LOCUS       sequence-43057-e        9528 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS2.2 (FF573)
FEATURES             Location/Qualifiers
     source          1..9528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(588..840)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(860..1654)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(971..990)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1581..1600
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1688..1867)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     2099..2121
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2113..2130
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2114..2130
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2140..2159
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2140..2158
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2183..2199
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2184..2200
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2230..2248
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2230..2247
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(2281..2988)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4388..4733
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4676..4696
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    4780..4904
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4929..4934,4935..4952,4953..4959)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5013..5672
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5079..5098)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6014..6319
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6198..6217
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6360..6484)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6491..7132
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        complement(7154..7172)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7193..7209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7193..7209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7206..7228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7217..7233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7241..7247,7248..7266,7267..7272))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7287..7308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7425..7442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7547..7571
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7662..8250)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7742..7761)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8421..9209)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(9500..9528,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cagcttgcat gccggtcgat ctagtaacat
      601 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
      661 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
      721 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
      781 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
      841 tgcttgactc taggggtcat cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
      901 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
      961 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     1021 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     1081 aggcatcgcc ctgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg
     1141 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     1201 gaccggcttc catccgagta cgtcctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     1261 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     1321 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     1381 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     1441 tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt
     1501 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     1561 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     1621 gagaacctgc gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga gagtgaatat
     1681 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag
     1741 aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg
     1801 acgtatgtgc ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt
     1861 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa
     1921 cgtgactccc ttaattctcc gctcatgatc gataacatta acgcttacaa tttccattcg
     1981 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     2041 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     2101 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     2161 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
     2221 ctgcagccca tttaggtgac actatagaat atcactagta agctagcttg catgccggtc
     2281 ctgctgagcc tcgacatgtt gtcgcaaaat tcgccctgga cccgcccaac gatttgtcgt
     2341 cactgtcaag gtttgacctg cacttcattt ggggcccaca tacaccaaaa aaatgctgca
     2401 taattctcgg ggcagcaagt cggttacccg gccgccgtgc tggaccgggt tgaatggtgc
     2461 ccgtaacttt cggtagagcg gacggccaat actcaacttc aaggaatctc acccatgcgc
     2521 gccggcgggg aaccggagtt cccttcagtg agcgttatta gttcgccgct cggtgtgtcg
     2581 tagatactag cccctggggc acttttgaaa tttgaataag atttatgtaa tcagtctttt
     2641 aggtttgacc ggttctgccg ctttttttaa aattggattt gtaataataa aacgcaattg
     2701 tttgttattg tggcgctcta tcatagatgt cgctataaac ctattcagca caatatattg
     2761 ttttcatttt aatattgtac atataagtag tagggtacaa tcagtaaatt gaacggagaa
     2821 tattattcat aaaaatacga tagtaacggg tgatatattc attagaatga accgaaaccg
     2881 gcggtaagga tctgagctac acatgctcag gttttttaca acgtgcacaa cagaattgaa
     2941 agcaaatatc atgcgatcat aggcgtctcg catatctcat taaagcaggg atcccgcatc
     3001 agatctatca aacacataga tttacgaaga tctctaacat taaatcatca gagagataag
     3061 tttagggagc aagctctttc gcttacacag agaatcacag aggaaagaga ctttaagaaa
     3121 actacaactt ttttgaccac tgtgatttct ctctgcaagc gaaagtactt aatcaccaac
     3181 acagtagatt gatatatttg ggtttatttg tgagaagaag aattcactag tggatcccgc
     3241 actcgagtgt taatcagaaa aactcagatt aatctacaaa ttcgatcgca caaactagaa
     3301 actaacacca gatctagata gaaatcacaa atcgaagagt aattattcga caaaactcaa
     3361 attatttgaa caaatcggat gatatctatg aaaccctaat cgagaattaa gatgatatct
     3421 aacgatcaaa cccagaaaat cgtcttcgat ctaagattaa cagaatctaa accaaagaac
     3481 atatacgaaa ttgggatcga acgaaaacaa aatcgaagat tttgagagaa taaggaacac
     3541 agaaatttac cttgatcacg gtagagagaa ttgagagaaa gtttttaaga ttttgagaaa
     3601 ttgaaatctg aattgtgaag aagaagagct ctttgggtat tgttttatag aagaagaaga
     3661 agaaaagacg aggacgacta ggtcacgaga aagctaaggc ggtgaagcaa tagctaataa
