LOCUS       sequence-427357-       12838 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encoding a Forster resonance energy transfer (FRET) biosensor for 
            Rab5 activity with cyan and yellow fluorescent protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12838)
  TITLE     Collective cell migration is coordinated by front-biased activation 
            of the feed-forward loop consisting of Ras, Rab5, and Rac1.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCSIIbsr-Raichu-Rab5/PM
FEATURES             Location/Qualifiers
     source          1..12838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2585..3747
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2816..3738
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3695..3715
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3782..4492
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELY"
     CDS             join(5078..5080,5081..5083,5084..5596)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(5123..5144)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5384..5403)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5731..5752
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(6504..7078,7079..7081,7082..7090)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6671..6688)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6898..6917
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7091..7489
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7533..8121
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7586..7606)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8004..8015)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8640..8820
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8846..8863)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8852..9076
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9122..9550
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9209..9228)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9419..9440
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9559..9579)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9564..9893
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9744..9879
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9806..9825
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9941..9988
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10007..10381
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10511..10632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10548..10567)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10602..10621
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10681..10697)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10681..10697)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10694..10716)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10705..10721
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10729..10735,10736..10753,10754..10759))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10774..10795
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10912..10929)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11083..11671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11163..11182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11842..12633,12634..12702))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12465..12484
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12703..12807)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     2701 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2761 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2821 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2881 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
     2941 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     3001 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3061 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3121 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3181 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3241 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3301 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3361 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
     3421 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
     3481 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
     3541 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3601 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3661 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3721 ttttttcttc catttcaggt gtcgtgaaca cgctaccggt ctcgacgaat tcgcccttag
     3781 catgtctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg
     3841 tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacgtacgg
     3901 taaattgacc ttaaaattac tctgtactac tggtaaattg ccagttccat ggccaacctt
     3961 agtcactact ttaggttatg gtgttcaatg ttttgctaga tacccagatc atatgaaaca
     4021 acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt
     4081 caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt
     4141 taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa
     4201 attggaatac aactataact ctcacaatgt ttacatcact gctgacaaac aaaagaatgg
     4261 tatcaaagct aacttcaaaa ttagacacaa cattgaagat ggtggtgttc aattagctga
     4321 ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta
     4381 cttatcctat caatctgcct tattcaaaga tccaaacgaa aagagagacc acatggtctt
     4441 gttagaattt ttgactgctg ctggtattac cgagggtatg aatgaattgt acctcgagag
     4501 ctcttcagaa ggtttcatat gtccccagtg tatgaaatct cttggatctg ctgatgaact
     4561 tttcaaacat tatgaagctg ttcatgatgc tggtaatgac tcaggtcacg gaggagagtc
     4621 taatcttgct ttgaagcgag atgatgtaac actgctcaga caagaggtcc aagacctaca
     4681 ggcttcactt aaggaagaaa aatggtactc ggaagaatta aagaaggaat tagaaaaata
     4741 tcaagggctg cagcagcaag aggccaaacc tgatgggttg gtgactgatt catcagcaga
     4801 actacagtct ttggaacagc aattagaaga agcccaaaca gaaaatttta atattaagca
     4861 aatgaaagac ttatttgaac agaaagcagc ccaacttgct actgaaattg cagatataaa
     4921 gtcaaagtat gatgaagaaa ggagtcttcg agaagctgct gaacaaaaag tgacacgtct
     4981 gacagaagaa ttaaacaaag aggcaactgt aattcaagat ctgaagacgg aactgcttca
     5041 gagaccttcc ggaggtggaa ccggtggtgg aggtaccatg gtgagcaagg gcgaggagct
     5101 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaggtt
     5161 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     5221 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg
     5281 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     5341 catgcccgaa ggctacgtcc aggagcgtac catcttcttc aaggacgacg gcaactacaa
     5401 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     5461 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag
     5521 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggccc acttcaagat
     5581 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     5641 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     5701 tagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     5761 cgggatcact ctcggcatgg acgagctgtg cggccgcatg gctaatcgag gagcaacaag
     5821 acccaacggg ccaaatactg gaaataaaat atgccagttc aaactagtac ttctgggaga
     5881 gtctgctgtt ggcaaatcaa gcctagtgct tcgttttgtg aagggccaat ttcatgaatt
     5941 tcaagagagt accatagggg ctgcttttct aacccaaact gtgtgtcttg atgatacaac
     6001 agtaaagttt gaaatatggg atacagctgg tcaagaacga taccatagct tagcaccaat
     6061 gtactacaga ggagcacaag cagccatagt tgtatatgat atcacaaatg aggagtcctt
     6121 tgccagagcc aaaaactggg ttaaagaact tcagaggcaa gccagtccta acattgtaat
     6181 agctttatca ggaaacaagg ctgatcttgc aaataaaaga gctgtcgatt tccaggaagc
     6241 acagtcctat gcagatgaca acagtttatt attcatggag acatcagcta aaacatcgat
     6301 gaatgtaaat gaaatattca tggcaatagc taaaaagttg ccaaagaacg aaccacagaa
     6361 tccaggagca aattctgcca gaggaagagg agtagacctt actgaaccca cgcagccaac
     6421 caggagttct agaaagatga gcaaagatgg taaaaagaag aaaaagaagt caaagacaaa
     6481 gtgtgtaatt atgtaaggat ccgcccctct ccctcccccc cccctaacgt tactggccga
     6541 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     6601 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     6661 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     6721 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     6781 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     6841 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     6901 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     6961 ggatctgatc tggggcctcg gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa
     7021 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
     7081 ggccacaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     7141 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     7201 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     7261 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     7321 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     7381 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     7441 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag tcgagcctct
     7501 agagttaaca tcgagggatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     7561 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7621 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7681 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7741 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7801 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7861 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7921 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7981 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8041 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8101 ccctttgggc cgcctccccg catcgatacc gtcgagacct agaaaaacat ggagcaatca
     8161 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     8221 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     8281 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     8341 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     8401 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     8461 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     8521 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     8581 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     8641 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8701 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8761 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8821 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     8881 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     8941 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     9001 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     9061 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     9121 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9181 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9241 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9301 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9361 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9421 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9481 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9541 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     9601 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     9661 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     9721 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     9781 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     9841 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     9901 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     9961 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
    10021 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
    10081 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
    10141 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
    10201 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
    10261 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
    10321 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
    10381 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    10441 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    10501 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10621 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
    10681 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10741 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    10801 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10861 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    10921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    11041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    11101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    11161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    11221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    11281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    11341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    11521 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    11581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    11701 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    11761 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    11821 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    11881 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11941 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    12001 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    12061 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    12121 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    12181 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    12241 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    12301 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    12361 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    12421 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    12481 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    12541 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    12601 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    12661 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    12721 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    12781 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
