LOCUS       sequence-430086-       11293 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of NPTX1-pHluorin for optical monitoring of presynaptic 
            release in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Pascual-Caro C, de Juan-Sanz J
  TITLE     Activity-driven trafficking of endogenous synaptic proteins through 
            proximity labeling
  JOURNAL   bioRxiv 2024.04.19.590083
REFERENCE   2  (bases 1 to 11293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.19.590083"
COMMENT     Sequence Label: CKII-NPTX1-pH
FEATURES             Location/Qualifiers
     source          1..11293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2888..2907)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    3332..3920
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3385..3405)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3803..3814)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3923..3939)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3924..3940)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4445..4625
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4651..4668)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4657..4881
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4927..5355
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5014..5033)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5224..5245
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5364..5384)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5369..5698
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5549..5684
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5611..5630
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5746..5793
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5812..6186
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6316..6437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6353..6372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6407..6426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6486..6502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6486..6502)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6499..6521)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6510..6526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6534..6540,6541..6558,6559..6564))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6579..6600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6717..6734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6888..7476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6968..6987)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7647..8438,8439..8507))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8270..8289
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8508..8612)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8687..8706)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8878..9257
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9259..9457
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9412..9432
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9475..9655
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9702..9827
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10320..10553
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10738..10782
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11080..11197
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(11242..11293,1..1237)
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
ORIGIN
        1 agaaaatgag atgggggtgc agagagcttc ctcagtgacc tgcccagggt cacatcagaa
       61 atgtcagagc tagaacttga actcagatta ctaatcttaa attccatgcc ttgggggcat
      121 gcaagtacga tatacagaag gagtgaactc attagggcag atgaccaatg agtttaggaa
      181 agaagagtcc agggcagggt acatctacac cacccgccca gccctgggtg agtccagcca
      241 cgttcacctc attatagttg cctctctcca gtcctacctt gacgggaagc acaagcagaa
      301 actgggacag gagccccagg agaccaaatc ttcatggtcc ctctgggagg atgggtgggg
      361 agagctgtgg cagaggcctc aggaggggcc ctgctgctca gtggtgacag ataggggtga
      421 gaaagcagac agagtcattc cgtcagcatt ctgggtctgt ttggtacttc ttctcacgct
      481 aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg agagctggaa agaaacaagg
      541 acagagacag aggccaagtc aaccagacca attcccagag gaagcaaaga aaccattaca
      601 gagactacaa gggggaaggg aaggagagat gaattagctt cccctgtaaa ccttagaacc
      661 cagctgttgc cagggcaacg gggcaatacc tgtctcttca gaggagatga agttgccagg
      721 gtaactacat cctgtctttc tcaaggacca tcccagaatg tggcacccac tagccgttac
      781 catagcaact gcctctttgc cccacttaat cccatcccgt ctgttaaaag ggccctatag
      841 ttggaggtgg gggaggtagg aagagcgatg atcacttgtg gactaagttt gttcgcatcc
      901 ccttctccaa ccccctcagt acatcaccct gggggaacag ggtccacttg ctcctgggcc
      961 cacacagtcc tgcagtattg tgtatataag gccagggcaa agaggagcag gttttaaagt
     1021 gaaaggcagg caggtgttgg ggaggcagtt accggggcaa cgggaacagg gcgtttcgga
     1081 ggtggttgcc atggggacct ggatgctgac gaaggctcgc gaggctgtga gcagccacag
     1141 tgccctgctc agaagcccca agctcgtcag tcaagccggt tctccgtttg cactcaggag
     1201 cacgggcagg cgagtggccc ctagttctgg gggcagcggg ggatccgcca ccatgctggc
     1261 cggccgcgcc gcacgcacct gtgcgctgct cgccctctgc ctcctgggca gtcgggccca
     1321 ggatttcggg ccgacccgct tcatctgcac ttcggtgccg gtggacgccg acatgtgtgc
     1381 cgcgtccgtg gccgcgggcg gcgcggagga gcttcggagc aatgtgctgc agctccggga
     1441 gaccgtgctg cagcagaagg agaccatcct cagccagaag gagaccatca gggagctgac
     1501 caccaagctt ggccgctgcg agagccagag caccctggac gcgggtccgg gcgaggccag
     1561 gtcgggcggc ggccgcaagc agcctggctc gggcaagaat acgatgggcg acctgtcccg
     1621 gactccggcc tcggagacgc tcagccaact cgggcaaact ttgcaatccc tcaaaacccg
     1681 actggagaac ctcgagcagt acagccgcct caattcttcc agccaaacca acagcctcaa
     1741 ggatctgctg cagagcaaga tcgatgacct ggagcggcag gtgctgtctc gagtgaacac
     1801 tctggaggag ggaaaagggg gccccaagaa cgacacagag gaaagggcca agatcgagag
     1861 cgccttgacc tccctacacc agcggatcag cgagctggag aaaggtcaga aagacaatcg
     1921 acccggagac aagtttcagc tgacattccc actgcggacc aactacatgt atgccaaggt
     1981 gaagaagagc ctgccggaga tgtacgcctt cactgtgtgc atgtggctca agtccagcgc
     2041 ggcacctgga gtgggcacgc ccttctccta tgctgtacct gggcaggcca acgagctggt
     2101 cctcatcgag tggggcaaca accccatgga gatactcatt aacgacaagg tggccaagct
     2161 gccctttgta atcaacgacg gcaagtggca ccacatatgc gttacctgga ccacccggga
     2221 cggggtctgg gaggcctatc aggatggcac ccagggaggc aatggagaga acctggcacc
     2281 ctatcaccct atcaaaccac agggtgtgct ggtgctaggc caggagcagg acactctagg
     2341 cggagggttt gatgccaccc aagcgtttgt gggtgagcta gcccatttca acatctggga
     2401 ccgcaagctg acccctgggg aggtgtacaa cttggccacc tgcagcagca aggccctctc
     2461 tggcaatgtc atcgcctggg ctgagtccca gatcgagatc tttggaggag ccaccaagtg
     2521 gacattcgag gcttgccgcc agatcaacag cggctctact agtggtggat ctggtggaac
     2581 cggcggctcc aaaggcgagg aactgtttac aggcgtggtg cctatcctgg tcgagctgga
     2641 tggggatgtg aacggccaca agttctccgt gtctggcgaa ggcgaagggg acgccacata
     2701 cggaaagctg accctgaagt tcatctgcac caccggcaag ctgcctgtgc cttggcctac
     2761 actggtcacc acactgacat acggcgtgca gtgcttcagc agataccccg accatatgaa
     2821 gaggcacgac ttcttcaaga gcgccatgcc tgagggctac gtgcaagaga ggaccatctt
     2881 ctttaaggac gacggcaact acaagaccag ggccgaagtg aagttcgagg gcgacaccct
     2941 ggtcaacagg atcgagctga agggaatcga cttcaaagag gacggcaaca tcctgggaca
     3001 caagttggag tacaactaca acgatcacca ggtctacatc atggccgata agcagaagaa
