LOCUS       sequence-430649-       11148 bp    DNA     circular SYN 17-JUL-2025
DEFINITION  Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, 
            followed by IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Saito Y, Chapple RH, Lin A, Kitano A, Nakada D
  TITLE     AMPK Protects Leukemia-Initiating Cells in Myeloid Leukemias from 
            Metabolic Stress in the Bone Marrow.
  JOURNAL   Cell Stem Cell. 2015 Sep 29. pii: S1934-5909(15)00374-4. doi: 
            10.1016/j.stem.2015.08.019.
  PUBMED    26440282
REFERENCE   2  (bases 1 to 11148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Sep 29. pii: S1934-5909(15)00374-4. doi: 
            10.1016/j.stem.2015.08.019."
COMMENT     Sequence Label: pMIG-FLAG-MLL-AF9
FEATURES             Location/Qualifiers
     source          1..11148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     377..396
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             596..1112
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    1176..1517
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1584..2000
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1930..1952
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1968..1984
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             2031..2054
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(6604..7178,7179..7181,7182..7190)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6771..6788)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6998..7017
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7191..7193,7194..7196,7197..7910)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7236..7257)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7497..7516)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7844..7865
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             8060..8574
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8767..8783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8791..8797,8798..8815,8816..8821))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8836..8857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8974..8991)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9145..9733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9225..9244)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9904..10695,10696..10764))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10527..10546
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10765..10869)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10937..10955
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10993..11015)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11115..11134
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc
      241 aacagtccsm cggcncamgg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
      301 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
      361 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
      421 cggtgatgcc ggccacgatg cgtccggcgt agaggcgatt agtccaattt gttaaagaca
      481 ggatatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag
      541 agtacgagcc atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
      601 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
      661 aaatacataa ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa
      721 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
      781 gatggtcccc agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca
      841 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
      901 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
      961 actcggcgcg ccagtcctcc gatagactgc gtcgcccggg tacccgtatt cccaataaag
     1021 cctcttgctg tttgcatccg aatcgtggac tcgctgatcc ttgggagggt ctcctcagat
     1081 tgattgactg cccacctcgg gggtctttca tttggaggtt ccaccgagat ttggagaccc
     1141 ctgcccaggg accaccgacc cccccgccgg gaggtaagct ggccagcggt cgtttcgtgt
     1201 ctgtctctgt ctttgtgcgt gtttgtgccg gcatctaatg tttgcgcctg cgtctgtact
     1261 agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac
     1321 ccggccgcaa ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct
     1381 gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg
     1441 agaacctaaa acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc
     1501 gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     1561 ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag
     1621 gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac
     1681 gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg
     1741 gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc
     1801 atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc
     1861 ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc
     1921 cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc
     1981 tcactccttc tctaggcgcc ggaattagat ctctcgaggt taattccatg gactacaagg
     2041 acgacgatga caagggcaat tccgcgaaca tggcgcacag ctgtcggtgg cgcttccccg
     2101 cccgacccgg gaccaccggg ggcggcggcg gcggggggcg ccggggccta gggggcgccc
