LOCUS       sequence-43059-e        9506 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS3.2 (FF574)
FEATURES             Location/Qualifiers
     source          1..9506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(588..840)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(860..1654)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(971..990)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1581..1600
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1688..1867)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     2099..2121
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2113..2130
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2114..2130
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2140..2159
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2140..2158
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2183..2199
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2184..2200
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2230..2248
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2230..2247
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(2281..2988)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4388..4733
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4676..4696
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    4758..4882
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4907..4912,4913..4930,4931..4937)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4991..5650
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5057..5076)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5992..6297
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6176..6195
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6338..6462)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6469..7110
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        complement(7132..7150)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7171..7187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7171..7187)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7184..7206)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7195..7211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7219..7225,7226..7244,7245..7250))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7265..7286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7403..7420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7525..7549
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7640..8228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7720..7739)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8399..9187)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(9478..9506,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cagcttgcat gccggtcgat ctagtaacat
      601 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
      661 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
      721 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
      781 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
      841 tgcttgactc taggggtcat cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
      901 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
      961 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     1021 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     1081 aggcatcgcc ctgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg
     1141 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     1201 gaccggcttc catccgagta cgtcctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     1261 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     1321 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     1381 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     1441 tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt
     1501 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     1561 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     1621 gagaacctgc gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga gagtgaatat
     1681 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag
     1741 aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg
     1801 acgtatgtgc ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt
     1861 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa
     1921 cgtgactccc ttaattctcc gctcatgatc gataacatta acgcttacaa tttccattcg
     1981 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     2041 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     2101 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     2161 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
     2221 ctgcagccca tttaggtgac actatagaat atcactagta agctagcttg catgccggtc
     2281 ctgctgagcc tcgacatgtt gtcgcaaaat tcgccctgga cccgcccaac gatttgtcgt
     2341 cactgtcaag gtttgacctg cacttcattt ggggcccaca tacaccaaaa aaatgctgca
     2401 taattctcgg ggcagcaagt cggttacccg gccgccgtgc tggaccgggt tgaatggtgc
     2461 ccgtaacttt cggtagagcg gacggccaat actcaacttc aaggaatctc acccatgcgc
     2521 gccggcgggg aaccggagtt cccttcagtg agcgttatta gttcgccgct cggtgtgtcg
     2581 tagatactag cccctggggc acttttgaaa tttgaataag atttatgtaa tcagtctttt
     2641 aggtttgacc ggttctgccg ctttttttaa aattggattt gtaataataa aacgcaattg
     2701 tttgttattg tggcgctcta tcatagatgt cgctataaac ctattcagca caatatattg
     2761 ttttcatttt aatattgtac atataagtag tagggtacaa tcagtaaatt gaacggagaa
     2821 tattattcat aaaaatacga tagtaacggg tgatatattc attagaatga accgaaaccg
     2881 gcggtaagga tctgagctac acatgctcag gttttttaca acgtgcacaa cagaattgaa
     2941 agcaaatatc atgcgatcat aggcgtctcg catatctcat taaagcaggg atcccgcatc
     3001 agatctatca aacacataga tttacgaaga tctctaacat taaatcatca gagagataag
     3061 tttagggagc aagctctttc gcttacacag agaatcacag aggaaagaga ctttaagaaa
     3121 actacaactt ttttgaccac tgtgatttct ctctgcaagc gaaagtactt aatcaccaac
     3181 acagtagatt gatatatttg ggtttatttg tgagaagaag aattcactag tggatcccgc
     3241 actcgagtgt taatcagaaa aactcagatt aatctacaaa ttcgatcgca caaactagaa
     3301 actaacacca gatctagata gaaatcacaa atcgaagagt aattattcga caaaactcaa
     3361 attatttgaa caaatcggat gatatctatg aaaccctaat cgagaattaa gatgatatct
     3421 aacgatcaaa cccagaaaat cgtcttcgat ctaagattaa cagaatctaa accaaagaac
     3481 atatacgaaa ttgggatcga acgaaaacaa aatcgaagat tttgagagaa taaggaacac
     3541 agaaatttac cttgatcacg gtagagagaa ttgagagaaa gtttttaaga ttttgagaaa
     3601 ttgaaatctg aattgtgaag aagaagagct ctttgggtat tgttttatag aagaagaaga
     3661 agaaaagacg aggacgacta ggtcacgaga aagctaaggc ggtgaagcaa tagctaataa
