LOCUS       sequence-428738-        8966 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for expressing mTagBFP2 localizing to the nucleus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Reicher A, Reinis J, Ciobanu M, Ruzicka P, Malik M, Siklos M, 
            Kartysh V, Tomek T, Koren A, Rendeiro AF, Kubicek S
  TITLE     Pooled multicolour tagging for visualizing subcellular protein 
            dynamics.
  JOURNAL   Nat Cell Biol. 2024 May;26(5):745-756. doi: 
            10.1038/s41556-024-01407-w. Epub 2024 Apr 19.
  PUBMED    38641660
REFERENCE   2  (bases 1 to 8966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-024-01407-w"; journalName: "Nat Cell Biol"; date: 
            "2024-05"; volume: "26"; issue: "5"; pages: "745-756"
COMMENT     Sequence Label: lenti-NLS-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..8966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..57)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     248..269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(388..408)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        393..722
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      573..708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     635..654
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        770..817
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             836..1210
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1340..1461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1377..1396)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1431..1450
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1510..1526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1510..1526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1523..1545)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1534..1550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1558..1564,1565..1582,1583..1588))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1603..1624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1741..1758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1912..2500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1992..2011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2671..3462,3463..3531))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3294..3313
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3532..3636)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3711..3730)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3902..4281
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4283..4481
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4436..4456
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4499..4679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4726..4851
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5344..5577
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5762..5806
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6104..6221
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6314..6525
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6533..6542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6587..7294
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     misc_feature    7322..7910
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7375..7395)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7793..7804)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7913..7929)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7914..7930)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8435..8615
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8641..8658)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8647..8871
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8917..8966,1..379)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
       61 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      121 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      181 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      241 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      301 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      361 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
      421 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
      481 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      541 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
      601 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
      661 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
      721 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
      781 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
      841 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
      901 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
      961 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     1021 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     1081 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     1141 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     1201 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     1261 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1321 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     1381 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1441 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     1501 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1561 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1621 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1681 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1741 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1801 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1861 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1921 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1981 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2041 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2101 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2221 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2281 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2341 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2401 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2461 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2521 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2581 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2641 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2701 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2761 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2821 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2881 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2941 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3001 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3061 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3121 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3181 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3241 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3301 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3361 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3421 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3481 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3541 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3601 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3661 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     3721 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     3781 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     3841 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     3901 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     3961 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4021 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4081 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4141 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4201 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4261 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4321 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4381 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     4441 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
     4501 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4561 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4621 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4681 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     4741 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     4801 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     4861 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     4921 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     4981 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     5041 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     5101 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     5161 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     5221 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     5281 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     5341 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     5401 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     5461 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     5521 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     5581 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     5641 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     5701 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     5761 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     5821 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     5881 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     5941 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     6001 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     6061 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     6121 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     6181 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     6241 gcagagatcc agtttggtta attagctagc taggtcttga aaggagtggg aattggctcc
     6301 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6361 gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     6421 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6481 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggaccg gtgccaccat
     6541 gggtccacct agaaagaaaa ggaagatcgg aggtggctcg ggcggtgtgt ctaagggcga
     6601 agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca
     6661 tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
     6721 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt
     6781 cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca
     6841 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
     6901 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
     6961 aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     7021 cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct
     7081 gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata gatccaagaa
     7141 acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat
     7201 caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg
     7261 cgacctccct agcaaactgg ggcacaagct taattaagaa ttcgatatca agcttatcgg
     7321 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7381 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7441 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7501 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7561 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7621 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7681 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     7741 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7801 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7861 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     7921 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     7981 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     8041 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     8101 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     8161 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     8221 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     8281 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     8341 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     8401 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     8461 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8521 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8581 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     8641 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     8701 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     8761 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     8821 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     8881 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     8941 gcgcggcggg tgtggtggtt acgcgc
//
