LOCUS       sequence-431549-       10450 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Encodes SARS-CoV-2 variant XBB Spike for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 XBB
FEATURES             Location/Qualifiers
     source          1..10450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3828..4416
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3881..3901)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4299..4310)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4469..4590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4480..4499)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4534..4553
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4705..4751
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4781..4917
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4962..5033
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5078..5274
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5125..5260
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5288..6082
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5342..5361)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5952..5971
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6093..6317
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6513..7101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6593..6612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7847..7915,7916..8707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8065..8084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8720..8856
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9008..9079
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9084..9130)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9148..9196
                     /note="synthetic polyadenylation signal"
     enhancer        9277..9580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9582..9784
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9735..9755
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9918..10393
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 catgtttgtg ttcctcgttc tcctgcccct cgtttcttcc caatgcgtga acctcataac
       61 tagaacgcag tcttacacga actcttttac tcgtggagta tactatcccg ataaagtctt
      121 taggtccagt gtccttcatt ctactcaaga ccttttcctg ccattcttct caaatgtcac
      181 ctggttccat gctatccacg tttctggcac caacggaact aaacgatttg ataatcccgc
      241 cttgccattt aacgatggcg tatactttgc ctcaacggag aagtccaaca ttatccgggg
      301 atggatattt ggcaccaccc tggactccaa gacacagtct ctcctcattg tgaacaacgc
      361 caccaatgtc gtcatcaaag tctgtgaatt tcaattttgt aacgatccct ttctcgatgt
      421 ttatcagaag aataacaagt cttggatgga gtcagagttc cgcgtttaca gttccgctaa
      481 caactgtact tttgagtacg tttcccaacc atttctcatg gatcttgaag ggaaggaagg
      541 caacttcaag aatctccgcg aatttgtgtt taagaatata gacggctact tcaaaattta
      601 tagcaagcat accccgataa atctggagcg ggatctccct caaggcttct ccgcgttgga
      661 acccctcgtg gacttgccga ttggcatcaa catcacccgg ttccaaaccc ttcttgccct
      721 tcatcgaagc tacctcacgc ctgtcgacag tagtagtggt tggacagcgg gcgcagcagc
      781 ctactatgtg ggatatttgc aaccacgcac ctttcttctc aaatataacg aaaacggaac
      841 aattaccgat gccgtagact gtgccctcga tcccctgtcc gaaactaaat gcacactcaa
      901 gtccttcaca gttgagaaag gcatatacca aaccagtaac ttccgcgtcc aacccaccga
      961 atccattgtt cgattcccta acatcaccaa tttgtgtcct ttccatgaag tatttaatgc
     1021 cactacattt gcatcagttt atgcctggaa tcgcaagcga atatcaaatt gtgttgcaga
     1081 ttatagtgtc atctataatt tcgcaccatt cttcgctttt aagtgttatg gggtcagccc
     1141 tacgaaattg aatgacttgt gttttaccaa cgtttacgca gattctttcg tcatacgcgg
     1201 caacgaagtc agccagattg caccggggca aaccggaaat atagcagact ataattataa
     1261 gttgccggat gacttcaccg gctgcgttat agcatggaac tccaataagc tcgattcaaa
     1321 acccagcggc aactataatt atctttaccg cctgtttcgc aagtcaaagc tgaagccctt
     1381 tgaacgtgac atctccaccg aaatttatca agctggcaac aaaccatgta atggagtggc
     1441 agggtctaac tgttattctc cccttcaatc atacggattt cgaccaacat atggggtggg
     1501 ccatcaacca tatcgggtgg tagtcttgag ctttgagctg ctccacgcac cggctacagt
     1561 ttgcggaccg aagaaaagca ctaacctggt gaagaataaa tgtgtgaact tcaactttaa
     1621 tgggctcacc ggaacagggg tgctgacaga atcaaacaag aaatttctcc cttttcaaca
     1681 atttggtaga gacattgccg ataccacaga tgccgtccgc gatccgcaaa cactggaaat
     1741 cctcgatata acaccctgta gcttcggcgg ggtcagcgtg ataactcccg gaacgaacac
     1801 atccaaccaa gtggctgtcc tgtatcaagg tgtcaactgt accgaagtcc ctgtggcgat
