LOCUS       sequence-427415-       13332 bp    DNA     circular SYN 14-APR-2025
DEFINITION  2nd generation lentiviral vector for reversible immortalization of 
            human primary cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13332)
  AUTHORS   Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D
  TITLE     Reversible immortalization of human primary cells by 
            lentivector-mediated transfer of specific genes.
  JOURNAL   Mol Ther. 2000 Oct . 2(4):404-14.
  PUBMED    11020357
REFERENCE   2  (bases 1 to 13332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Oct . 2(4):404-14."
COMMENT     Sequence Label: pLOX-TERT-iresTK
FEATURES             Location/Qualifiers
     source          1..13332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             92..725
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    772..897
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1390..1623
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1808..1852
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2131..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2635..2659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2782..6180
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     misc_feature    6233..6694
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6289..6306)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6516..6535
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6699..7829
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     protein_bind    complement(7985..8018)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8038..8218
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8243..8261)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8244..8261)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8551..8570)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8784..8803)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8903..8925
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8963..8981)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9049..9153
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9154..9222,9223..10014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9372..9391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10185..10773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10674..10693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10927..10944
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11014..11034)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11019..11348
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11199..11334
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12482..12547
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12677..12697
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13122..13256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13159..13178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13213..13232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
       61 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
      121 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
      181 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
      241 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
      301 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
      361 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
      421 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
      481 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      541 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      601 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      661 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      721 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      781 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      841 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      901 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      961 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     1021 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1081 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1141 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1201 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     1261 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1321 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1381 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1441 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1501 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1561 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1621 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1681 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1741 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1801 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1861 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1921 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1981 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     2041 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2101 tcgacggtat cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2401 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2581 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     2641 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     2701 acaccgactc tagctagagg atctactagt gaattcgtcc tgctgcgcac gtgggaagcc
     2761 ctggccccgg ccacccccgc gatgccgcgc gctccccgct gccgagccgt gcgctccctg
     2821 ctgcgcagcc actaccgcga ggtgctgccg ctggccacgt tcgtgcggcg cctggggccc
     2881 cagggctggc ggctggtgca gcgcggggac ccggcggctt tccgcgcgct ggtggcccag
     2941 tgcctggtgt gcgtgccctg ggacgcacgg ccgccccccg ccgccccctc cttccgccag
     3001 gtgtcctgcc tgaaggagct ggtggcccga gtgctgcaga ggctgtgcga gcgcggcgcg
     3061 aagaacgtgc tggccttcgg cttcgcgctg ctggacgggg cccgcggggg cccccccgag
