LOCUS       sequence-426346-        4761 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A16_STOP
FEATURES             Location/Qualifiers
     source          1..4761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2318..2417)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2434..2453)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2435..2453)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2458..2474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2458..2474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2576..2595)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2587..3396
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2654..2673)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3543..4131
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4032..4051
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4285..4302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4461..4488)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4580..4666)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4715..4737
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4729..4746
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4730..4746
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa gaccgaggcc
      121 cagccttcca catctctgct ggccaacacc agctggaccg gcacagtgat cagcgactcc
      181 gtgccaggct ctcagacatg ggaggataag ggcagcctga ccaggtctgc caccagctgg
      241 acatccgagg cccaggtgtc cgccgcaagg gtggcagagg cacaggcacg cacatctcag
      301 cccaagcaga tcagcgtgct ggaggccctg accgcatccg ccctgaacca gaagcctaca
      361 cacgagaagg tgcagatgac cgagaagaag gagtctgagg tgctgctggc ccggccattc
      421 tggagctcca agaccgagta catcctggcc caagtgggct tctccatgaa gccctcttgc
      481 ctgtggagat ttgcctacct gtggctgaat agcggcggct gttcctttgc cgccatctat
      541 atcttcatgc tgtttctggt gggcgtgcca ctgctgttcc tggagatggc agcaggacag
      601 agcatgaggc agggaggaat gggcgtgtgg aagatcatcg ccccttggat cggcggcgtg
      661 ggctattcta gcttcatggt gtgctttatt ctgggcttgt acttcaatgt agtaaattcc
      721 tggatcatct tttatatgtc tcagagcttc cagtttccag tgccctggga gaagtgcccc
      781 ctgaccatga actcctctgg cttcgacccc gagtgtgaga ggaccacacc ttccatctac
      841 ttttggtatc agcaggccct gaaggcctct gaccgcatcg aggatggcgg ctctccagtg
      901 tacagcctgg tgctgccctt ctttctgtgc tggtgtctgg tgggcgcctt tatgatcaat
      961 ggcctgaaga gcacaggcaa agtgatctac gtgctggtgc tgctgccctg ttttatcatc
     1021 gtgggcttct ttatccggac cctgctgctg gagggagcaa agttcggact gcagcagctg
     1081 gtggtggcca agatctctga cgtgtacaac atgagcgtgt ggagcctggc aggaggacag
     1141 gtgctgagca ataccggaat cggactggga tccgtggcat ctctggccag ctatatgcct
     1201 cagagcaaca attgcctgtc cgatgccttc ctggtgagcg tgatcaacct gctgaccctg
     1261 ctggtgttca catccttcaa tttttgcgtg ctgggctttt gggccaccgt gatcacacac
     1321 cggtgctgtg agagaaacgc cgagatcctg ctgaagctga tcaatctggg caagctgcca
     1381 cctgacgcaa agccacccgt gaacctgctg tacaatccca ccagcatcta taacgcctgg
     1441 ctgtccggcc tgcctcagca catcaagagc atggtgctgc gggaggtgac agagtgcaat
     1501 atcgagaccc agttcctgaa ggcctccgag ggccctaagt tcgcctttct gtccttcgtg
     1561 gaggccatgt cttttctgcc tccatccgtg ttctggtctt ttatcttctt tctgatgctg
     1621 ctggcaatgg gactgagctc cgccatcggc atcatgcagg gcatcatcac cccactgcag
     1681 gataccttca gcttcttcag aaagcacaca aagctgctga tcgtgggcgt gttcctgctg
     1741 atgttcgtgt gcggcctgtt ctttacccgg ccatccggct cttacttcat cagactgctg
     1801 tctgactatt ggatcgtgtt ccccatcatc gtggtggtgg tgtttgagac aatggccgtg
     1861 tcctgggcat acggagcacg gagattcctg gcagatctga ccatcctgct gggccacccc
     1921 atctccccta tctttggctg gctgtggcct cacctgtgcc cagtggtgct gctgatcatc
     1981 ttcgtgacaa tgatggtgca cctgtgcatg aagcctatca catacatgag ctgggattct
     2041 agcacctcca aggaggtgct gaggccatat cccccttggg ccctgctgct gatgatcacc
     2101 ctgttcgcca tcgtgatcct gcctatccca gcctacttcg tgtattgcag gatccaccgc
     2161 atcccatttc gcccaaagag cggcgacgga ccaatgaccg caagcacatc cctgcctctg
     2221 tctcaccagc tgacaccaag caaggaggtg cagaaggagg agatcctgca ggtggatgag
     2281 accaagtacc cctccacctg taatgtgaca tcttaagacc cagctttctt gtacaaagtt
     2341 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     2401 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     2461 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2521 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2581 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     2641 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2701 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2761 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2821 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2881 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2941 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     3001 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     3061 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     3121 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     3181 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     3241 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3301 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3361 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3421 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3481 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3541 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3601 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3661 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3721 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3781 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3841 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3901 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3961 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4021 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4081 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4141 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4201 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4261 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     4321 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4381 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     4441 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     4501 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     4561 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     4621 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     4681 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     4741 gccagtctta agctcgggcc c
//