     2161 cgcggcaacg cgtcccggcc ctgctgcttc cccccgggcc cccggtcggc ggtggcggcc
     2221 ccggggcgcc cccctccccc ccggctgtgg cggccgcggc ggcggcggcg ggaagcagcg
     2281 gggctggggt tccaggggga gcggccgccg cctcagcagc ctcctcgtcg tccgcctcgt
     2341 cttcgtcttc gtcatcgtcc tcagcctctt cagggccggc cctgctccgg gtgggcccgg
     2401 gcttcgacgc ggcgctgcag gtctcggccg ccatcggcac caacctgcgc cggttccggg
     2461 ccgtgtttgg ggagagcggc gggggaggcg gcagcggaga ggatgagcaa ttcttaggtt
     2521 ttggctcaga tgaagaagtc agagtgcgaa gtcccacaag gtctccttca gttaaaacta
     2581 gtcctcgaaa acctcgtggg agacctagaa gtggctctga ccgaaattca gctatcctct
     2641 cagatccatc tgtgttttcc cctctaaata aatcagagac caaatctgga gataagatca
     2701 agaagaaaga ttctaaaagt atagaaaaga agagaggaag acctcccacc ttccctggag
     2761 taaaaatcaa aataacacat ggaaaggaca tttcagagtt accaaaggga aacaaagaag
     2821 atagcctgaa aaaaattaaa aggacacctt ctgctacgtt tcagcaagcc acaaagatta
     2881 aaaaattaag agcaggtaaa ctctctcctc tcaagtctaa gtttaagaca gggaagcttc
     2941 aaataggaag gaagggggta caaattgtac gacggagagg aaggcctcca tcaacagaaa
     3001 ggataaagac cccttcgggt ctcctcatta attctgaact ggaaaagccc cagaaagtcc
     3061 ggaaagacaa ggaaggaaca cctccactta caaaagaaga taagacagtt gtcagacaaa
     3121 gccctcgaag gattaagcca gttaggatta ttccttcttc aaaaaggaca gatgcaacca
     3181 ttgctaagca actcttacag agggcaaaaa agggggctca aaagaaaatt gaaaaagaag
     3241 cagctcagct gcagggaaga aaggtgaaga cacaggtcaa aaatattcga cagttcatca
     3301 tgcctgttgt cagtgctatc tcctcgcgga tcattaagac ccctcggcgg tttatagagg
     3361 atgaggatta tgaccctcca attaaaattg cccgattaga gtctacaccg aatagtagat
     3421 tcagtgcccc gtcctgtgga tcttctgaaa aatcaagtgc agcttctcag cactcctctc
     3481 aaatgtcttc agactcctct cgatctagta gccccagtgt tgatacctcc acagactctc
     3541 aggcttctga ggagattcag gtacttcctg aggagcggag cgatacccct gaagttcatc
     3601 ctccactgcc catttcccag tccccagaaa atgagagtaa tgataggaga agcagaaggt
     3661 attcagtgtc ggagagaagt tttggatcta gaacgacgaa aaaattatca actctacaaa
     3721 gtgcccccca gcagcagacc tcctcgtctc cacctccacc tctgctgact ccaccgccac
     3781 cactgcagcc agcctccagt atctctgacc acacaccttg gcttatgcct ccaacaatcc
     3841 ccttagcatc accatttttg cctgcttcca ctgctcctat gcaagggaag cgaaaatcta
     3901 ttttgcgaga accgacattt aggtggactt ctttaaagca ttctaggtca gagccacaat
     3961 acttttcctc agcaaagtat gccaaagaag gtcttattcg caaaccaata tttgataatt
     4021 tccgaccccc tccactaact cccgaggacg ttggctttgc atctggtttt tctgcatctg
     4081 gtaccgctgc ttcagcccga ttgttttcgc cactccattc tggaacaagg tttgatatgc
     4141 acaaaaggag ccctcttctg agagctccaa gatttactcc aagtgaggct cactctagaa
     4201 tatttgagtc tgtaaccttg cctagtaatc gaacttctgc tggaacatct tcttcaggag
     4261 tatccaatag aaaaaggaaa agaaaagtgt ttagtcctat tcgatctgaa ccaagatctc
     4321 cttctcactc catgaggaca agaagtggaa ggcttagtag ttctgagctc tcacctctca
     4381 cccccccgtc ttctgtctct tcctcgttaa gcatttctgt tagtcctctt gccactagtg
     4441 ccttaaaccc aacttttact tttccttctc attccctgac tcagtctggg gaatctgcag
     4501 agaaaaatca gagaccaagg aagcagacta gtgctccggc agagccattt tcatcaagta
     4561 gtcctactcc tctcttccct tggtttaccc caggctctca gactgaaaga gggagaaata
     4621 aagacaaggc ccccgaggag ctgtccaaag atcgagatgc tgacaagagc gtggagaagg
     4681 acaagagtag agagagagac cgggagagag aaaaggagaa taagcgggag tcaaggaaag
     4741 agaaaaggaa aaagggatca gaaattcaga gtagttctgc tttgtatcct gtgggtaggg
     4801 tttccaaaga gaaggttgtt ggtgaagatg ttgccacttc atcttctgcc aaaaaagcaa
     4861 cagggcggaa gaagtcttca tcacatgatt ctgggactga tattacttct gtgactcttg
     4921 gggatacaac agctgtcaaa accaaaatac ttataaagaa agggagagga aatctggaaa
     4981 aaaccaactt ggacctcggc ccaactgccc catccctgga gaaggagaaa accctctgcc
     5041 tttccactcc ttcatctagc actgttaaac attccacttc ctccataggc tccatgttgg
     5101 ctcaggcaga caagcttcca atgactgaca agagggttgc cagcctccta aaaaaggcca
     5161 aagctcagct ctgcaagatt gagaagagta agagtcttaa acaaaccgac cagcccaaag
     5221 cacagggtca agaaagtgac tcatcagaga cctctgtgcg aggaccccgg attaaacatg
     5281 tctgcagaag agcagctgtt gcccttggcc gaaaacgagc tgtgtttcct gatgacatgc
     5341 ccaccctgag tgccttacca tgggaagaac gagaaaagat tttgtcttcc atggggaatg
     5401 atgacaagtc atcaattgct ggctcagaag atgctgaacc tcttgctcca cccatcaaac
     5461 caattaaacc tgtcactaga aacaaggcac cccaggaacc tccagtaaag aaaggacgtc
     5521 gatcgaggcg gtgtgggcag tgtcccggct gccaggtgcc tgaggactgt ggtgtttgta
     5581 ctaattgctt agataagccc aagtttggtg gtcgcaatat aaagaagcag tgctgcaaga
     5641 tgagaaaatg tcagaatcta caatggatgc cttccaaagc ctacctgcag aagcaagcta
     5701 aagctgtgaa aaagaaagag aaaaagtcta agaccagtga aaagaaagac agcaaagaga
     5761 gcagtgttgt gaagaacgtg gtggactcta gtcagaaacc taccccatca gcaagagagg
     5821 atcctgcccc aaagaaaagc agtagtgagc ctcctccacg aaagcccgtc gaggaaaaga
     5881 gtgaagaagg gaatgtctcg gcccctgggc ctgaatccaa acaggccacc actccagctt
     5941 ccaggaagtc aagcaagcag gtctcccagc cagcactggt catcccgcct cagccaccta
     6001 ctacaggacc gccaagaaaa gaagttccca aaaccactcc tagtgagccc aagaaaaagc
     6061 agcctccacc accagaatca ggtccagagc agagcaaaca gaaaaaagtg gctccccgcc