     3121 gccttcacca ccagcgtgcg cagctacctg cccaacacgg tgaccgacgc actgcggggg
     3181 agcggggcgt gggggctgct gctgcgccgc gtgggcgacg acgtgctggt tcacctgctg
     3241 gcacgctgcg cgctctttgt gctggtggct cccagctgcg cctaccaggt gtgcgggccg
     3301 ccgctgtacc agctcggcgc tgccactcag gcccggcccc cgccacacgc tagtggaccc
     3361 cgaaggcgtc tgggatgcga acgggcctgg aaccatagcg tcagggaggc cggggtcccc
     3421 ctgggcctgc cagccccggg tgcgaggagg cgcgggggca gtgccagccg aagtctgccg
     3481 ttgcccaaga ggcccaggcg tggcgctgcc cctgagccgg agcggacgcc cgttgggcag
     3541 gggtcctggg cccacccggg caggacgcgt ggaccgagtg accgtggttt ctgtgtggtg
     3601 tcacctgcca gacccgccga agaagccacc tctttggagg gtgcgctctc tggcacgcgc
     3661 cactcccacc catccgtggg ccgccagcac cacgcgggcc ccccatccac atcgcggcca
     3721 ccacgtccct gggacacgcc ttgtcccccg gtgtacgccg agaccaagca cttcctctac
     3781 tcctcaggcg acaaggagca gctgcggccc tccttcctac tcagctctct gaggcccagc
     3841 ctgactggcg ctcggaggct cgtggagacc atctttctgg gttccaggcc ctggatgcca
     3901 gggactcccc gcaggttgcc ccgcctgccc cagcgctact ggcaaatgcg gcccctgttt
     3961 ctggagctgc ttgggaacca cgcgcagtgc ccctacgggg tgctcctcaa gacgcactgc
     4021 ccgctgcgag ctgcggtcac cccagcagcc ggtgtctgtg cccgggagaa gccccagggc
     4081 tctgtggcgg cccccgagga ggaggacaca gacccccgtc gcctggtgca gctgctccgc
     4141 cagcacagca gcccctggca ggtgtacggc ttcgtgcggg cctgcctgcg ccggctggtg
     4201 cccccaggcc tctggggctc caggcacaac gaacgccgct tcctcaggaa caccaagaag
     4261 ttcatctccc tggggaagca tgccaagctc tcgctgcagg agctgacgtg gaagatgagc
     4321 gtgcggggct gcgcttggct gcgcaggagc ccaggggttg gctgtgttcc ggccgcagag
     4381 caccgtctgc gtgaggagat cctggccaag ttcctgcact ggctgatgag tgtgtacgtc
     4441 gtcgagctgc tcaggtcttt cttttatgtc acggagacca cgtttcaaaa gaacaggctc
     4501 tttttctacc ggaagagtgt ctggagcaag ttgcaaagca ttggaatcag acagcacttg
     4561 aagagggtgc agctgcggga gctgtcggaa gcagaggtca ggcagcatcg ggaagccagg
     4621 cccgccctgc tgacgtccag actccgcttc atccccaagc ctgacgggct gcggccgatt
     4681 gtgaacatgg actacgtcgt gggagccaga acgttccgca gagaaaagag ggccgagcgt
     4741 ctcacctcga gggtgaaggc actgttcagc gtgctcaact acgagcgggc gcggcgcccc
     4801 ggcctcctgg gcgcctctgt gctgggcctg gacgatatcc acagggcctg gcgcaccttc
     4861 gtgctgcgtg tgcgggccca ggacccgccg cctgagctgt actttgtcaa ggtggatgtg
     4921 acgggcgcgt acgacaccat cccccaggac aggctcacgg aggtcatcgc cagcatcatc
     4981 aaaccccaga acacgtactg cgtgcgtcgg tatgccgtgg tccagaaggc cgcccatggg
     5041 cacgtccgca aggccttcaa gagccacgtc tctaccttga cagacctcca gccgtacatg
     5101 cgacagttcg tggctcacct gcaggagacc agcccgctga gggatgccgt cgtcatcgag
     5161 cagagctcct ccctgaatga ggccagcagt ggcctcttcg acgtcttcct acgcttcatg
     5221 tgccaccacg ccgtgcgcat caggggcaag tcctacgtcc agtgccaggg gatcccgcag
     5281 ggctccatcc tctccacgct gctctgcagc ctgtgctacg gcgacatgga gaacaagctg
     5341 tttgcgggga ttcggcggga cgggctgctc ctgcgtttgg tggatgattt cttgttggtg
     5401 acacctcacc tcacccacgc gaaaaccttc ctcaggaccc tggtccgagg tgtccctgag
     5461 tatggctgcg tggtgaactt gcggaagaca gtggtgaact tccctgtaga agacgaggcc
     5521 ctgggtggca cggcttttgt tcagatgccg gcccacggcc tattcccctg gtgcggcctg
     5581 ctgctggata cccggaccct ggaggtgcag agcgactact ccagctatgc ccggacctcc
     5641 atcagagcca gtctcacctt caaccgcggc ttcaaggctg ggaggaacat gcgtcgcaaa
     5701 ctctttgggg tcttgcggct gaagtgtcac agcctgtttc tggatttgca ggtgaacagc
     5761 ctccagacgg tgtgcaccaa catctacaag atcctcctgc tgcaggcgta caggtttcac
     5821 gcatgtgtgc tgcagctccc atttcatcag caagtttgga agaaccccac atttttcctg
     5881 cgcgtcatct ctgacacggc ctccctctgc tactccatcc tgaaagccaa gaacgcaggg
     5941 atgtcgctgg gggccaaggg cgccgccggc cctctgccct ccgaggccgt gcagtggctg
     6001 tgccaccaag cattcctgct caagctgact cgacaccgtg tcacctacgt gccactcctg
     6061 gggtcactca ggacagccca gacgcagctg agtcggaagc tcccggggac gacgctgact
     6121 gccctggagg ccgcagccaa cccggcactg ccctcagact tcaagaccat cctggactga
     6181 gtcgacatta attccggtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     6241 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     6301 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     6361 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     6421 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     6481 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     6541 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     6601 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
     6661 ggacgtggtt ttcctttgaa aaacacgatg ataataccat ggcttcgtac ccctgccatc
     6721 aacacgcgtc tgcgttcgac caggctgcgc gttctcgcgg ccatagcaac cgacgtacgg
     6781 cgttgcgccc tcgccggcag caagaagcca cggaagtccg cctggagcag aaaatgccca
     6841 cgctactgcg ggtttatata gacggtcctc acgggatggg gaaaaccacc accacgcaac
     6901 tgctggtggc cctgggttcg cgcgacgata tcgtctacgt acccgagccg atgacttact
     6961 ggcaggtgct gggggcttcc gagacaatcg cgaacatcta caccacacaa caccgcctcg
     7021 accagggtga gatatcggcc ggggacgcgg cggtggtaat gacaagcgcc cagataacaa
     7081 tgggcatgcc ttatgccgtg accgacgccg ttctggctcc tcatatcggg ggggaggctg
     7141 ggagctcaca tgccccgccc ccggccctca ccctcatctt cgaccgccat cccatcgccg
     7201 ccctcctgtg ctacccggcc gcgcgatacc ttatgggcag catgaccccc caggccgtgc
     7261 tggcgttcgt ggccctcatc ccgccgacct tgcccggcac aaacatcgtg ttgggggccc
     7321 ttccggagga cagacacatc gaccgcctgg ccaaacgcca gcgccccggc gagcggcttg
     7381 acctggctat gctggccgcg attcgccgcg tttacgggct gcttgccaat acggtgcggt
     7441 atctgcaggg cggcgggtcg tggcgggagg attggggaca gctttcgggg acggccgtgc
     7501 cgccccaggg tgccgagccc cagagcaacg cgggcccacg accccatatc ggggacacgt
     7561 tatttaccct gtttcgggcc cccgagttgc tggcccccaa cggcgacctg tacaacgtgt
     7621 ttgcctgggc cttggacgtc ttggccaaac gcctccgtcc catgcacgtc tttatcctgg
     7681 attacgacca atcgcccgcc ggctgccggg acgccctgct gcaacttacc tccgggatgg
