LOCUS       sequence-426583-       12743 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Destination vector for cloning BioID2 fusion proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12743)
  TITLE     Systematic Identification of Post-Transcriptional Regulatory Modules
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pWPI_GW_BioID2_T2A_Blast
FEATURES             Location/Qualifiers
     source          1..12743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3529..3653
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3678..3683,3684..3701,3702..3708)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3762..4421
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3828..3847)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4763..5068
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4947..4966
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5109..5233)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5283..5978
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     CDS             5979..6005
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6015..6068
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6081..6473
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(6544..6560)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6545..6561)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6574..7162
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6627..6647)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7045..7056)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7306..7339)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7359..7539
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7564..7582)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7565..7582)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7872..7891)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8105..8124)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8224..8246
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8284..8302)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8370..8474
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8475..8543,8544..9335)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8693..8712)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9506..10094
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9995..10014
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10248..10265
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10335..10355)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10340..10669
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10520..10655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11803..11868
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11998..12018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12443..12577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12480..12499)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12534..12553
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttaaac ggtacccaac aagtttgtac
     3541 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     3601 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
     3661 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     3721 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     3781 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     3841 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     3901 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     3961 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     4021 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     4081 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     4141 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     4201 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     4261 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     4321 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     4381 aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac
     4441 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     4501 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     4561 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     4621 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     4681 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     4741 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     4801 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     4861 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     4921 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     4981 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     5041 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     5101 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     5161 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     5221 tgtacaaagt ggtttatggt ggaggcgggt ctggaggcgg gggtagtggc gggggtggaa
     5281 gcttcaagaa cctgatctgg ctgaaggagg tggacagcac ccaggagaga ctgaaggagt
     5341 ggaacgtgag ctacggcacc gccctggtgg ccgacagaca gaccaagggc agaggcggcc
     5401 tgggcagaaa gtggctgagc caggagggcg gcctgtactt cagcttcctg ctgaacccca
     5461 aggagttcga gaacctgctg cagctgcccc tggtgctggg cctgagcgtg agcgaggccc
     5521 tggaggagat caccgagatc cccttcagcc tgaagtggcc caacgacgtg tacttccagg
     5581 agaagaaggt gagcggcgtg ctgtgcgagc tgagcaagga caagctgatc gtgggcatcg
     5641 gcatcaacgt gaaccagaga gagatccccg aggagatcaa ggacagagcc accaccctgt
     5701 acgagatcac cggcaaggac tgggacagaa aggaggtgct gctgaaggtg ctgaagagaa
     5761 tcagcgagaa cctgaagaag ttcaaggaga agagcttcaa ggagttcaag ggcaagatcg
     5821 agagcaagat gctgtacctg ggcgaggagg tgaagctgct gggcgagggc aagatcaccg
     5881 gcaagctggt gggcctgagc gagaagggcg gcgccctgat cctgaccgag gagggcatca
     5941 aggagatcct gagcggcgag ttcagcctga gaagaagcta cccttacgat gtaccggatt
     6001 acgcaggatc gggagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc
     6061 ctggcccacc ggtcgccacc gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     6121 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     6181 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     6241 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     6301 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     6361 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     6421 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggcctgcagc
     6481 tgcagtagta agaattcgct agctagtcga gctcaagctt cgaattcgat atcaagctta
     6541 tcgcgatacc gtcgacctcg agggaattcc gataatcaac ctctggatta caaaatttgt
     6601 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6661 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6721 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6781 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6841 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6901 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6961 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     7021 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7081 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7141 tccctttggg ccgcctcccc gcatcgggaa ttcgagctcg gtacctttaa gaccaatgac
     7201 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7261 aattcactcc caacgaagac aagatgggat caattcacca tgggaataac ttcgtatagc
     7321 atacattata cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7381 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7441 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7501 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     7561 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     7621 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     7681 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     7741 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     7801 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     7861 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     7921 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     7981 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     8041 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
     8101 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     8161 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     8221 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     8281 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     8341 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8401 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8461 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8521 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8581 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8641 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8701 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8761 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8821 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8881 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8941 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9001 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     9061 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9121 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9181 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9241 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9301 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9361 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9421 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9481 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9541 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9601 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9661 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9721 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9781 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9841 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9901 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9961 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10021 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10081 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10141 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10201 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10261 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10321 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
    10381 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    10441 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    10501 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    10561 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10621 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
    10681 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
    10741 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
    10801 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
    10861 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
    10921 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
    10981 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
    11041 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
    11101 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
    11161 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
    11221 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
    11281 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
    11341 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
    11401 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
    11461 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
    11521 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
    11581 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
    11641 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
    11701 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
    11761 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
    11821 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
    11881 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
    11941 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
    12001 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
    12061 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
    12121 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    12181 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    12241 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    12301 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    12361 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    12421 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    12481 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    12541 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    12601 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    12661 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    12721 aatatgtact acaaacttag tag
//