     1861 acacgccgac caactgacac ccacctggag agtctactca acaggttcca atgtctttca
     1921 aactagagcg ggttgtctca ttggcgctga gtatgtcaat aattcatacg aatgtgatat
     1981 tcctattggg gcaggaatct gtgcgtctta tcaaactcaa acgaaatcac accggcgcgc
     2041 tcgctcagta gccagccagt ccataatcgc ctataccatg agtctcgggg ctgagaactc
     2101 cgtggcttat tcaaacaatt ccatcgcaat cccaactaac tttaccatta gcgttaccac
     2161 ggaaatcttg cccgtcagca tgactaagac gagcgtagac tgtacgatgt atatctgtgg
     2221 ggattccacc gagtgtagca acctcttgct tcaatacggg tctttctgta ctcaactcaa
     2281 acgagcattg accggaattg cggtcgaaca agacaagaat acccaagagg tgttcgcaca
     2341 agtaaagcaa atatacaaaa cgccgccaat caaatatttt ggcgggttta attttagcca
     2401 aatactgcca gacccatcta agccttcaaa acgcagcttt atcgaggatc tgctgttcaa
     2461 caaagttacg ttggctgacg cgggtttcat taagcagtat ggggattgtt tgggagacat
     2521 tgcggcccgc gaccttatct gtgctcaaaa attcaatggg ttgacagttc tgccccctct
     2581 gctcactgat gagatgattg cacagtatac atcagcattg ttggcaggaa ccattacatc
     2641 cggctggact tttggcgcgg gtgcggccct tcaaatacct ttcgctatgc aaatggctta
     2701 cagattcaat gggattggtg tcacgcaaaa tgtattgtat gaaaatcaga agctgatcgc
     2761 taatcaattt aacagcgcga ttggcaagat ccaagattca ctgtcaagca cagcgtccgc
     2821 cctcggcaaa ctccaagatg ttgtgaatca taacgcccag gccctgaata ccctcgtgaa
     2881 gcaactgagt tctaaattcg gcgcaatctc cagcgtcctc aacgacatat tgtccagact
     2941 tgataaggtg gaagccgagg tgcaaattga tcggctcatc acaggcaggc tccagtcact
     3001 ccaaacttat gtgacacaac agttgatccg ggctgctgag atacgagcaa gcgccaacct
     3061 ggccgccact aaaatgtcag agtgcgtgct cgggcagtcc aaacgggttg acttttgtgg
     3121 caagggctat cacttgatgt ccttccccca atccgcccca catggagtgg tctttctcca
     3181 cgtcacttat gtccctgctc aagagaagaa ctttacgaca gctccagcaa tctgtcacga
     3241 tggcaaagcg cactttccca gggaaggcgt tttcgtcagt aatggcacgc attggtttgt
     3301 gacccaaagg aatttctatg aaccccaaat cataaccact gacaatacat ttgtctcagg
     3361 caactgtgat gtggtcattg ggatcgtgaa caacacggta tatgatcccc tgcaacccga
     3421 gttggatagc ttcaaagagg aattggataa atactttaag aatcatactt cacccgatgt
     3481 tgatctcggg gacatatcag gaataaatgc aagtgtcgtc aacattcaga aagagattga
     3541 tcgccttaat gaggtcgcca agaacttgaa tgaatctttg atcgacttgc aagaactggg
     3601 caaatacgaa caatatatta aatggccctg gtacatttgg ttgggcttta tcgccggact
     3661 tatagctatc gttatggtca caattatgtt gtgctgtatg actagctgtt gttcctgctt
     3721 gaaaggatgt tgcagttgcg gatcttgttg taaataaagg atcctctaga agttgtctcc
     3781 tcctgcactg actgactgat acaatcgatt tctggatccg caggcctaat caacctctgg
     3841 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3901 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3961 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4021 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4081 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4141 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4201 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     4261 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4321 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4381 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ctagcttgac tgactgagat
     4441 acagcgtacc ttcagctcac agacatgata agatacattg atgagtttgg acaaaccaca
     4501 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4561 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4621 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     4681 attggcccat ctctatcggt atcgtagcat aaccccttgg ggcctctaaa cgggtcttga
     4741 ggggtttttt gtgcccctcg ggccggattg ctatctaccg gcattggcgc agaaaaaaat
     4801 gcctgatgcg acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg
     4861 gcttccccaa cttgcccact tccatacgtg tcctccttac cagaaattta tccttaaggt
     4921 cgtcagctat cctgcaggcg atctctcgat ttcgatcaag acattccttt aatggtcttt
     4981 tctggacacc actaggggtc agaagtagtt catcaaactt tcttccctcc ctaatctcat
     5041 tggttacctt gggctatcga aacttaatta agcgatctgc atctcaatta gtcagcaacc
     5101 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5161 ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5221 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaggaggt
     5281 agccaacatg attgaacaag atggattgca cgcaggttct cccgccgctt gggtggagag
     5341 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5401 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5461 tgaactccag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5521 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5581 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5641 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5701 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5761 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcggat
