT     Sequence Label: pEF1Cas-LT1
FEATURES             Location/Qualifiers
     source          1..22675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    214..238
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     291..313
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5986..10089
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5986..10089
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10102..10122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_RNA        11111..11186
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(13409..13411,13412..13414,13415..14125)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(13454..13475)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     14062..14083
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             15494..16522
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      16530..17237
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(17270..17288)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(17271..17288)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    17301..17348
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
     misc_feature    17460..17484
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      17564..18195
                     /label=oriV
                     /note="incP origin of replication"
     CDS             19606..20400
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             20699..21847
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(22087..22675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(22167..22186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt ggatctcctg
      121 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
      181 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
      241 cactgatagt ttaaactgaa ggcgggaaac gacaatctgc tagtggatct cccagtcacg
      301 acgttgtaaa acgggcgccc cgcggaaagc ttgctagcca attggggccc aacgttctcg
      361 agtttttcta gaaggccttg gatccacccg ggagaattcg tcgactttgc ggccgcatcg
      421 atactgcagg agctcggtac cttttactag tgatatcgat catgagcgac ctcgtcacca
      481 catgaacctc acctcctcat ttaaaatagt ataatgctgg attgattgaa gaattagaca
      541 taattctttt gcgcatccct ttatgttttg ttttcatttt ttgggcgatg tcagttaacg
      601 cggtggtatt gcatggcggc tacatgcaag tacattcata gtttaactgg agcgaggcct
      661 ttcacacgtg tcctcccttt aaaacaacgg ctttgaaagt aagtccgtgt cgccacacaa
      721 ttcggctttc ccagcccaag cctcgaatca tgaggactac gaacagacac acacagtcca
      781 gcgagcgcga tcgagccttc tcgctctctc tctctcccct ctcgctggct cttctgccct
      841 ggggccgatg gaacgaacga acgagcccac ctttctcctc tctctctctc tctctctctc
      901 tcctcgctca ctggccacgt cagtggatgc agaggctggg ggcggctgga atcgctcgat
      961 cgaccaccgg ccgtcaccct cgctacgaac cccgccggcc cgaccctggc cccgtggttc
     1021 cgctctctcg tcgacccggt ccacggcccc ggtcccgttg tgaaatgaat caatccgctc
     1081 gcccgctgcc ccgaggtcag tcaggtctgg tacgggcctg gtattctcgg cgatgtactc
     1141 tgactggcag tcgttcgttt gtgggccgcg gggggatata ctggagagag aaagagatgg
     1201 agagagggag agagagggag ggatgggaga gagagagaga ggtcaaagag agccttttgg
     1261 ttggagtaaa tgagattgtc cgttttccgt tacaacagca ggcggagagg gatcgagaga
     1321 gcatcattga gatgaggtgc cgggacggag tgtagtgaat tgaatcgact cgagtcgaat
     1381 tgaatcgatg gcattgattc gagggcgctt gggatgattc gctgccgttg ggggttgggg
     1441 ttcacgtagt gctctcgaac tggaaaccag tccgcaacag tgtctcacat attccccact
     1501 aggcgcgacg atggatggag aaaaatgcga ggggcccggc cccaggcgca ggactacaca
     1561 cgtccagttg tcttgtctcg gcagacattt gctcactcag tgatggagag cctgtaacgt
     1621 cagggctcct gatgtcacgc ctgcccgcat cgcatgagac aaaggcggga gtcctcacgc
     1681 gcatcttttc gcccctccga tctcagtcgc ctttggctca gctccccgcc cctaatttcc
