LOCUS       sequence-431879-        7503 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express Pex15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 7503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB717
FEATURES             Location/Qualifiers
     source          1..7503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1871..2058
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1926..1950)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2534..2941
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2942..4036
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(5067..5882)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5067..5882)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5790..5809
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5874..5893
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6107..6695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6596..6615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatgg ctgcaagtga
      721 gataatgaac aatctgccta tgcattccct cgattcttct ctaagggatt tacttaacga
      781 tgacttattc attgaaagtg atgaatcgac caagtcggta aatgatcaga ggagtgaagt
      841 atttcaagaa tgtgtgaacc ttttcataaa aagagatatc aaggattgcc tagaaaaaat
      901 gtctgaggtt ggatttattg atattaccgt tttcaagtcc aaccctatga tattagattt
      961 atttgtcagc gcctgtgata ttatgcccag ctttaccaaa ttaggtttaa cactgcagag
     1021 tgagattttg aatattttca cgttagatac ccctcaatgt attgagacaa gaaaaattat
     1081 tctcggtgac ctcagtaaac ttttggtcat caataaattc tttcgatgct gtatcaaggt
     1141 tattcagttt aacttaactg atcacaccga acaagaagaa aaaactctgg agcttgaatc
     1201 cataatgagt gatttcattt tcgtatacat aacgaaaatg aggacaacta tagatgtagt
     1261 tggcttacaa gaattaatcg agatattcat ctttcaggtg aaggtaaaac tgcatcataa
     1321 aaaaccctca ccgaatatgt attgggcgct ttgcaaaaca ttacctaagt tatctccaac
     1381 cctaaagggc ctttacttat ctaaagatgt ttccatagag gatgctattc tgaactcaat
     1441 agataataaa atacaaaaag ataaggcgaa gtctaaaggt aaacaaagag gagtgaaaca
     1501 gaaaatacac cattttcacg agcctatgtt acacaatagt agtgaagaac aggttaaggt
     1561 tgaggatgcg tttaatcaac gcacctctac tgatagtaga ctccagagta ctggaacggc
     1621 gccccgtaaa aaaaacaatg atattactgt tctggctggt tcattttggg ctgtcctgaa
     1681 gcatcatttc acaagaagtg tgctgaacaa aaacggactt ctgctcacag gtctgctgct
     1741 actcttatgt ttgaaaaaat ataagtcatt gatggcaatt ttcaaacatg ttccagcggc
     1801 ctttcacact gtataccccc agattgtagg gttgctaaaa cttctagcga gtataggatc
     1861 ctaactcgag gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     1921 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
     1981 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
     2041 tgaccacacc tctaccggca tgccgagcaa atgcctgcaa atcgctcccc atttcgctgg
     2101 aaatctgctc gtcagtggtg ctcacactga cgaatcatgt acagatcata ccgatgactg
     2161 cctggcgact cacaactaag caagacagcc ggaaccagcg ccggcgaaca ccactgcata
     2221 tatggcatat cacaacagtc cacgtctcaa gcagttacag agatgttacg aaccactagt
     2281 gcactgcagt acatcgagga gaacttctag tatatctaca tacctaatat tattgcctta
     2341 ttaaaaatgg aatcccaaca attacatcaa aatccacatt ctcttcaaaa tcaattgtcc
     2401 tgtacttcct tgttcatgtg tgttcaaaaa cgttatattt ataggataat tatactctat
     2461 ttctcaacaa gtaattggtt gtttggccga gcggtctaag gcgcctgatt caagaaatat
     2521 cttgaccgca gttaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc
     2581 ttagcaacca ttattttttt cctcaacata acgagaacac acaggggcgc tatcgcacag
     2641 aatcaaattc gatgactgga aattttttgt taatttcaga ggtcgcctga cgcatatacc
     2701 tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac cggcttttca
     2761 tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga agttgacaat
     2821 attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt actttctaac
     2881 ttttcttacc ttttacattt cagcaatata tatatatata tttcaaggat ataccattct
     2941 aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac
     3001 agccgaagcc attaaggttc ttaaagctat ttctgatgtt cgttccaatg tcaagttcga
     3061 tttcgaaaat catttaattg gtggtgctgc tatcgatgct acaggtgttc cacttccaga
     3121 tgaggcgctg gaagcctcca agaaggctga tgccgttttg ttaggtgctg tgggtggtcc
     3181 taaatgggga accggtagtg ttagacctga acaaggttta ctaaaaatcc gtaaagaact
     3241 tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc gactctcttt tagacttatc
     3301 tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag aattagtggg