     3721 taaaatgaca cgtgtattga gcgttgttta cacgcaaagt tgtttttggc taattgcctt
     3781 atttttaggt tgaggaaaag tatttgtgct ttgagttgat aaacacgact cgtgtgtgcc
     3841 ggctgcaacc actttgacgc cgtttattac tgactcgtcg gggggatcca ttcggtcccc
     3901 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
     3961 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
     4021 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
     4081 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
     4141 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa
     4201 aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg
     4261 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
     4321 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
     4381 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
     4441 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
     4501 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
     4561 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     4621 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     4681 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga acacggggga
     4741 cgagcttcta gaggatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     4801 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     4861 cacaacatat ccagtcatat tggcggcccc attaggcacc ccaggcttta cactttatgc
     4921 ttccggctcg tataatgtgt ggattttgag ttaggagccg tcgagatttt caggagctaa
     4981 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     5041 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     5101 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     5161 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggagttcc gtatggcaat
     5221 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     5281 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     5341 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     5401 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     5461 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     5521 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     5581 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     5641 cggggcgtaa acgcgtggag ccggcttact aaaagccaga taacagtatg cgtatttgcg
     5701 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     5761 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     5821 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     5881 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     5941 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     6001 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     6061 attattgaca cgcccggccg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     6121 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     6181 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     6241 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     6301 tcaggctccc ttatacacag ccagtctgca cctcgaccat agtgactgga tatgttgtgt
     6361 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     6421 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatcctag ctttcgttcg
     6481 tatcatcggt ttcgacaacg ttcgtcaagt tcaatgcatc agtttcattg cgcacacacc
     6541 agaatcctac tgagtttgag tattatggca ttgggaaaac tgtttttctt gtaccatttg
     6601 ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa
     6661 atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta
     6721 tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat
     6781 tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga
     6841 gtaaaacact tgtagttgta ccattatgct tattcactag gcaacaaata tattttcaga
     6901 cctagaaaag ctgcaaatgt tactgaatac aagtatgtcc tcttgtgttt tagacattta
     6961 tgaactttcc tttatgtaat tttccagaat ccttgtcaga ttctaatcat tgctttataa
     7021 ttatagttat actcatggat ttgtagttga gtatgaaaat attttttaat gcattttatg
     7081 acttgccaat tgattgacaa catgcatcaa tcggagctcc agcttttgtt ccctttagtg
     7141 agggttaatt ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7201 tccgctcaca attccacaca acatacgagc cggaaghcat aaagtgtaaa gcctggggtg
     7261 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7321 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7381 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7441 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7501 acgcaggaaa gaacatgaag gccttgacag gatatattgg cgggtaaact aagtcgctgt
     7561 atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7621 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7681 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7741 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7801 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7861 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7921 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7981 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8041 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8101 ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc ggcaaacaaa
     8161 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8221 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8281 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8341 attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg tctagtgatt
     8401 atttgccgac taccttggtg atctcgcctt tcacgtagtg aacaaattct tccaactgat
     8461 ctgcgcgcga ggccaagcga tcttcttgtc caagataagc ctgcctagct tcaagtatga
     8521 cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg
     8581 cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct
     8641 catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa
     8701 atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa
     8761 cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct
     8821 cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag
     8881 ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga
     8941 gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc
     9001 gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct
     9061 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat
     9121 ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt
     9181 ccctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     9241 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt
     9301 gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag
     9361 accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc
     9421 tcatcaaccg tggctccctc actttctggc tggatgatgg ggcgattcag gcgatcccca
     9481 tccaacagcc cgccgtcgag cgggct
//