     1741 attaccaact aaccacgaag tcgggcccgg gccgcgggcc cagatccata gatcctccct
     1801 cccttccatg tggattctcc gagccaatat acagtttggc aatatatatg tctgtatttc
     1861 aaagagggcc gcacatgaga ggccaaaatt ggttaaaata cagccttttg attccccagc
     1921 tccagagcca tcgagtcagg ttgcacatgg gtctttcgag gtccgtgcac ggaggtgatc
     1981 cgcctctgag atcagtcatt gtacagcctc ggacctccag cgcgagagag tgcgaagtgg
     2041 ccgcgagcag tagcagtagc agcagcagca gcggcgtagg agtacgagga tagggatgac
     2101 attcgatcgg tttcgctaac ggaacgggac gaaaagaagg gacgagatcg acaccaaaag
     2161 caatccaaag caaaggaatg gaaagcgaga agaagaaggc cgcccacttc tttcgctttc
     2221 gaggcctcct cgtcctctcg tctgcaccac caccgcagcg agcgaggtcc ttcctccctt
     2281 ggccctcctc gagcctacgt ttggcccagc cccgtaggcc aaacgaaaga actatccatc
     2341 catccttcct tccttcccat ccatccccgt tggcactcag gcaggcaggc aggcaggcaa
     2401 aagcggcagc tacggcctct tgcatgaaag aatgtatcat cttttgccag tcgcaggtcg
     2461 tcggcctcca ccgccggccc tccttcctct ccgggcagcg ccggaaagct agagtttgac
     2521 gcgcccgtgg cccgcctcgc cggtgatgcc caaggcgccg cgcatatccg aacgtttcgg
     2581 ttgcgacgga ccgtccatcg ggatggggct caataaagaa ggcagcaaag gaaggaaagg
     2641 aatggaaagg aaaggaaagg aagggaaggt gcggtggtac agcgtaatgt gctcggccga
     2701 gaggtatcgg caacagtaaa agagaatgtt tcagaacact gtgggaaaag gcacgaagga
     2761 aggaaggaag gaggcatggg atgggatggc atgaggcatg gggcatgggg catggctggg
     2821 ctctcatgcg ttcgtcgcgc ctgcagcaga tagagctcgc agagcgtcgc caccgggaca
     2881 cacgaacgag cgcacatccg gaaaagcctt ctccagggat tcatgatcgg ggctcttgga
     2941 cgaccttcgc agcaatgctc ggacccaatc acccggagct ctgatcctcc ccgcccctcg
     3001 cctcgccggg atgcgtcgcc tctcgctttc tgtcacggct ggcactgcag ggcttgcttt
     3061 ttcgcagtgt gcttttctgg cgggcctcct ttttctgtaa gcctcttccg acagagggag
     3121 ggagcggaga cgaaaagaag aagcaacgag cggaagatac tgtacgtgtc ctcgttctcc
     3181 ctcggacgtc ccgtcaccat cgagcgagga ggacaaacgg gcgaaccggt tgacatccgc
     3241 aaccttgtcc gggccgcggg cgagctcgca gcgatgcggg cgtttggtgc cgacattcat
     3301 ccttattaat cctcgacgca gccgcgaatg ccatcgacca caagacgtta agcgaaaatg
     3361 atgcgaacgc aattcttgca agatatcgtt ttgcaggccg aattaaagcc gttggaatga
     3421 tgacttaaat gccccctttt attagtagaa agtcaggttg gcggaattat gaggtaggtg
     3481 gtgactgggc gatcggcgaa ggattgcaag ttgatttcgt ttgatgcgag cggattcatt
     3541 cggtgagaat aagctgcgca tttggaagtg gctgcacatg ataccgacga tttgaggcag
     3601 acgggcgaac ggggatcagg cgaatgagca tgagtgcagc agccctcgcc ctccactcca
     3661 ctccactcca cttcactccc gctttggaac ggtcatcaat taagcgatac tgctcgagcc
     3721 caaatctgcc gacgagccgg atttctaaat catgatttcc cgagatgagt gagaaaatta
     3781 tcaacctcta tgggaatgat cacagctaag ctaatcgcta gactagacgt ccgagggaat
     3841 tgatcattta tttcgcgtgc aattttataa gtttacttga gtggtgcctc tggttcgaaa
     3901 accataaaaa gaaaggaggg aaacatttgc cagcttaaaa ggtaccacat ttggccccgg
     3961 tgtacgattc ttgttgaaga atacctcgga cgggttctgt tcggttctgt tccaagatac
     4021 gatatggagc cgctccaaaa gcctgccatg attgttcaag ttggagctag gagaaaagcg
     4081 ttgctactag tctaaaggcc tcaaccaact atcacccaaa ccccgattca ttgcgtttct
     4141 aaaggattac tttcccaaaa gaaaaccccg gaaatcacat ccttcctttc cggattgaaa
     4201 tatacaaact cccccctccc cctccccacc cccacatgtc ctgcccatcg gactccagcg
     4261 ggcaagcacg cttgagcatc ctcgaggccc tatcccatcg cgacgacgcc cctcctctcc
     4321 cctgctcatc gagagctcgg agcccaaggg cgtgtcggag agaggcccgc aaaattcgcc
     4381 ctcaaaattc gcccgaaaac gaagctctgc tctcttccct gctgtctgtg gtctgctctc
     4441 ttctctgctt tctatggcgc cccgataagg agcgagggcg gcccgctcga tcggtgaccg
     4501 cccttcggtc gcaacgcaaa gtccagaaat gccccaaata cgtggacctt gggggcgagc
     4561 cgctcaggta gagttaaaat ataatttttc aggggaatga aatgaggacc aggtgtcgac