     7741 tccagaccca cgtcaccacc cccggctcca taccgacgat ctgcgacctg gcgcgcacgt
     7801 ttgcccggga gatgggggag gctaactgaa acacggaagg ggtaccgaat tcctcgacgg
     7861 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7921 aggggggact ggaagggcta attcactccc aacgaagaca agatgggatc aattcaccat
     7981 gggaataact tcgtatagca tacattatac gaagttatgc tgctttttgc ttgtactggg
     8041 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8101 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8161 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     8221 atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata
     8281 aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag
     8341 catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt
     8401 tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg
     8461 aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca
     8521 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     8581 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     8641 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     8701 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     8761 agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     8821 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     8881 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     8941 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     9001 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     9061 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9121 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9181 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9241 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9301 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9361 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9421 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9481 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9541 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9601 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9661 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9721 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9781 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9841 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9901 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9961 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10021 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10081 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10141 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10201 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10261 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10321 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10381 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10441 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10501 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10561 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10621 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10681 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10741 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10801 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10861 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10921 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10981 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
    11041 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    11101 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    11161 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
    11221 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    11281 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    11341 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
    11401 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
    11461 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
    11521 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
    11581 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
    11641 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
    11701 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
    11761 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
    11821 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
    11881 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
    11941 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
    12001 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
    12061 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
    12121 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
    12181 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
    12241 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
    12301 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
    12361 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
    12421 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
    12481 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
    12541 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
    12601 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
    12661 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
    12721 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
    12781 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
    12841 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
    12901 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
    12961 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
    13021 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    13081 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    13141 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    13201 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    13261 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    13321 ccaattacct gt
//
