LOCUS       sequence-426301-        9688 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of mutated alpha-synuclein (2nd amino acid 
            aspartate mutated to arginine) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Santhosh Kumar S, Naseri NN, Pather SR, Hallacli E, Ndayisaba A, 
            Buenaventura C, Acosta K, Roof J, Fazelinia H, Spruce LA, Luk K, 
            Khurana V, Rhoades E, Shalem O
  TITLE     Sequential CRISPR screening reveals partial NatB inhibition as a 
            strategy to mitigate alpha-synuclein levels in human neurons.
  JOURNAL   Sci Adv. 2024 Feb 9;10(6):eadj4767. doi: 10.1126/sciadv.adj4767. 
            Epub 2024 Feb 9.
  PUBMED    38335281
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-9"; volume: "10(6):eadj4767. doi"; pages: " 
            10.1126/sciadv.adj4767. Epub 2024 Feb 9"
COMMENT     Sequence Label: FUW-hSNCA(D2R)-NE
FEATURES             Location/Qualifiers
     source          1..9688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(28..47)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        219..598
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        600..798
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     753..773
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             816..996
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1043..1168
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1661..1894
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2079..2123
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2421..2538
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2603..3814
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3651..3670
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    4361..4949
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4414..4434)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4832..4843)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4952..4968)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4953..4969)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5474..5654
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5680..5697)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5686..5910
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5956..6384
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6043..6062)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6253..6274
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6393..6413)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6398..6727
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6578..6713
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6640..6659
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6775..6822
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6841..7215
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7345..7466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7382..7401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7436..7455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7515..7531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7515..7531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7528..7550)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7539..7555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7563..7569,7570..7587,7588..7593))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7608..7629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7746..7763)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7917..8505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7997..8016)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8676..9467,9468..9536))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9299..9318
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9537..9641)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
       61 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
      121 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
      181 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      241 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      301 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      361 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      421 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      481 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      541 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      601 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      661 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      721 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      781 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
      841 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      901 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      961 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     1021 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     1081 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1141 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1201 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1261 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1321 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1381 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1441 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1501 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1561 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1621 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1681 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1741 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1801 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1861 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1921 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1981 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     2041 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     2101 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     2161 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2221 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2281 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2341 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     2401 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2461 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2521 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     2581 aacccgtgtc ggctccagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
     2641 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
     2701 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
     2761 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
     2821 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
     2881 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
     2941 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
     3001 gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
     3061 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
     3121 gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag
     3181 acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa
     3241 gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc
     3301 tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt
     3361 cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg
     3421 gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg
     3481 gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg
     3541 cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt
     3601 gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg
     3661 gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt
     3721 tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc
     3781 taaattctgg ccgtttttgg cttttttgtt agacgaagct tgggctgcag gtcgactcta
     3841 gagccaccat gcgtgtattc atgaaaggac tttcaaaggc caaggaggga gttgtggctg
     3901 ctgctgagaa aaccaaacag ggtgtggcag aagcagcagg aaagacaaaa gagggtgttc
     3961 tctatgtagg ctccaaaacc aaggagggag tggtgcatgg tgtggcaaca gtggctgaga
     4021 agaccaaaga gcaagtgaca aatgttggag gagcagtggt gacgggtgtg acagcagtag
     4081 cccagaagac agtggaggga gcagggagca ttgcagcagc cactggcttt gtcaaaaagg
     4141 accagttggg caagaatgaa gaaggagccc cacaggaagg gattctggaa gatatgcctg
     4201 tggatcctga caatgaggct tatgaaatgc cttctgagga agggtatcaa gactacgaac
     4261 ctgaagccac caaagaaaac ccgcgtagca accaggaaga aagctatgat gataacgaaa
     4321 gctaaggatc cgttaacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     4381 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     4441 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     4501 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     4561 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     4621 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     4681 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     4741 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     4801 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     4861 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     4921 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     4981 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     5041 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     5101 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5161 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     5221 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     5281 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     5341 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     5401 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     5461 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5521 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5581 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5641 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     5701 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5761 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5821 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     5881 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     5941 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6001 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6061 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6121 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6181 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6241 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6301 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6361 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     6421 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6481 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6541 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6601 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6661 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6721 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     6781 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     6841 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     6901 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     6961 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     7021 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     7081 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     7141 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     7201 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     7261 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     7321 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     7381 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7441 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     7501 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7561 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7621 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7681 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7741 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7801 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7861 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7921 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7981 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8041 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8101 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8161 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8221 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8281 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8341 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8401 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8461 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8521 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8581 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8641 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8701 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8761 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8821 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8881 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8941 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9001 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9061 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9121 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9181 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9241 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9301 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9361 cgaaaactct caaggatctt accgctgttg agatccagtt ctatgtaacc cactcgtgca
     9421 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9481 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9541 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9601 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9661 ccacctgacg tcgacggatc gggagatc
//