     4621 atatcagtgg gcttatattc aagatgttct ggcctaaaac cttgaggtat aaaggcgagg
     4681 acaagagttt ttgtccacga taagatttct ctgacattcc gatatccctt ttcggcccct
     4741 aataacctgc gctgtcaccg ggaaagcggg ccacaatttc ccggccggaa acatgggcca
     4801 aacacctccc tccctccctc cctccctccc gtcgccctgc gcgaccatcc ccggcaggca
     4861 agccgcgggc ctaggcctct agcggtactg taccccccag gcctagaacc gtaccctgag
     4921 tgcccagaga gaacagcaga ggcctgcagg tcaggtcagg tcaggtcagg gcaggcaatg
     4981 gcagcgaagg gcaggagcgg accctttcgg gtttggaccg ctggatgcga aactgccggg
     5041 aacgtccgct gccgagggag aaagtgaaaa tccggcgccg ggctcgctcg ggctgaaata
     5101 cgtgtgccgt ggtaatcatc aagaccatca gcacgcagca gtgtcgagcc aaacgctctg
     5161 cgcttccccc acccccgccc attcaagtag acgccgctct cgtcccgttt aggcctattc
     5221 caccggactg ggctcgcatc ctctccttcg ctcgatcccc tcccctcccc acctctgggc
     5281 caaaaccaca aagccttcag accctccgcc ctcagacttc gctgctccac ctccactact
     5341 acatcgctct cgtcgacctc gtcctcgcat tccgctcgct tccaatcctt tagggctcga
     5401 gccctcccat cctggaggga cttcctgtac tctcctctct cttctttgcc aagcgccacc
     5461 tttttctccc gtgtacttgc gacctcctcc tcgcattcag cctccggacg ttctgttctc
     5521 ttcttcacgc cttgcgcctc gtgtctttcg taaattctct ccgaccgacc agcgcggtgt
     5581 ttgcttatcc gtctttcccg actctctctc gcaggctctt gtttagaatc tgactgctcg
     5641 tcatttgcgg tttgcgcttc tggccccgga gcgtcgtcgt acacagctta tgctccgtgc
     5701 aaatctgtgg tgaaatctgg agaagatctg cacccgaatt ttctcgctta attgtccgtc
     5761 gggctgctgt gggtcgagcg cagcgagtag tgcgtcctct gtagagctgt accgggcgga
     5821 aaatgtgctc cacgattcac attgatttgt gatatataaa gtcagcgttg tgcgggcttg
     5881 cggctgtaat tgtgtgattg gagcctctga gattggcaac gttgagagtc ttcaggagaa
     5941 gaggcggacc cgaagacatc aatttgctga tttcgtcgac aaacaatgga taagaagtac
     6001 tctatcggac tcgatatcgg aactaactct gtgggatggg ctgtgatcac cgatgagtac
     6061 aaggtgccat ctaagaagtt caaggttctc ggaaacaccg ataggcactc tatcaagaaa
     6121 aaccttatcg gtgctctcct cttcgattct ggtgaaactg ctgaggctac cagactcaag
     6181 agaaccgcta gaagaaggta caccagaaga aagaacagga tctgctacct ccaagagatc
     6241 ttctctaacg agatggctaa agtggatgat tcattcttcc acaggctcga agagtcattc
     6301 ctcgtggaag aagataagaa gcacgagagg caccctatct tcggaaacat cgttgatgag
     6361 gtggcatacc acgagaagta ccctactatc taccacctca gaaagaagct cgttgattct
     6421 actgataagg ctgatctcag gctcatctac ctcgctctcg ctcacatgat caagttcaga
     6481 ggacacttcc tcatcgaggg tgatctcaac cctgataact ctgatgtgga taagttgttc
     6541 atccagctcg tgcagaccta caaccagctt ttcgaagaga accctatcaa cgcttcaggt
     6601 gtggatgcta aggctatcct ctctgctagg ctctctaagt caagaaggct tgagaacctc
     6661 attgctcagc tccctggtga gaagaagaac ggacttttcg gaaacttgat cgctctctct
     6721 ctcggactca cccctaactt caagtctaac ttcgatctcg ctgaggatgc aaagctccag
     6781 ctctcaaagg atacctacga tgatgatctc gataacctcc tcgctcagat cggagatcag
     6841 tacgctgatt tgttcctcgc tgctaagaac ctctctgatg ctatcctcct cagtgatatc
     6901 ctcagagtga acaccgagat caccaaggct ccactctcag cttctatgat caagagatac
     6961 gatgagcacc accaggatct cacacttctc aaggctcttg ttagacagca gctcccagag
     7021 aagtacaaag agattttctt cgatcagtct aagaacggat acgctggtta catcgatggt
     7081 ggtgcatctc aagaagagtt ctacaagttc atcaagccta tcctcgagaa gatggatgga
     7141 accgaggaac tcctcgtgaa gctcaataga gaggatcttc tcagaaagca gaggaccttc
     7201 gataacggat ctatccctca tcagatccac ctcggagagt tgcacgctat ccttagaagg
     7261 caagaggatt tctacccatt cctcaaggat aacagggaaa agattgagaa gattctcacc
     7321 ttcagaatcc cttactacgt gggacctctc gctagaggaa actcaagatt cgcttggatg
     7381 accagaaagt ctgaggaaac catcacccct tggaacttcg aagaggtggt ggataagggt
     7441 gctagtgctc agtctttcat cgagaggatg accaacttcg ataagaacct tccaaacgag
     7501 aaggtgctcc ctaagcactc tttgctctac gagtacttca ccgtgtacaa cgagttgacc
