LOCUS       sequence-430268-        1050 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression of wild type TEX264, C-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Fielden J, Wiseman K, Torrecilla I, Li S, Hume S, Chiang SC, 
            Ruggiano A, Narayan Singh A, Freire R, Hassanieh S, Domingo E, 
            Vendrell I, Fischer R, Kessler BM, Maughan TS, El-Khamisy SF, 
            Ramadan K
  TITLE     TEX264 coordinates p97- and SPRTN-mediated resolution of 
            topoisomerase 1-DNA adducts.
  JOURNAL   Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w.
  PUBMED    32152270
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-9"; volume: "11(1):1274. doi"; pages: " 
            10.1038/s41467-020-15000-w"
COMMENT     Sequence Label: pET21a-TEX264_wt-His6
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             999..1016
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgtcg gacctgctac
       61 tactgggcct gattgggggc ctgactctct tactgctgct gacgctgctg gcctttgccg
      121 ggtactcagg gctactggct ggggtggaag tgagtgctgg gtcacccccc atccgcaacg
      181 tcactgtggc ctacaagttc cacatggggc tctatggtga gactgggcgg cttttcactg
      241 agagctgcag catctctccc aagctccgct ccatcgctgt ctactatgac aacccccaca
      301 tggtgccccc tgataagtgc cgatgtgccg tgggcagcat cctgagtgaa ggtgaggaat
      361 cgccctcccc tgagctcatc gacctctacc agaaatttgg cttcaaggtg ttctccttcc
      421 cggcacccag ccatgtggtg acagccacct tcccctacac caccattctg tccatctggc
      481 tggctacccg ccgtgtccat cctgccttgg acacctacat caaggagcgg aagctgtgtg
      541 cctatcctcg gctggagatc taccaggaag accagatcca tttcatgtgc ccactggcac
      601 ggcagggaga cttctatgtg cctgagatga aggagacaga gtggaaatgg cgggggcttg
      661 tggaggccat tgacacccag gtggatggca caggagctga cacaatgagt gacacgagtt
      721 ctgtaagctt ggaagtgagc cctggcagcc gggagacttc agctgccaca ctgtcacctg
      781 gggcgagcag ccgtggctgg gatgacggtg acacccgcag cgagcacagc tacagcgagt
      841 caggtgccag cggctcctct tttgaggagc tggacttgga gggcgagggg cccttagggg
      901 agtcacggct ggaccctggg actgagcccc tggggactac caagtggctc tgggagccca
      961 ctgcccctga gaagggcaag gaggcggccg cactcgagca ccaccaccac caccactgag
     1021 atccggctgc taacaaagcc cgaaaggaag
//
