LOCUS       sequence-429516-        9382 bp    DNA     circular SYN 15-APR-2025
DEFINITION  RADARSv3 driving luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Jiang K, Koob J, Chen XD, Krajeski RN, Zhang Y, Volf V, Zhou W, 
            Sgrizzi SR, Villiger L, Gootenberg JS, Chen F, Abudayyeh OO
  TITLE     Programmable eukaryotic protein synthesis with RNA sensors by 
            harnessing ADAR.
  JOURNAL   Nat Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 
            10.1038/s41587-022-01534-5.
  PUBMED    36302988
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 
            10.1038/s41587-022-01534-5."
COMMENT     Sequence Label: pKJ275
FEATURES             Location/Qualifiers
     source          1..9382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(151..156,157..174,175..181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     213..229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     213..229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    243..291
                     /note="synthetic polyadenylation signal"
     CDS             complement(292..1953)
                     /codon_start=1
                     /product="secreted Cypridina luciferase"
                     /label=CLuc
                     /note="contains 30 codon substitutions for efficient 
                     translation in mammalian cells"
                     /translation="MKTLILAVALVYCATVHCQDCPYEPDPPNTVPTSCEAKEGECIDS
                     SCGTCTRDILSDGLCENKPGKTCCRMCQYVIECRVEAAGWFRTFYGKRFQFQEPGTYVL
                     GQGTKGGDWKVSITLENLDGTKGAVLTKTRLEVAGDIIDIAQATENPITVNGGADPIIA
                     YPYTIGEVTIAVVEMPGFNITVIEFFKLIVIDILGGRSVRIAPDTANKGMISGLCGDLK
                     MMEDTDFTSDPEQLAIQPKINQEFDGCPLYGNPDDVAYCKGLLEPYKDSCRNPINFYYY
                     TISCAFARCMGGDERASHVLLDYRETCAAPETRGTCVLSGHTFYDTFDKARYQFQGPCK
                     EILMAADCFWNTWDVKVSHRNVDSYTEVEKVRIRKQSTVVELIVDGKQILVGGEAVSVP
                     YSSQNTSIYWQDGDILTTAILPEALVVKFNFKQLLVVHIRDPFDGKTCGICGNYNQDFS
                     DDSFDAEGACDLTPNPPGCTEEQKPEAERLCNSLFAGQSDLDQKCNVCHKPDRVERCMY
                     EYCLRGQQGFCDHAWEFKKECYIKHGDTLEVPDECK"
     primer_bind     complement(1978..1997)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1979..1997)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2042..2245)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2071..2091)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2246..2625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2739..3917
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2970..3908
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3865..3885
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3930..3939
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3936..4646
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4081..4099)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4349..4368
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_RNA        4775..4793
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             4950..5006
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5016..5570
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="GVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRGK
                     LPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQ
                     GGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQR
                     CATFASKIQGQVDKIKGAGGD"
     misc_feature    5571..6139
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5589..6141
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5733..5750)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5960..5979
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6153..6752
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6153..6172)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6649..6669
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6761..6985
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6995..7012)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6995..7011)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7004..7026)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7220..7239)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7339..7361
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7399..7417)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7485..7589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7590..7658,7659..8450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSGGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7808..7827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8621..9209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9110..9129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9363..9380
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
       61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      241 cacacacaaa aaaccaacac acagatgtaa tgaaaataaa gatattttat tttatttgca
      301 ttcatctggt acttctaggg tgtctccatg ctttatgtag cattctttct tgaactccca
      361 tgcgtggtca cagaaaccct gttgtcccct caggcaatac tcgtacatgc atcgttcgac
      421 acggtcaggc ttgtggcaca cgttacattt ctgatcaaga tcactttgac cggcgaagag
      481 actattgcag agtcgttcag cttcaggttt ctgttcttcg gtgcatcccg gtgggttggg
      541 ggtcagatca caggctcctt cagcatcaaa agaatcatca ctgaaatcct ggttgtagtt
      601 accgcaaata ccgcaagtct taccatcgaa tggatctcta atatgtacga cgagcagttg
      661 cttgaagttg aacttgacca ccagagcttc aggtaggatg gctgtagtca gtatgtcacc
      721 atcttgccag tagatggaag tgttctgaga gctgtacggg acggacacgg cttctcctcc
      781 aaccagaatc tgttttccat caacaatgag ttctactaca gtcgattgtt tcctgattcg
      841 tactttctct acttcagtgt aagagtcaac attcctgtgt gaaaccttca catcccaagt
      901 gttccagaaa cagtcggcgg ccataagaat ctccttgcag ggaccctgga attggtatct
      961 tgctttgtca aatgtatcgt agaaagtatg tccagacaaa acgcaggttc ctctagtttc
     1021 gggagcagcg cacgtctccc tgtagtcaag cagcacgtgt gaggctcgct cgtctccacc
     1081 catacagcgg gcgaaggcgc aggagatggt gtagtagtag aagttgatgg ggttgcggca
     1141 gctgtccttg tacggctcca gaagaccttt gcagtatgca acgtcatcag gatttccata
     1201 gagtggacaa ccgtcaaact cctggttgat cttaggctga atagcgagtt gttctggatc
     1261 tgaagtgaag tctgtatctt ccatcatttt aagatctcca cagaggccag agatcattcc
     1321 tttgtttgct gtgtctgggg cgattcttac agatcttcct ccgaggatgt cgatcacgat
     1381 cagtttgaag aactcaatga cggtgatgtt gaagcctggc atctcaacaa cagcgatggt
     1441 gacctcgccg atggtgtacg ggtaggcgat gatagggtca gctccaccgt ttacagtgat
     1501 gggattctca gtagcttgag cgatgtcaat gatgtctcca gccacttcca gtcttgtctt
     1561 ggtcagcaca gcccccttgg ttccatccag gttctccagg gtgatggaca ccttccagtc
     1621 gccgcccttg gttccttgac ccaacacgta tgtaccaggt tcctggaact ggaatctctt
     1681 tccatagaat gttctaaacc atcctgcggc ctctactctg cattcaatta catactgaca
     1741 cattcgacaa catgtttttc ctggtttatt ttcacacagt ccatctgata gtatgtctct
     1801 cgtgcaggtg ccacagctgc tatcaataca ttctccttct ttagcttcac aggaagttgg
     1861 aactgtgttt ggtggatcag gttcgtaagg acagtcctgg caatgaacag tggcgcagta
     1921 gactaatgca acggcaagaa ttaaggtctt catggtggcg gtaccaagct tgcgtctccc
     1981 tatagtgagt cgtattaatt tcgataagcc agtaagcagt gggttctcta gttagccaga