     7561 aaggttaagt acgtgaccga gggaatgagg aagcctgctt ttttgtcagg tgagcaaaag
     7621 aaggctatcg ttgatctctt gttcaagacc aacagaaagg tgaccgtgaa gcagctcaaa
     7681 gaggattact tcaagaaaat cgagtgcttc gattcagttg agatttctgg tgttgaggat
     7741 aggttcaacg catctctcgg aacctaccac gatctcctca agatcattaa ggataaggat
     7801 ttcttggata acgaggaaaa cgaggatatc ttggaggata tcgttcttac cctcaccctc
     7861 tttgaagata gagagatgat tgaagaaagg ctcaagacct acgctcatct cttcgatgat
     7921 aaggtgatga agcagttgaa gagaagaaga tacactggtt ggggaaggct ctcaagaaag
     7981 ctcattaacg gaatcaggga taagcagtct ggaaagacaa tccttgattt cctcaagtct
     8041 gatggattcg ctaacagaaa cttcatgcag ctcatccacg atgattctct cacctttaaa
     8101 gaggatatcc agaaggctca ggtttcagga cagggtgata gtctccatga gcatatcgct
     8161 aacctcgctg gatctcctgc aatcaagaag ggaatcctcc agactgtgaa ggttgtggat
     8221 gagttggtga aggtgatggg aaggcataag cctgagaaca tcgtgatcga aatggctaga
     8281 gagaaccaga ccactcagaa gggacagaag aactctaggg aaaggatgaa gaggatcgag
     8341 gaaggtatca aagagcttgg atctcagatc ctcaaagagc accctgttga gaacactcag
     8401 ctccagaatg agaagctcta cctctactac ctccagaacg gaagggatat gtatgtggat
     8461 caagagttgg atatcaacag gctctctgat tacgatgttg atcatatcgt gccacagtca
     8521 ttcttgaagg atgattctat cgataacaag gtgctcacca ggtctgataa gaacaggggt
     8581 aagagtgata acgtgccaag tgaagaggtt gtgaagaaaa tgaagaacta ttggaggcag
     8641 ctcctcaacg ctaagctcat cactcagaga aagttcgata acttgactaa ggctgagagg
     8701 ggaggactct ctgaattgga taaggcagga ttcatcaaga ggcagcttgt ggaaaccagg
     8761 cagatcacta agcacgttgc acagatcctc gattctagga tgaacaccaa gtacgatgag
     8821 aacgataagt tgatcaggga agtgaaggtt atcaccctca agtcaaagct cgtgtctgat
     8881 ttcagaaagg atttccaatt ctacaaggtg agggaaatca acaactacca ccacgctcac
     8941 gatgcttacc ttaacgctgt tgttggaacc gctctcatca agaagtatcc taagctcgag
     9001 tcagagttcg tgtacggtga ttacaaggtg tacgatgtga ggaagatgat cgctaagtct
     9061 gagcaagaga tcggaaaggc taccgctaag tatttcttct actctaacat catgaatttc
     9121 ttcaagaccg agattaccct cgctaacggt gagatcagaa agaggccact catcgagaca
     9181 aacggtgaaa caggtgagat cgtgtgggat aagggaaggg atttcgctac cgttagaaag
     9241 gtgctctcta tgccacaggt gaacatcgtt aagaaaaccg aggtgcagac cggtggattc
     9301 tctaaagagt ctatcctccc taagaggaac tctgataagc tcattgctag gaagaaggat
     9361 tgggacccta agaaatacgg tggtttcgat tctcctaccg tggcttactc tgttctcgtt
     9421 gtggctaagg ttgagaaggg aaagagtaag aagctcaagt ctgttaagga acttctcgga
     9481 atcactatca tggaaaggtc atctttcgag aagaacccaa tcgatttcct cgaggctaag
     9541 ggatacaaag aggttaagaa ggatctcatc atcaagctcc caaagtactc actcttcgaa
     9601 ctcgagaacg gtagaaagag gatgctcgct tctgctggtg agcttcaaaa gggaaacgag
     9661 cttgctctcc catctaagta cgttaacttt ctttacctcg cttctcacta cgagaagttg
     9721 aagggatctc cagaagataa cgagcagaag caacttttcg ttgagcagca caagcactac
     9781 ttggatgaga tcatcgagca gatctctgag ttctctaaaa gggtgatcct cgctgatgca
     9841 aacctcgata aggtgttgtc tgcttacaac aagcacagag ataagcctat cagggaacag
     9901 gcagagaaca tcatccatct cttcaccctt accaacctcg gtgctcctgc tgctttcaag
     9961 tacttcgata caaccatcga taggaagaga tacacctcta ccaaagaagt gctcgatgct
    10021 accctcatcc atcagtctat cactggactc tacgagacta ggatcgatct ctcacagctc
    10081 ggtggtgatt caagggctga tcctaagaag aagaggaagg tttgaggggc tcacgacgaa
    10141 ctctaagagc tcaggcctcc cagctttcgt ccgtatcatc ggtttcgaca acgttcgtca
    10201 agttcaatgc atcagtttca ttgcccacac accagaatcc tactaagttt gagtattatg
    10261 gcattggaaa agctgttttc ttctatcatt tgttctgctt gtaatttact gtgttctttc
    10321 agtttttgtt ttcggacatc aaaatgcaaa tggatggata agagttaata aatgatatgg
    10381 tccttttgtt cattctcaaa ttattattat ctgttgtttt tactttaatg ggttgaattt
