LOCUS       sequence-430697-        9183 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal 
            bipartite SV40 NLS, along with puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9183)
  TITLE     Chavez Lab CRISPR Activator Plasmids 2024
REFERENCE   2  (bases 1 to 9183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1
FEATURES             Location/Qualifiers
     source          1..9183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..45
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    96..684
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(149..169)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(567..578)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             756..989
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1067..1188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1104..1123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1158..1177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1228..1363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1383..1402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1384..1402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1412..1429)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1412..1428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1421..1443)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1570..2025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1657..1676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1867..1888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2051..2155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2156..2224,2225..3016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2374..2393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3187..3775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3676..3695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3929..3946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4063..4084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4099..4104,4105..4122,4123..4129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4137..4153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4142..4164
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4161..4177
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4161..4177
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4196..4216
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4198..4216
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4244..4470
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4471..4651
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4698..4823
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5316..5549
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5734..5778
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6639..7046
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             7107..7703
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7107..7126)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7603..7623
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7716..7772
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7809..7829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8229..8771
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             8796..9167
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
ORIGIN
        1 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
       61 accggttaag tcgacaatca acgcgttaag tcgacaatca acctctggat tacaaaattt
      121 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
      181 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
      241 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
      301 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
      361 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
      421 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
      481 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
      541 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
      601 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
      661 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
      721 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
      781 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
      841 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      901 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      961 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     1021 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     1081 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1141 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     1201 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     1261 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     1321 tctgagctat tccagaagta gtgaggaggc ttttttggag gccaagggac gtacccaatt
     1381 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     1441 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     1501 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1561 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1621 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     1681 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     1741 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     1801 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     1861 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     1921 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     1981 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     2041 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     2101 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2161 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     2221 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     2281 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2341 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2401 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2461 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2521 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2581 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2641 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2701 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2761 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2821 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     2881 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2941 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3001 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3061 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3121 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3181 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3241 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3301 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3361 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3421 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3481 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3541 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3601 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3661 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3721 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3781 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3841 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3901 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3961 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     4021 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     4081 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     4141 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     4201 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     4261 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     4321 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     4381 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     4441 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     4501 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4561 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4621 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     4681 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     4741 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     4801 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     4861 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     4921 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     4981 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     5041 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     5101 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     5161 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     5221 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     5281 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     5341 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     5401 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     5461 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     5521 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     5581 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     5641 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     5701 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     5761 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     5821 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     5881 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     5941 ccgaggggac ccatgcaggg aggtggtccc tgcagttacg ccaatgataa cccccgccag
     6001 aaaaatctta gtagccttcc ctttttgttt tccgtgcccc aactcggcgg attgactcgg
     6061 ccccttccgg aaacacccga atcaacttct agtcaaatta ttgttcacgc cgcaatgacc
     6121 cacccctggc ccgcgtctgt ggaactgacc cctggtctac aggagagttc gctgctgaaa
     6181 gtggtcccaa aggggtacta gtttttaagc tcccaactcc ccctccccca gcgtctggag
     6241 gattccacac cctcgcaccg caggggcgag gaagtgggcg gagtccggtt ttggcgccag
     6301 ccgctgaggc tgccaagcag aaaagccacc gctgaggaga ctccggtcac tgtcctcgcc
     6361 ccgcctcccc cttccctccc cttggggacc accgggcgcc acgccgcgaa cggtaagtgc
     6421 cgcggtcgtc ggcgcctccg ccctccccgt agggccccaa ttcccagcgg gcgcggcgcg
     6481 cggccccgcg cgcagctccc ggctccctcc cccttcggat gtggcttgag ctgtaggcgc
     6541 ggagtcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     6601 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
     6661 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
     6721 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
     6781 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     6841 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     6901 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     6961 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     7021 tcctccgaca gactgagtcg cccggggctt ttttggagga ctaggctttt gcaaaaagct
     7081 ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     7141 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     7201 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     7261 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     7321 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     7381 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     7441 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     7501 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     7561 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     7621 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     7681 gcatgacccg caagcccggt gccagtggat ccggagccac gaacttctct ctgttaaagc
     7741 aagcaggaga tgttgaagaa aaccccgggc ctatgaaacg gacagcggac ggtagcgagt
     7801 ttgagagtcc gaagaaaaag aggaaagtag cttctaactt tactcagttc gttctcgtcg
     7861 acaatggcgg aactggcgac gtgactgtcg ccccaagcaa cttcgctaac gggatcgctg
     7921 aatggatcag ctctaactcg cgttcacagg cttacaaagt aacctgtagc gttcgtcaga
     7981 gctctgcgca gaatcgcaaa tacaccatca aagtcgaggt gcctaaaggc gcctggcgtt
     8041 cgtacttaaa tatggaacta accattccaa ttttcgccac gaattccgac tgcgagctta
     8101 ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc gattccctca gcaatcgcag
     8161 caaactccgg catctacgag gcctcgggag gtggttcggg tggctctgga tcaggatccg
     8221 ctgtacagcc ttcagggcag atcagcaacc aggccctggc tctggcccct agctccgctc
     8281 cagtgctggc ccagactatg gtgccctcta gtgctatggt gcctctggcc cagccacctg
     8341 ctccagcccc tgtgctgacc ccaggaccac cccagtcact gagcgctcca gtgcccaagt
     8401 ctacacaggc cggcgagggg actctgagtg aagctctgct gcacctgcag ttcgacgctg
     8461 atgaggacct gggagctctg ctggggaaca gcaccgatcc cggagtgttc acagatctgg
     8521 cctccgtgga caactctgag tttcagcagc tgctgaatca gggcgtgtcc atgtctcata
     8581 gtacagccga accaatgctg atggagtacc ccgaagccat tacccggctg gtgaccggca
     8641 gccagcggcc ccccgacccc gctccaactc ccctgggaac cagcggcctg cctaatgggc
     8701 tgtccggaga tgaagacttc tcaagcatcg ctgatatgga ctttagtgcc ctgctgtcac
     8761 agatttcctc tagtgggcag ggaggaggtg gaagcggctt cagcgtggac accagtgccc
     8821 tgctggacct gttcagcccc tcggtgaccg tgcccgacat gagcctgcct gaccttgaca
     8881 gcagcctggc cagtatccaa gagctcctgt ctccccagga gccccccagg cctcccgagg
     8941 cagagaacag cagcccggat tcagggaagc agctggtgca ctacacagcg cagccgctgt
     9001 tcctgctgga ccccggctcc gtggacaccg ggagcaacga cctgccggtg ctgtttgagc
     9061 tgggagaggg ctcctacttc tccgaagggg acggcttcgc cgaggacccc accatctccc
     9121 tgctgacagg ctcggagcct cccaaagcca aggaccccac tgtctccgct agcggcacct
     9181 agg
//
