LOCUS       sequence-426355-       10047 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Detection of ER-mito contact site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Li X, Gamuyao R, Wu ML, Cho WJ, King SV, Petersen RA, Stabley DR, 
            Lindow C, Climer LK, Shirinifard A, Ferrara F, Throm RE, Robinson 
            CG, Zhou Y, Carisey AF, Tebo AG, Chang CL
  TITLE     A fluorogenic complementation tool kit for interrogating lipid 
            droplet-organelle interaction.
  JOURNAL   J Cell Biol. 2024 Sep 2;223(9):e202311126. doi: 
            10.1083/jcb.202311126. Epub 2024 Jul 1.
  PUBMED    38949658
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2024-09-2"; volume: "223(9):e202311126. doi"; pages: 
            " 10.1083/jcb.202311126. Epub 2024 Jul 1"
COMMENT     Sequence Label: Lenti-FAB(ER-mito)
FEATURES             Location/Qualifiers
     source          1..10047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(864..1655,1656..1724))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1487..1506
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        1863..2242
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2243..2446
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2461..2641
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2688..2813
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3305..3422
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3782..4015
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4200..4244
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        4501..4804
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4805..5008
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5454..5463
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5802..5843
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5889..6179
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     intron          6284..6513
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     6496..6515
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(6585..7159,7160..7162,7163..7171)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6752..6769)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6979..6998
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      7198..7207
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7516..7525
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7522..8412
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     primer_bind     complement(8862..8881)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8927..8982
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8981..9000)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9343..9359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9343..9359)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9356..9378)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9367..9383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9391..9397,9398..9415,9416..9421))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9436..9457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9516..9711
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9562..9697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9717..9851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9754..9773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9808..9827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9919..9936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatggtgg tagaattggt
      721 aaagagagtc gtgtaaaata tcgagttcgc acatcttgtt gtctgattat tgatttttgg
      781 cgaaaccatt tgatcatatg acaagatgtg tatctacctt aacttaatga ttttgataaa
      841 aatcattcta gacgtctcgt gctttaccaa tgcttaatca gtgaggcacc tatctcagcg
      901 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      961 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     1021 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     1081 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1141 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     1201 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1261 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1321 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1381 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1441 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     1501 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1561 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1621 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     1681 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcattgctgg agacgtctag
     1741 aattattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1801 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg
     1861 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     1921 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1981 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2041 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2101 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2161 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2221 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2281 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2341 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2401 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     2461 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2521 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2581 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     2641 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     2701 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     2761 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     2821 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     2881 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaata
     2941 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     3001 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     3061 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     3121 aaaacaaaag taagaaaaaa gcacagcaag cagcaggatc ttcagacctg gaaattccct
     3181 acaatcccca aagtcaagga gtagtagaat ctatgaataa agaattaaag aaaattatag
     3241 gacaggtaag agatcaggct gaacatctta agacagcagt acaaatggca gtattcatcc
     3301 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     3361 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     3421 ttcgggttta ttacagggac agcagaaatc cactttggaa aggaccagca aagctcctct
     3481 ggaaaggtga aggggcagta gtaatacaag ataatagtga cataaaagta gtgccaagaa
     3541 gaaaagcaaa gatcattagg gattatggaa aacagatggc aggtgatgat tgtgtggcaa
     3601 gtagacagga tgaggattag aacatggaaa agtttagtaa aacaccataa ggaggagata