    10441 aagtaagaaa ggaactaaca gtgtgatatt aaggtgcaat gttagacata taaaacagtc
    10501 tttcacctct ctttggttat gtcttgaatt ggtttgtttc ttcacttatc tgtgtaatca
    10561 agtttactat gagtctatga tcaagtaatt atgcaatcaa gttaagtaca gtataggctt
    10621 tcagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag cttacatttt
    10681 cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt ttgtatcttg
    10741 tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga atttgattga
    10801 ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct gggaatctga
    10861 aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta tataggccca
    10921 tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa cgaagctgag
    10981 tttatataca gctagagtcg aagtagtgat tggaacaaag caccagtggt ctagtggtag
    11041 aatagtaccc tgccacggta cagacccggg ttcgattccc ggctggtgca gtcccctcca
    11101 ccccacagtg gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
    11161 ttgaaaaagt ggcaccgagt cggtgctttt tttttttttc tgccaaatga gtcacacaca
    11221 ttgttgagag acatatcaaa ataactcaat tgtaaggtaa gttgatagat ttcccagtta
    11281 aatgcattat tttaacaata tttgtatctc aaattttcaa aatcatatca aatttgcaga
    11341 tcttgatgat gattcacaac tctgtcttcg atttttgatt gttaacgggt atcgatattt
    11401 gggtaactta aaaagaattt gctaaagatc ttaaaaaaca gattaaaaaa acctttcaaa
    11461 atagctaatc ctttaaatcc gtttcacaat ataatgctca ttgatgataa tcgaaaagat
    11521 tccattttaa agtgcatata aattgcttaa ttatctcttt ctgtctcgta atattttgtt
    11581 ctctcctatt ggatcggacc cgcatcttct cgatcagttc actatactaa tatttggtca
    11641 acttcaagag agattagaac ttcgttctcc aaagtgtaaa ggccgacaag cagaaaagga
    11701 aacttcaatc agaagccttc taggttttcc cgcgtcccaa aaagcgaagg gaggacgggg
    11761 aggacggtga ggccagaaga agaaagcaac agtaaaatct catgtgattg cgtgcatgtc
    11821 atggatctat gggatgctag tccaccgttt ggaagtatgt gcagaaggag aaaggcgcgg
    11881 gaggccaaaa gcgacttcgg ccacctctct actttctaga aagtttgctc gcagcttgta
    11941 ataagcgcgg aaaaggtact cgaaaaaccg gagcaatcgg tgtggtatca gcagtcgggt
    12001 tcgctaaacc tacgtcttct tattttggaa tgttaaattg aaagggcgtg ccggccctaa
    12061 gcacggacga tgtgacagac ggaggagtgt gagcaattcg gatgacgatg ctggccggca
    12121 gtcacgacaa agagtccgaa ggggcatgga cagggcaata tataaatgga aaagaggctt
    12181 tgaatattcc taacccttcg tcgtcggcct cccaacttgc cctcgcatgc tgaattggcc
    12241 gcctatctgc gaatggccgg ccgccgaccc taagtcgcaa taacttgaac ggagccgatg
    12301 caaagtcctt atcgaacgct cgttattata aacggggcag acctaggtat ttaattatat
    12361 caaaaccctc cgcaatttcg ctaatcattc cctaatttct tctactttgg cgtctagtgc
    12421 tgtggtcgcg cttgctctca aagctcgctg ggaggcagtg gagcgtctgg cttaaaatcg
    12481 atcgccgcaa ccctagagtt cctttcactt ctttctctct tgcgtggaga gcatcgacat
    12541 cgaggagctg ctacttgtct tctggtatgc gccttgtgcc ctcgtctctg tacgtcgtta
    12601 cgattgctcg atattgtgat tgtctagacc tgaaacccga ctggagcttt tacgtggtgt
    12661 cgattggggc ctcgagcgag tgatttttta ggttcgtttt agccaagtac atcccacttt
    12721 ggtcagtcct gttatctcgg taaggtgttc agttcggtca ctggtgcgga ggtgcctcgt
    12781 aagtttcctg catgatagat ttgtctatcc ttgacttgaa gaggagtcga aggtcagcga
    12841 ggttggctgt gtgctatgga cgtcggtgct ccttggttcc gtgcttctat gggtgttgcc
    12901 tcaacagtat tgatgtgcct gcagaaaggt tgtcaccgac gatggtcccc tccaccccac
    12961 agtgggggca ccaagagccg agaagaagcc cgccgagaaa aaaaccgccg ctgagaggcc
    13021 ggtggaagag aacaaggctg ctgaaaaggc tccggcggag aagaaaccca aggcggggaa
    13081 gaaattgcca ccaaaggaag ccggtgacaa gaagaagaag cgatctaaga aaaacgtcga
    13141 aacctacaag atctacatct tcaaggtgct gaagcaggtt catccagata tcggaatttc
    13201 aagcaaagcc atggggatta tgaacagttt catcaacgac atattcgaga agctagctca
    13261 ggaatcttcg aaactcgcta ggtacaacaa gaagccgacc attacatctc gcgagatcca
