LOCUS       sequence-426777-        8462 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express GRAB-PGD2-1.0 sensor and membrane tethered mCherry under CMV
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Sang D, Lin K, Yang Y, Ran G, Li B, Chen C, Li Q, Ma Y, Lu L, Cui 
            XY, Liu Z, Lv SQ, Luo M, Liu Q, Li Y, Zhang EE
  TITLE     Prolonged sleep deprivation induces a cytokine-storm-like syndrome 
            in mammals.
  JOURNAL   Cell. 2023 Dec 7;186(25):5500-5516.e21. doi: 
            10.1016/j.cell.2023.10.025. Epub 2023 Nov 27.
  PUBMED    38016470
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.10.025"; journalName: "Cell"; date: "2023-12-7-
            7"; volume: "186"; issue: "25"; pages: "5500-5516.e21"
COMMENT     Sequence Label: pDisplay-PGD2-1.0-IRES-mcherry
FEATURES             Location/Qualifiers
     source          1..8462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      719..728
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     725..787
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     protein_bind    788..812
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1804..1825
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1931..1952)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2192..2211)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    join(2661..3234,3235..3237,3238..3246)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2828..2845)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3054..3073
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3247..3954
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3392..3410)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3660..3679
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4012..4041
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4045..4191
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4213..4230)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4219..4443
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4575..5163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4655..4674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5492..5539
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5564..5583
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5771..6565)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5882..5901)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6492..6511
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6664..6683)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6762..6953
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6910..6930
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7013..7804,7805..7873))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7636..7655
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7874..7978)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8005..8460
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8092..8111)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8302..8323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc
      721 caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac
      781 tggtgacaca agtttgtaca aaaaagttgg caccaccggt aagcagatgt tctaccgctg
      841 ccaaaacacc acctcggtgg agaaagatca ctcggcgacg atgggctggg tgctcttcag
      901 cgccggcctc ctgggcaacc tgctggcgct ggggctgctg gcgcgctcgg ggctggggtc
      961 gtgcccgctg cgctcgccac gccaggcgcc ttcggtcttc tgtgtgcttg tgtgtggcct
     1021 gaccatcacc gacttgcttg gcaagtgcct cttgagcccc gtcgtgctag cagcctacgc
     1081 gcagaaccgg agcctgtggg gactggtgtc cacgccgggc agctctctgt gccaagcctt
     1141 cgcctttagt atgtctttct tcggcctcgc ctcgacgctg cagctcctgg ccatggccct
     1201 ggagtgttgg ctatccctgg gacacccctt cttctaccga cagtacatca ccctccgccg
     1261 gggcgtgctg gtggcgcccg tcgtgagcac cttctccttg gctttctgcg cgctgccctt
     1321 cgcaggcttc ggaaagttcg tgcagtactg ccccggcact tggtgcttca tccagatggc
     1381 ccacgaggag cgctcgccgt cggtgctggg ctactcagtg ctgtatgcca gcatcctggc
     1441 gctgctggtc ctcgccattg tgctgtgcaa cctgagcgcc atgcgcaagc tctaccgcat
     1501 gcaccgccga tatcggagac agctcaacgg ctggcctggc gagcccgcgc cggccgggcc
     1561 gcgcgacacc gacgcgctgg acctggagga gggaggaaac gtctatatca aggccgacaa
     1621 gcagaagaac ggcatcaagg cgaacttcca gatccgccac aacatcgagg acggcggcgt
     1681 gcagctcgcc taccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1741 cgacaaccac tacctgagcg tgcagtccaa actttcgaaa gaccccaacg agaagcgcga
     1801 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actgccggca tggacgagct
     1861 gtacaagggc ggtaccggag ggagcatggt gagaaagggc gaggagctgt tcaccggggt
     1921 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1981 cgagggtgag ggcgatgcca ccgagggcaa gctgaccctg aagttcatct gcaccaccgg
     2041 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     2101 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2161 ctacatccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2221 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2281 ggaggacggc aacatcctgg ggcacaagct ggagtacaac accggagcag cagcacgcat
     2341 ggcggagcac attgaccacc tcattctcct ggctatcatg accgtgctct tcgtgatgtg
     2401 ctccctgcct ttaacttatc gtgcttacta tggagcattt aaagctgtca cccaggatgg
     2461 aacctctgaa gaagtggaag accttcgcgc cttgcgtttt atatctgtga tctcaattgt
     2521 ggacccttgg attttcatca ttttcagaac ctctgttttt cggatgtttt tccacaagat
     2581 tttcatcagg cctctgaggt atagaaattg gcatagcaat ccctgccaaa atgacatgga
     2641 atccagcctg taagctagcg cccctctccc tccccccccc ctaacgttac tggccgaagc
     2701 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     2761 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     2821 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     2881 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     2941 ccacctggcg acaggtgcct ctgcggccaa cgccacgtgt ataagataca cctgcaaagg
     3001 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     3061 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     3121 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     3181 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     3241 acaaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     3301 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     3361 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     3421 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     3481 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     3541 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     3601 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     3661 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     3721 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     3781 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     3841 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     3901 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caaggatggt
     3961 aaaaagaaga aaaagaagtc aaagacaaag tgtgtaatta tgtaagtcga cgaacaaaaa
     4021 ctcatctcag aagaggatct gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg
     4081 ccacactcct tgccctttaa ggtggtggtg atctcagcca tcctggccct ggtggtgctc
     4141 accatcatct cccttatcat cctcatcatg ctttggcaga agaagccacg ttaggcggcc
     4201 gctcgagatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4261 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4321 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4381 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4441 tggcttctga ggcggaaaga accagtggcg gtaatacggt tatccacaga atcgggggat
     4501 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4561 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4621 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4681 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4741 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4801 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4861 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacgcgactt atcgccactg
     4921 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4981 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5041 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5101 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5161 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5221 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5281 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat ggcagggcct
     5341 gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg gtggggtggg
     5401 gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt atcgacagag
     5461 tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc gtgcgtttta
     5521 ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt
     5581 tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc gcgctggagg
     5641 atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg
     5701 gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc
     5761 agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     5821 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     5881 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     5941 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     6001 cataggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt
     6061 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     6121 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     6181 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     6241 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     6301 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     6361 acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga
     6421 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     6481 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     6541 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcgatct
     6601 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     6661 gggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
     6721 aactgggcgg agttaggggc ggaatgggcg gagttagggg cgggactatg gttgctgact
     6781 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
     6841 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     6901 actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt
     6961 cctttttcaa taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7021 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7081 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7141 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7201 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7261 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7321 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7381 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7441 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7501 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7561 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7621 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7681 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7741 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7801 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7861 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7921 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7981 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8041 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8101 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8161 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8221 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8281 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     8341 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     8401 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     8461 ac
//