     6121 caagtatccc tgtaaaacaa aaaccaaaag aaaaggaaaa accacctccg gtcaataagc
     6181 aggagaatgc aggcactttg aacatcctca gcactctctc caatggcaat agttctaagc
     6241 aaaaaattcc agcagatggg tcgcgaagta tacacacgtg tcttgaagtg aaaagtccaa
     6301 taaagcaaag caaatcagat aagcaaataa agaatggtga atgtgacaag gcatacctag
     6361 atgaactggt agagcttcac agaaggttaa tgacattgag agaaagacac attctgcagc
     6421 agatcgtgaa ccttatagaa gaaactggac actttcatat cacaaacaca acatttgatt
     6481 ttgatctttg ctcgctggac aaaaccacag tccgtaaact acagagttac ctggaaacat
     6541 ctggaacatc ctgactcgag ggatccgggc cctctagatg cggccgcatg catacgaatt
     6601 ccgccccyct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     6661 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     6721 ccggaaacct ggccctgtct tcttgacgag cattcctagg gctctttccc ctctcgccaa
     6781 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     6841 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     6901 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     6961 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     7021 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     7081 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     7141 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca
     7201 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     7261 acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac ggcaagctga
     7321 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     7381 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     7441 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7501 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7561 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7621 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     7681 cgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     7741 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     7801 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     7861 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa tgaattaatt
     7921 aagaattatc accgcttcta ttcagccagt aaggcctgtc tttaatggcc tccggcatga
     7981 gacactttct agagtcgacc tgcagccaag cttatcgata aaataaaaga ttttatttag
     8041 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     8101 aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg
     8161 ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct
     8221 gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc
     8281 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
     8341 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
     8401 aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc
     8461 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
     8521 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatgggtaa
     8581 cagtttcttg aagttggaga acaacattct gagggtagga gtcgaatatt aagtaatcct
     8641 gactcaatta gccactgttt tgaatccaca tactccaata ctcctgaaat agttcattat
     8701 ggacagcgca gmwmswgmwr krgwkmattr tggacagcgc agaaagagct ggggagaatt
     8761 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8821 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8881 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8941 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9001 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9061 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9121 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9181 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9601 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9661 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9721 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9781 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9841 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9901 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9961 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10021 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10081 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10141 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10201 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10261 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10321 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10381 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10441 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10501 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10561 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10621 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10681 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10741 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10801 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10861 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10921 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10981 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    11041 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    11101 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcacca
//