    13321 gacggctgtg agactcgtcc tccccggtga actcgctaaa cacgccgtct ctgaaggtac
    13381 caaggcggtt accaagttta ctagctccat ggtgagcaag ggcgaggagc tgttcaccgg
    13441 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtccg
    13501 cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac
    13561 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcgtggcctg
    13621 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
    13681 aggctacgtc caggagcgca ccatctcttt caaggacgac ggtacctaca agacccgcgc
    13741 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
    13801 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaacgt
    13861 ctatatcacg gccgacaagc agaagaacgg catcaaggct aacttcaaga tccgccacaa
    13921 cgttgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
    13981 cggccccgtg ctgctgcccg acaaccacta cctgagccat cagtccgccc tgagcaaaga
    14041 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggattac
    14101 acatggcatg gacgagctgt acaagtaatc aggtggatcc tagataacct ttaccttcat
    14161 tccctgtaaa ttggtaccct gcatctcgtc tctgttatgc ttaagaagtt caatgtttcg
    14221 tttcatgtaa aactttggtg gtttgtgttt tggggccttg tataatccct gatgaataag
    14281 tgttctacta tgtttccgtt cctgttatct ctttctttct aatgacaagt cgaacttctt
    14341 ctttatcatc gcttcgtttt tattatctgt gcttcttttg tttaatacgc ctgcaaagtg
    14401 actcgactct gtttagtgca gttctgcgaa acttgtaaat agtccaattg ttggcctcta
    14461 gtaatagatg tagcgaaagt gttgagctgt tgggttctaa ggatggcttg aacatgttaa
    14521 tcttttaggt tctgagtatg atgaacattc gttgttgcta agaaatgcct gtaatgtccc
    14581 acaaatgtag aaaatggttc gtacctttgt ccaagcattg atatgtctga tgagaggaaa
    14641 ctgcaagata ctgagcttgg tttaacgaag gagaggcagt ttcttccttc caaagcattt
    14701 catttgacaa tgccttgatc atcttaagta gagtttctgt tgtggaaagt ttgaaacttt
    14761 gaagaaacga ctctcaagta aattgatgat cacaagtgaa agtgtatgtt actaactagt
    14821 cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt
    14881 atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca
    14941 actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc
    15001 gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca
    15061 atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac
    15121 tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa
    15181 atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg
    15241 ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc ttaattctcg
    15301 attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt
    15361 actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa
    15421 tttgtagatt aatctgagtt tttctgatta acaccacacc aatctggtac cagaaaaggg
    15481 gagaagcaaa accatgggta aaaagcctga actcaccgcg acgtctgtcg agaagtttct
    15541 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
    15601 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
    15661 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
    15721 ggaagtgctt gacattgggg cattcagcga gagcctgacc tattgcatct cccgccgtgc
    15781 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcaaccggt
    15841 cgcggaggcg atggatgcca tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
    15901 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
    15961 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
    16021 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
    16081 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
    16141 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
    16201 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
    16261 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
    16321 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
    16381 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
    16441 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