     3661 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     3721 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     3781 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     3841 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     3901 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     3961 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     4021 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     4081 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     4141 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     4201 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     4261 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     4321 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     4381 tatcgtttca gacccacctc ccaaccccga ggggaccgag ctcaagcttc gaacgcgtgc
     4441 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     4501 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     4561 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     4621 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     4681 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     4741 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     4801 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     4861 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     4921 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     4981 acggtgggag gtctatataa gcagagctcg tttagtgaac cgggctcgtt tagtgaaccc
     5041 tcgaggaatt ccgtacggaa ccggtcacca tggagaccgt gagattcggc ggcgacgaca
     5101 tcgagaacag cctggccaag atggacgaca aggccctgga caagctggcc ttcggcgcca
     5161 tccagctgga cggcaacggc aagatcatcc actacaacgc cgccgagggc accatcaccg
     5221 gcagagaccc caagaccgtg atcggcaaga acttcttcac cgacgtggcc cccggcaccc
     5281 agagcaagga gttccagggc agattcaagg agggcgtgca gaagggcgac ctgaacacca
     5341 tgttcgagtg gatgatcccc accagcagag gccccaccaa ggtgaaggtg cacatgaaga
     5401 aggccatgac ctccggagga ggcggcagcg gcggaggggg atctctaccg gtcgccacca
     5461 tggtgagcaa gggcgaggag ggcagcggcg gagggggatc aggagggggc gagatgtggc
     5521 atgaaggcct ggaagaggca tctcgtttgt actttgggga aaggaacgtg aaaggcatgt
     5581 ttgaggtgct ggagcccttg catgctatga tggaacgggg cccccagact ctgaaggaaa
     5641 catcctttaa tcaggcctat ggtcgagatt taatggaggc ccaagagtgg tgcaggaagt
     5701 acatgaaatc agggaatgtc aaggacctca cccaagcctg ggacctctat tatcatgtgt
     5761 tccgacgaat ctcaagtggc agcggaggta gtgggagcgg tggcaagccc atccccaacc
     5821 ccctgctggg cctggacagc accaaactta atggcatgga cgagctgtac aagtccggac
     5881 tcagatctat gcctggtccg acccccagtg gcactaacgt gggatcctca gggcgctctc
     5941 ccagcaaagc agtggccgcc cgggcggcgg gatccactgt ccggcagagg aaaaatgcca
     6001 gctgtgggac aaggagtgca ggccgcacaa cctcggcagg caccgggggg atgtggcgat
     6061 tctacacaga agattcacct gggctcaaag ttggccctgt tccagtattg gttatgagtc
     6121 ttctgttcat cgcttctgta tttatgttgc acatttgggg caagtacact cgttcgtagg
     6181 aattctgcag tcgacggtac atggccgagg actaagcggc cgcatagata actgatccag
     6241 tgtgctggaa ttaattcgct gtctgcgagg gccagctgtt ggggtgagta ctccctctca
     6301 aaagcgggca tgacttctgc gctaagattg tcagtttcca aaaacgagga ggatttgata
     6361 ttcacctggc ccgcggtgat gcctttgagg gtggccgcgt ccatctggtc agaaaagaca
     6421 atctttttgt tgtcaagctt gaggtgtggc aggcttgaga tctggccata cacttgagtg
     6481 acaatgacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcagg
     6541 tcgagcatgc atctagggcg gccaattccg cccctctccc cccccccctc tccctccccc
     6601 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     6661 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     6721 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     6781 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     6841 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     6901 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     6961 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     7021 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     7081 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     7141 tgaaaaacac gatgataagc ttgccacaac ccgggaggcg cgccgtcaga tccgctagcc
     7201 accatgggtg acagctattg ggtctttgtg aaacggacta gtgaaaccga aaccggaggc
     7261 ggctccggag gcagcggcgg ttctggaggt tccaccctac cggtctcagc tggaggcagt
     7321 gctggtggaa gtgccggagg tagaattcta gaaggtgccg caagagaggc tgccgctagg
     7381 gaagcagctg cacgtgaggc agctgctaga gaagctgcag ccagggaggc tgcagctaga
     7441 gcatcaggtg gtggcggttc aggcggaggt ggatctggag gtggcggatc gggtggggga
     7501 ggttcgacac cggtcgccac catggcagaa atcggtactg gctttccatt cgacccccat
     7561 tatgtggaag tcctgggcga gcgcatgcac tacgtcgatg ttggtccgcg cgatggcacc
     7621 cctgtgctgt tcctgcacgg taacccgacc tcctcctacg tgtggcgcaa catcatcccg
     7681 catgttgcac cgacccatcg ctgcattgct ccagacctga tcggtatggg caaatccgac
     7741 aaaccagacc tgggttattt cttcgacgac cacgtccgct tcatggatgc cttcatcgaa
     7801 gccctgggtc tggaagaggt cgtcctggtc attcacgact ggggctccgc tctgggtttc
     7861 cactgggcca agcgcaatcc agagcgcgtc aaaggtattg catttatgga gttcatccgc
     7921 cctatcccga cctgggacga atggccagaa tttgcccgcg agaccttcca ggccttccgc
     7981 accaccgacg tcggccgcaa gctgatcatc gatcagaacg tttttatcga gggtacgctg
     8041 ccgatgggtg tcgtccgccc gctgactgaa gtcgagatgg accattaccg cgagccgttc
     8101 ctgaatcctg ttgaccgcga gccactgtgg cgcttcccaa acgagctgcc aatcgccggt
     8161 gagccagcga acatcgtcgc gctggtcgaa gaatacatgg actggctgca ccagtcccct
     8221 gtcccgaagc tgctgttctg gggcacccca ggcgttctga tcccaccggc cgaagccgct
     8281 cgcctggcca aaagcctgcc taactgcaag gctgtggaca tcggcccggg tctgaatctg
     8341 ctgcaagaag acaacccgga cctgatcggc agcgagatcg cgcgctggct gtcgacgctc
     8401 gagatttccg gcatgtacaa gtccggactc agatctcgag ctcaagcttc gaattctttg
     8461 caggtgcaga atggacctat aggacatcga ggtgaagggg acccaagtgg tattcccata
     8521 tttatggtgc tggtgccagt gtttgccctc accatggtag cagcctgggc tttcatgaga
     8581 taccggcaac aactttgata aggatccacc ggatcggtac aaggcggccg cactaggtac
     8641 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     8701 ggggactgga agggctaatt cactcccaaa gaagacaaga tctgcttttt gcctgtactg
     8761 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8821 tgcttaagcc tcaataaagc ttgccttgtc gtgctagcag atctttttcc ctctgccaaa
     8881 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     8941 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     9001 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     9061 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     9121 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     9181 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     9241 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     9301 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     9361 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     9421 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     9481 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
     9541 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9601 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9661 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
     9721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9841 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9901 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10021 aggaaagaac atgtgagcaa aaggcca
//