     3721 taaaatgaca cgtgtattga gcgttgttta cacgcaaagt tgtttttggc taattgcctt
     3781 atttttaggt tgaggaaaag tatttgtgct ttgagttgat aaacacgact cgtgtgtgcc
     3841 ggctgcaacc actttgacgc cgtttattac tgactcgtcg gggggatcca ttcggtcccc
     3901 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
     3961 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
     4021 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
     4081 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
     4141 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa
     4201 aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg
     4261 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
     4321 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
     4381 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
     4441 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
     4501 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
     4561 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     4621 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     4681 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga acacggggga
     4741 cgagctgtga tttttctcta caagcgaatc tagaggatca caagtttgta caaaaaagct
     4801 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     4861 cagactacat aatactgtaa aacacaacat atccagtcat attggcggcc ccattaggca
     4921 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggagc
     4981 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
     5041 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     5101 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     5161 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     5221 atccggagtt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     5281 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     5341 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     5401 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     5461 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     5521 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     5581 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     5641 agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg agccggctta ctaaaagcca
     5701 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     5761 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
     5821 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     5881 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     5941 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     6001 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     6061 gtttgtggat gtacagagtg atattattga cacgcccggc cgacggatgg tgatccccct
     6121 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     6181 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     6241 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     6301 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg cacctcgacc
     6361 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     6421 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     6481 tggtgatcct agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca
     6541 tcagtttcat tgcgcacaca ccagaatcct actgagtttg agtattatgg cattgggaaa
     6601 actgtttttc ttgtaccatt tgttgtgctt gtaatttact gtgtttttta ttcggttttc
     6661 gctatcgaac tgtgaaatgg aaatggatgg agaagagtta atgaatgata tggtcctttt
     6721 gttcattctc aaattaatat tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa
     6781 attataagag atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta
     6841 atgaccgaag ttaatatgag gagtaaaaca cttgtagttg taccattatg cttattcact
     6901 aggcaacaaa tatattttca gacctagaaa agctgcaaat gttactgaat acaagtatgt
     6961 cctcttgtgt tttagacatt tatgaacttt cctttatgta attttccaga atccttgtca
     7021 gattctaatc attgctttat aattatagtt atactcatgg atttgtagtt gagtatgaaa
     7081 atatttttta atgcatttta tgacttgcca attgattgac aacatgcatc aatcggagct
     7141 ccagcttttg ttccctttag tgagggttaa ttccgagctt ggcgtaatca tggtcatagc
     7201 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaaghc
     7261 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7321 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7381 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7441 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7501 ttatccacag aatcagggga taacgcagga aagaacatga aggccttgac aggatatatt
     7561 ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc tcatgtgagc aaaaggccag
     7621 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7681 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7741 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7801 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7861 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7921 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7981 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8041 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8101 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaag aagagttggt
     8161 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8221 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8281 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8341 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8401 gtgtaacatt ggtctagtga ttatttgccg actaccttgg tgatctcgcc tttcacgtag
     8461 tgaacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttg tccaagataa
     8521 gcctgcctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     8581 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     8641 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     8701 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     8761 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     8821 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     8881 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     8941 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
     9001 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     9061 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     9121 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     9181 gatacttcgg cgatcaccgc ttccctcata acaccccttg tattactgtt tatgtaagca
     9241 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     9301 tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc
     9361 acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac
     9421 tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat
     9481 ggggcgattc aggcgatccc catccaacag cccgccgtcg agcgggct
//