    16501 tcgtccgagg gcaaaggaat agatgcatcc tgctttaatg agatatgcga gacgcctatg
    16561 atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta
    16621 gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg
    16681 tatttttatg aataatattc tccgttcaat ttactgattg taccctacta cttatatgta
    16741 caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag
    16801 agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg
    16861 cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc
    16921 ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac
    16981 tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc
    17041 tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact
    17101 tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag
    17161 gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac
    17221 atgtcgaggc tcagcaggac cggcatgcaa gctagcttac tagtgatatt ctatagtgtc
    17281 acctaaatgt atgatctagg gaagttccta ttccgaagtt cctattctct gaaaagtata
    17341 ggaacttctt tgcgtattgg gcgctcttgg cctttttggc caccggtcgt acggttaaaa
    17401 ccaccccagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
    17461 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
    17521 acaaaatcac cactcgatac aggcagccca tcagtccact agagacgctc accgggctgg
    17581 ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt
    17641 cgaagccgtg tgcgagacac cgcagccgcc ggcgttgtgg atacctcgcg gaaaacttgg
    17701 ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg
    17761 cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc
    17821 aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg
    17881 ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc
    17941 gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg
    18001 aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc
    18061 accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc
    18121 agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa
    18181 ccctcccggc ccgctctcgc gttggcagca tcacccataa ttgtggtttc aaaatcggct
    18241 ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt
    18301 atttaaagtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattaggga
    18361 aggtgcgaat aagcggggaa attcttctcg gctgactcag tcatttcatt tcttcatgtt
    18421 tgagccgatt ttttctcccg taaatgcctt gaatcagcct atttagaccg tttcttcgcc
    18481 atttaaggcg ttatccccag tttttagtga gatctctccc actgacgtat catttggtcc
    18541 gcccgaaaca ggttggccag cgtgaataac atcgccagtt ggttatcgtt tttcagcaac
    18601 cccttgtatc tggctttcac gaagccgaac tgtcgcttga tgatgcgaaa tgggtgctcc
    18661 accctggccc ggatgctggc tttcatgtat tcgatgttga tggccgtttt gttcttgcgt
    18721 ggatgctgtt tcaaggttct taccttgccg gggcgctcgg cgatcagcca gtccacatcc
    18781 acctcggcca gctcctcgcg ctgtggcgcc ccttggtagc cggcatcggc tgagacaaat
    18841 tgctcctctc catgcagcag attacccagc tgattgaggt catgctcgtt ggccgcggtg
    18901 gtgaccaggc tgtgggtcag gccactcttg gcatcgacac caatgtgggc cttcatgcca
    18961 aagtgccact gattgccttt cttggtctga tgcatctccg gatcgcgttg ctgctctttg
    19021 ttcttggtcg agctgggtgc ctcaatgatg gtggcatcga ccaaggtgcc ttgagtcatc
    19081 atgacgcctg cttcggccag ccagcgattg atggtcttga acaattggcg ggccagttga
    19141 tgctgctcca gcaggtggcg gaaattcatg atggtggtgc ggtccggcaa ggcgctatcc
    19201 agggataacc gggcaaacag acgcatggag gcgatttcgt acagagcatc ttccatcgcg
    19261 ccatcgctca ggttgtacca atgctgcatg cagtgaatgc gtagcatggt ttccagcgga
    19321 taaggtcgcc ggccattacc agccttgggg taaaacggct cgatgacttc caccatgttt
    19381 tgccatggca gaatctgctc catgcgggac aagaaaatct cttttctggt ctgacggcgc
    19441 ttactgctga attcactgtc ggcgaaggta agttgatgac tcatgatgaa ccctgttcta
    19501 tggctccaga tgacaaacat gatctcatat cagggacttg ttcgcacctt ccttagcttc