     2041 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     2101 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     2161 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     2221 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     2281 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     2341 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     2401 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     2461 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     2521 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     2581 accccgtaat tgattactat taataactag tcaataatca atgtcaacgc gtatatctgg
     2641 cccgtacatc gaagctgatg caaagatgga taaagtttta aacagagagg aatctttgca
     2701 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     2761 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2821 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2881 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2941 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     3001 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     3061 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     3121 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     3181 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     3241 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     3301 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     3361 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     3421 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     3481 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     3541 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     3601 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     3661 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     3721 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3781 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3841 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3901 catttcaggt gtcgtgacgt acgggatccg ccaccatggt gagcaagggc gaggaggata
     3961 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     4021 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     4081 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     4141 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     4201 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     4261 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     4321 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     4381 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     4441 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     4501 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     4561 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     4621 gcggcatgga cgagctgtac aagtagggca ggggaagtct tctaacatgc ggggacgtgg
     4681 aggaaaatcc cggcccctct agaactccaa aagaccagtg gtggttttcg acgcaggacc
     4741 accgcgtcgg caagtctcct cattggatcc agatacatga ggatcaccca tgtgcatcca
     4801 tctttttcag ccatctttag aaggttcagg ttgttttctg ccaggacgca ggaccaccgc
     4861 gtcctttgct gctttcacac aggttactca catggggatc acccaggtac aggtctcctt
     4921 tctcagggct gagagctagc ggaagcggag ctactaactt cagcctgctg aagcaggctg
     4981 gcgacgtgga ggagaaccct ggacctgtaa gtaagggagt caaagttctg tttgccctga
     5041 tctgcatcgc tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg
     5101 ccgtggccag caacttcgcg accacggatc tcgatgctga ccgcgggaag ttgcccggca
     5161 agaagctgcc gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca
     5221 ccaggggctg tctgatctgc ctgtcccaca tcaagtgcac gcccaagatg aagaagttca
     5281 tcccaggacg ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg
     5341 aggcgatcgt cgacattcct gagattcctg ggttcaagga cttggagccc atggagcagt
     5401 tcatcgcaca ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca
     5461 acgtgcagtg ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca
     5521 gcaagatcca gggtcaggtg gacaagatca agggggccgg tggtgactaa tccctccccc
     5581 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     5641 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     5701 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     5761 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     5821 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     5881 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     5941 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     6001 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     6061 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     6121 ttgaaaaaca cgatgataat atggccacac atatgaccga gtacaagccc acggtgcgcc
     6181 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6241 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6301 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6361 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6421 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6481 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6541 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6601 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6661 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     6721 cctggtgcat gacccgcaag cccggtgcct aagcggccgc ctgtgccttc tagttgccag
     6781 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6841 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6901 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6961 gctggggatg cggtgggctc tatggctcga attcactggc cgtcgtttta caacgtcgtg
     7021 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     7081 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7141 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7201 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     7261 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     7321 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     7381 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     7441 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7501 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7561 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7621 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7681 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7741 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7801 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7861 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7921 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7981 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8041 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8101 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8161 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8221 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8281 ataaatctgg agccggtgag cgtgggtctg gcggtatcat tgcagcactg gggccagatg
     8341 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8401 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8461 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8521 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8581 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8641 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8701 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8761 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8821 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8881 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8941 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9001 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9061 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9121 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9181 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9241 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9301 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9361 gcagcgagtc agtgagcgag ga
//
