LOCUS       sequence-426789-       10389 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses AcGFP-MSH2 in mammalian cells from a retroviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 10389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: AcGFP-MSH2
FEATURES             Location/Qualifiers
     source          1..10389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2910
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        5784..6283
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5819..5842)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6216..6235
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6304..6903
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6304..6323)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6800..6820
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7088..7520
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7623..7645)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7781..7801)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7786..8115
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7966..8101
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8137..8156
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8237..8254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8408..8996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8488..8507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9167..9958,9959..10027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9790..9809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10028..10132)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10200..10218
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10318..10389,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcgc cgagctgttc
     2221 accggcatcg tgcccatcct gatcgagctg aatggcgatg tgaatggcca caagttcagc
     2281 gtgagcggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2341 accaccggca agctgcctgt gccctggccc accctggtga ccaccctgag ctacggcgtg
     2401 cagtgcttct cacgctaccc cgatcacatg aagcagcacg acttcttcaa gagcgccatg
     2461 cctgagggct acatccagga gcgcaccatc ttcttcgagg atgacggcaa ctacaagtcg
     2521 cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc gcatcgagct gaccggcacc
     2581 gatttcaagg aggatggcaa catcctgggc aataagatgg agtacaacta caacgcccac
     2641 aatgtgtaca tcatgaccga caaggccaag aatggcatca aggtgaactt caagatccgc
     2701 cacaacatcg aggatggcag cgtgcagctg gccgaccact accagcagaa tacccccatc
     2761 ggcgatggcc ctgtgctgct gcccgataac cactacctgt ccacccagag cgccctgtcc
     2821 aaggacccca acgagaagcg cgatcacatg atctacttcg gcttcgtgac cgccgccgcc
     2881 atcacccacg gcatggatga gctgtacaag tccggactca gatctcgagg aggcggagga
     2941 agcgcggtgc agccgaagga gacgctgcag ttggagagcg cggccgaggt cggcttcgtg
     3001 cgcttctttc agggcatgcc ggagaagccg accaccacag tgcgcctttt cgaccggggc
     3061 gacttctata cggcgcacgg cgaggacgcg ctgctggccg cccgggaggt gttcaagacc
     3121 cagggggtga tcaagtacat ggggccggca ggagcaaaga atctgcagag tgttgtgctt
     3181 agtaaaatga attttgaatc ttttgtaaaa gatcttcttc tggttcgtca gtatagagtt
     3241 gaagtttata agaatagagc tggaaataag gcatccaagg agaatgattg gtatttggca
     3301 tataaggctt ctcctggcaa tctctctcag tttgaagaca ttctctttgg taacaatgat
     3361 atgtcagctt ccattggtgt tgtgggtgtt aaaatgtccg cagttgatgg ccagagacag
     3421 gttggagttg ggtatgtgga ttccatacag aggaaactag gactgtgtga attccctgat
     3481 aatgatcagt tctccaatct tgaggctctc ctcatccaga ttggaccaaa ggaatgtgtt
     3541 ttacccggag gagagactgc tggagacatg gggaaactga gacagataat tcaaagagga
     3601 ggaattctga tcacagaaag aaaaaaagct gacttttcca caaaagacat ttatcaggac
     3661 ctcaaccggt tgttgaaagg caaaaaggga gagcagatga atagtgctgt attgccagaa
     3721 atggagaatc aggttgcagt ttcatcactg tctgcggtaa tcaagttttt agaactctta
     3781 tcagatgatt ccaactttgg acagtttgaa ctgactactt ttgacttcag ccagtatatg
     3841 aaattggata ttgcagcagt cagagccctt aacctttttc agggttctgt tgaagatacc
     3901 actggctctc agtctctggc tgccttgctg aataagtgta aaacccctca aggacaaaga
     3961 cttgttaacc agtggattaa gcagcctctc atggataaga acagaataga ggagagattg
     4021 aatttagtgg aagcttttgt agaagatgca gaattgaggc agactttaca agaagattta
     4081 cttcgtcgat tcccagatct taaccgactt gccaagaagt ttcaaagaca agcagcaaac
     4141 ttacaagatt gttaccgact ctatcagggt ataaatcaac tacctaatgt tatacaggct
     4201 ctggaaaaac atgaaggaaa acaccagaaa ttattgttgg cagtttttgt gactcctctt
     4261 actgatcttc gttctgactt ctccaagttt caggaaatga tagaaacaac tttagatatg
     4321 gatcaggtgg aaaaccatga attccttgta aaaccttcat ttgatcctaa tctcagtgaa
     4381 ttaagagaaa taatgaatga cttggaaaag aagatgcagt caacattaat aagtgcagcc
     4441 agagatcttg gcttggaccc tggcaaacag attaaactgg attccagtgc acagtttgga
     4501 tattactttc gtgtaacctg taaggaagaa aaagtccttc gtaacaataa aaactttagt