     5821 gcccgacggc gaggatctcg tcgtgaccca cggcgatgcc tgcttgccga atatcatggt
     5881 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5941 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6001 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6061 ccttcttgac gagttcttct agtatgtaag ccctgtgcct tctagttgcc agccatctgt
     6121 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6181 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6241 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6301 tgcggtgggc tctatggtta attaaccagt caagtcagct acttggcgag atcgacttgt
     6361 ctgggtttcg actacgctca gaattgcgtc agtcaagttc gatctggtcc ttgctattgc
     6421 acccgttctc cgattacgag tttcatttaa atcatgtgag caaaaggcca gcaaaaggcc
     6481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7141 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7201 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7261 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7321 cgttcatcca tagttgcatt taaatttccg aactctccaa ggccctcgtc ggaaaatctt
     7381 caaacctttc gtccgatcca tcttgcaggc tacctctcga acgaactatc gcaagtctct
     7441 tggccggcct tgcgccttgg ctattgcttg gcagcgccta tcgccaggta ttactccaat
     7501 cccgaatatc cgagatcggg atcacccgag agaagttcaa cctacatcct caatcccgat
     7561 ctatccgaga tccgaggaat atcgaaatcg gggcgcgcct ggtgtaccga gaacgatcct
     7621 ctcagtgcga gtctcgacga tccatatcgt tgcttggcag tcagccagtc ggaatccagc
     7681 ttgggaccca ggaagtccaa tcgtcagata ttgtactcaa gcctggtcac ggcagcgtac
     7741 cgatctgttt aaacctagat attgatagtc tgatcggtca acgtataatc gagtcctagc
     7801 ttttgcaaac atctatcaag agacaggatc agcaggaggc tttcgcatga gtattcaaca
     7861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcgcgag tgggttacat
     7981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgctttcc
     8041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtattcacc
     8161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8221 aaccatgagt gataacactg cggccaactt acttctgaca acgattggag gaccgaagga
     8281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8401 aacaaccttg cgtaaactat taactggcga actacttact ctagcttccc ggcaacagtt
     8461 gatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8701 ttggtaaccg attctaggtg cattggcgca gaaaaaaatg cctgatgcga cgctgcgcgt
     8761 cttatactcc cacatatgcc agattcagca acggatacgg cttccccaac ttgcccactt
     8821 ccatacgtgt cctccttacc agaaatttat ccttaagatc ccgaatcgtt taaactcgac
     8881 tctggctcta tcgaatctcc gtcgtttcga gcttacgcga acagccgtgg cgctcatttg
     8941 ctcgtcgggc atcgaatctc gtcagctatc gtcagcttac ctttttggca gcgatcgcgg
     9001 ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt ggtcataaca
     9061 gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga ggccccaagg
     9121 ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc catcttcgat
     9181 ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt aatcaattac
     9241 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     9301 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     9361 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     9421 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     9481 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     9541 ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt tttggcagta
     9601 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     9661 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     9721 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     9781 agctggttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     9841 tagaagacac cgggaccgat ccagcctccc ctcgaagctt acatgtggta ccgagctcgg
     9901 atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc ccttcttttc
     9961 tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata ttgaccaaat
    10021 cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata tacttttttg
    10081 tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg
    10141 tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat
    10201 agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc
    10261 atattgctaa tagcagctac aatccagcta ccattctgct tttattttat ggttgggata
    10321 aggctggatt attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat
    10381 cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa
    10441 aggcggccgc
//
