LOCUS       sequence-431786-       11969 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of a cytosolic-localized split mNeonGreen 
            variant that enables efficient complementation when the missing beta
            strand is present. mKate2 is present as a transduction control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11969)
  TITLE     Hartl Lab plasmids
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF1a mNeonGreen-3K-B1-10-IRES-mKate2-2A-PuroR
FEATURES             Location/Qualifiers
     source          1..11969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        130..1308
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          361..1299
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1256..1276
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1375..1416
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      1684..1693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2071..2123
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    join(2125..2699,2700..2702,2703..2711)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     2519..2538
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2712..3407
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             3417..3473
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3486..4079
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="EYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVT
                     ELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQ
                     QQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAP
                     RNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     3976..3996
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4104..4692
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4157..4177)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4575..4586)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4695..4711)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4696..4712)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5217..5397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5423..5440)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5429..5653
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5699..6127
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5786..5805)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5996..6017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6136..6156)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6141..6470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6321..6456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6383..6402
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6518..6565
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6584..6958
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7088..7209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7125..7144)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7179..7198
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7258..7274)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7258..7274)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7271..7293)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7282..7298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7306..7312,7313..7330,7331..7336))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7351..7372
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7489..7506)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7660..8248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7740..7759)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8419..9210,9211..9279))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9042..9061
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9280..9384)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9459..9478)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9650..10029
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10031..10229
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10184..10204
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10247..10427
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10474..10599
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11092..11325
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11510..11554
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11852..11969
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgggtttatt acagggacag cagagatcca gtttggttaa ttagctagct gcaaagatgg
       61 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
      121 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
      181 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
      241 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
      301 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
      361 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
      421 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
      481 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
      541 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
      601 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
      661 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
      721 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
      781 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
      841 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
      901 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
      961 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     1021 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     1081 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     1141 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     1201 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     1261 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgacg tacgctagag
     1321 gatcgaaccc ttgccaccat gttggaactg ctggaagatc tgaccctggg tagtggcaag
     1381 cccatcccca accccctgct gggcctggac agcaccggcg gaggtggatc cgtgagcaag
     1441 ggcgaggagg ataacatggc ctctctccca gcgacacatg agttacacat ctttggctcc
     1501 atcaacgatg tggactttga catggtgggt cagggcaccg gcaatccaaa tgaaggttat
     1561 gaggagttaa acctgaagtc caccaagggt gacctccagt tctccccctg gattctggtc
     1621 cctcatatcg ggtatggctt ccatcagtac ctgccctacc ctgacgggat gtcgcctttc
     1681 caggccgcca tggtagatgg ctccggctac caagtccatc gcacaatgca gtttgaagat
     1741 ggtgcctccc ttactgttaa ctaccgctac acctacgagg gaagccacat caaaggagag
     1801 gcccaggtga ttgggactgg tttccctgct gacggtcctg tgatgaccaa caccctgacc
     1861 gctgcggact ggtgcatgtc gaagatgact taccccaacg acaaaaccat catcagtacc
     1921 tttaagtgga gttacattac tgtgaatggc aagcgctacc ggagcactgc gcggaccacc
     1981 tacacctttg ccaagccaat ggcggctaac tatctgaaga accagccgat gtacgtgttc
     2041 cgtaagacgg agctcaagca ctccatgtaa ctcgagctca agcttcgaat tctgcagtcg
     2101 acggtaccgc gggcccggga tccgcccctc tccctccccc ccccctaatg ttactggccg
     2161 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     2221 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     2281 gggtctttcc cctctcacca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     2341 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     2401 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     2461 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     2521 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     2581 gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa
     2641 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     2701 tggccacaac catggtgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
     2761 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg
     2821 agggcaccca gaccatgaga atcaaggcgg tcgagggcgg ccctctcccc ttcgccttcg
     2881 acatcctggc taccagcttc atgtacggca gcaaaacctt catcaaccac acccagggca
     2941 tccccgactt ctttaagcag tccttccccg agggcttcac atgggagaga gtcaccacat
     3001 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     3061 tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga
     3121 aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc cgctgacggc ggcctggaag
     3181 gcagagccga catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttgaaga
     3241 ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg
     3301 acagaagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg
     3361 tggctgtggc cagatactgc gacctcccta gcaaactggg gcacagagga tctggtgcca
     3421 cgaatttctc gctactcaag caggccggtg atgtcgagga aaaccctggt cctatggtgt
     3481 ctagagagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     3541 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     3601 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     3661 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     3721 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     3781 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     3841 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     3901 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     3961 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     4021 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag
     4081 aattcgatat caagcttatc ggtaatcaac ctctggatta caaaatttgt gaaagattga
     4141 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4201 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4261 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4321 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4381 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4441 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     4501 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4561 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4621 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4681 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     4741 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     4801 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     4861 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     4921 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     4981 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     5041 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     5101 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     5161 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     5221 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5281 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5341 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     5401 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     5461 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     5521 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     5581 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     5641 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     5701 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5761 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5821 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5881 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5941 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6001 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6061 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6121 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     6421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     6481 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     6541 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     6601 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     6661 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     6721 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     6781 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     6841 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     6901 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     6961 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7021 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7081 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7141 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7201 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7261 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7321 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7381 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7441 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7501 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7561 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7621 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7681 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7741 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7801 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7861 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7921 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7981 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8041 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8101 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8161 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8221 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8281 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8341 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8401 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8461 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8521 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8581 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8641 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8701 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8761 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8821 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8881 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8941 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9001 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9061 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9121 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9181 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9241 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9301 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9361 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     9421 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     9481 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     9541 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     9601 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     9661 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     9721 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     9781 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     9841 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     9901 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     9961 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    10021 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
    10081 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
    10141 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
    10201 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
    10261 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10321 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10381 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
    10441 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
    10501 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
    10561 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
    10621 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
    10681 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
    10741 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    10801 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
    10861 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
    10921 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    10981 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    11041 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    11101 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    11161 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    11221 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    11281 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    11341 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    11401 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    11461 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    11521 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    11581 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    11641 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    11701 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    11761 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
    11821 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    11881 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    11941 aaaaacaaat tacaaaaatt caaaatttt
//