     4561 actgtagata tccagaagaa tggtgttaaa tttaccaaca gcaaattgac ttctttaaat
     4621 gaagagtata ccaaaaataa aacagaatat gaagaagccc aggatgccat tgttaaagaa
     4681 attgtcaata tttcttcagg ctatgtagaa ccaatgcaga cactcaatga tgtgttagct
     4741 cagctagatg ctgttgtcag ctttgctcac gtgtcaaatg gagcacctgt tccatatgta
     4801 cgaccagcca ttttggagaa aggacaagga agaattatat taaaagcatc caggcatgct
     4861 tgtgttgaag ttcaagatga aattgcattt attcctaatg acgtatactt tgaaaaagat
     4921 aaacagatgt tccacatcat tactggcccc aatatgggag gtaaatcaac atatattcga
     4981 caaactgggg tgatagtact catggcccaa attgggtgtt ttgtgccatg tgagtcagca
     5041 gaagtgtcca ttgtggactg catcttagcc cgagtagggg ctggtgacag tcaattgaaa
     5101 ggagtctcca cgttcatggc tgaaatgttg gaaactgctt ctatcctcag gtctgcaacc
     5161 aaagattcat taataatcat agatgaattg ggaagaggaa cttctaccta cgatggattt
     5221 gggttagcat gggctatatc agaatacatt gcaacaaaga ttggtgcttt ttgcatgttt
     5281 gcaacccatt ttcatgaact tactgccttg gccaatcaga taccaactgt taataatcta
     5341 catgtcacag cactcaccac tgaagagacc ttaactatgc tttatcaggt gaagaaaggt
     5401 gtctgtgatc aaagttttgg gattcatgtt gcagagcttg ctaatttccc taagcatgta
     5461 atagagtgtg ctaaacagaa agccctggaa cttgaggagt ttcagtatat tggagaatcg
     5521 caaggatatg atatcatgga accagcagca aagaagtgct atctggaaag agagcaaggt
     5581 gaaaaaatta ttcaggagtt cctgtccaag gtgaaacaaa tgccctttac tgaaatgtca
     5641 gaagaaaaca tcacaataaa gttaaaacag ctaaaagctg aagtaatagc aaagaataat
     5701 agctttgtaa atgaaatcat ttcacgaata aaagttacta cgtgaggatc caccggatct
     5761 agataactga tcataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     5821 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     5881 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     5941 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     6001 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     6061 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     6121 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     6181 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     6241 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
     6301 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6361 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6421 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6481 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6541 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6601 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6661 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6721 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6781 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6841 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6901 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg
     6961 gcgtctcgat cgagatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc
     7021 tgaagcctat agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa
     7081 aggggggaat gaaagacccc acctgtaggt ttggcaagct actagcttaa gtaacgccat
     7141 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     7201 cagatggaac agggtcgatc gaccctagag aaccatcaga tgtttccagg gtgccccaag
     7261 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     7321 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     7381 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     7441 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     7501 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     7561 caccgaccca ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa
     7621 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7681 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     7741 acccagtcac gtagcgatag cggagtgtag atccggctgt ggaatgtgtg tcagttaggg
     7801 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7861 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7921 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7981 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8041 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8101 ctaggctttt gcaaaaagct tactggctta actatgcggc atcagagcag attgtactga
     8161 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     8221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8821 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9481 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9781 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10141 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    10201 ataggcgtat cacgaggccc tttcgtcttc aagaattagc ttggccattg catacgttgt
    10261 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac
    10321 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
    10381 atatggagt
//