     3061 cggcatcaag gccaacttca agatccggca caacatcgag gacggcggag tgcagctggc
     3121 cgatcactat cagcagaaca cccctatcgg cgacggacct gtgctgctgc ctgataacca
     3181 ctacctgttt accaccagca cactgagcaa ggaccccaac gagaagaggg accacatggt
     3241 gctgttggag ttcgtgacag ccgccggaat cacacacgga atggacgagc tgtacaaatg
     3301 agatatcgaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     3361 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3421 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3481 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3541 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3601 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3661 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     3721 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     3781 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     3841 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3901 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     3961 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     4021 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     4081 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4141 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     4201 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     4261 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     4321 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     4381 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     4441 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4501 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4561 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4621 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     4681 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4741 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4801 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4861 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4921 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4981 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5041 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5101 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5161 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5221 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5281 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5341 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5401 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     5461 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5521 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5581 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     5641 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5701 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     5761 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     5821 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     5881 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     5941 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     6001 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     6061 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     6121 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     6181 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     6241 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     6301 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6361 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6421 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     6481 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6541 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6601 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6661 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6841 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7321 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7381 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7921 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7981 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8041 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8101 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8161 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8221 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8281 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8341 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8401 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8461 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8521 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8581 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8641 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     8701 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     8761 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     8821 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     8881 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     8941 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     9001 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     9061 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     9121 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     9181 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     9241 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     9301 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     9361 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     9421 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
     9481 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9541 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9601 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     9661 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     9721 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     9781 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     9841 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     9901 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     9961 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    10021 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    10081 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    10141 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
    10201 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    10261 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    10321 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    10381 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    10441 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
    10501 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    10561 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    10621 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
    10681 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    10741 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    10801 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    10861 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
    10921 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
    10981 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
    11041 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
    11101 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
    11161 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
    11221 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
    11281 cttctgattt tct
//