    19561 ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga
    19621 atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga
    19681 atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg
    19741 acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta
    19801 tggctggaag gaaagctgcc tgttccaaag gtcctgcacc ttgaacggca tgatggctgg
    19861 agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa
    19921 caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc
    19981 gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac
    20041 ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga caccccattt
    20101 aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc
    20161 ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc
    20221 tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc
    20281 cggtcgatca gggaggatat tggggaagaa cagtatgtcg agctattttt tgacttactg
    20341 gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga attgttttag
    20401 tacctagatg tggcgcaacg atgctggcga caagcaggag cgcaccgact tcttccgcat
    20461 caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg ggtcgctggt
    20521 attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga cggtctacgg
    20581 gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag gcggatcaaa
    20641 tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag gagggtgaat
    20701 gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg ggttttccgc
    20761 cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca
    20821 gtccgtcggc tcgatggccc agcaagctac ggccaagatc gagcgcgaca gcgtgcaact
    20881 ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc gtctcgaaca
    20941 ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta tgacgaccaa
    21001 gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca agcaagccgc
    21061 gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt tcgatattgc
    21121 gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg ccctgttcac
    21181 cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa
    21241 caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg acgaactggt
    21301 gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga tcaccttcac
    21361 gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt acacgaaggc
    21421 cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg
    21481 gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac
    21541 gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta
    21601 cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac ggatgttcga
    21661 ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg
    21721 cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga
    21781 gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa
    21841 acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg ctttactgag
    21901 atcctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    21961 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    22021 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    22081 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    22141 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    22201 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    22261 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    22321 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    22381 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    22441 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    22501 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    22561 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    22621 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaa
//