     3361 aggtatttac tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga
     3421 acaatacacc gttccagaag tgcaaagaat cacaagaatg gccgctttca tggccctaca
     3481 acatgagcca ccattgccta tttggtcctt ggataaagct aatgttttgg cctcttcaag
     3541 attatggaga aaaactgtgg aggaaaccat caagaacgaa tttcctacat tgaaggttca
     3601 acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg
     3661 tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc
     3721 aggttccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc
     3781 atttggtttg tacgaaccat gccacggttc tgctccagat ttgccaaaga ataaggtcaa
     3841 ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga
     3901 agaaggtaag gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg
     3961 tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa
     4021 gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa
     4081 tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg
     4141 aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga
     4201 atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt
     4261 aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca
     4321 tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa aaaaatacaa
     4381 caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat accttcttga
     4441 accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga aacggcctta
     4501 acgacgtagt cgagagtact cgtatcgcat gtcggtgcga cacgaaatta caaaatggaa
     4561 tatgttcata gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga
     4621 aaaaggagga tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa
     4681 ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt
     4741 taggtgtaac agaaaatcat gaaactatga ttcctaattt atatattgga ggattttctc
     4801 taaaaaaaaa aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt
     4861 taagtcaata ccttcttgaa ccatttccca taatggtgaa agttccctca agaattttac
     4921 tctgtcagaa acggccttaa cgacgtagtc gacctcctct tcagtactaa atctaccaat
     4981 accaaatctg atggaagaat gggctaatgc atcatcctta cccagcgccg agcggccgcg
     5041 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc
     5101 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     5161 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     5221 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     5281 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt
     5341 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     5401 accaaaccgt tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta
     5461 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     5521 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     5581 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     5641 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     5701 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     5761 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     5821 gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc
     5881 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
     5941 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata
     6001 aatcgaactt ttgctgagtt gaaggatcag tcatgaccaa aatcccttaa cgtgagtttt
     6061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6241 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6481 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6721 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6781 ctgtggataa ccgtgcggcc gccaataccc agaagaacag taaaataaag caaggtacgt
     6841 gaaattaata tttttaaatg gttctaaccg atgccgaaga actgcgcagt ccggttataa
     6901 cgtctgacat gtcctttttt gatttggaat ccaaccactc aagtgactct gttcatttac
     6961 tttgcgaaaa atatacccac aaattgccca tcgaaagtga atcgcaaacc accttcagac
     7021 tggcaccgac aaagcaaaga ttatacagac agagtacttt atacgtaccg ttaagtctca
     7081 agcaaagggt tttcttattt actgaacggg taaagagtat ctgggccggc ttgccaagat
     7141 gcaaaccgaa taagtatttc aaagttgcat ttgccttagc cgtcctgaca ccattggcta
     7201 tttggatatt ttatattgac tttcgtgtac attgatcaca tcgactgttc tattggcaaa
     7261 tgaaccacgg gcattgacta tttttcaggt tactactata tattatcatc acgggcaagg
     7321 attgtaccct gaattcgcat ctagactgat gagacgtggt agagccacaa acagccggta
     7381 caagcaacga tctccaggac catctgaatc atgcgcggat gacacgaact cacgacggcg
     7441 atcacagaca ttaacccaca gtacagacac tgcgacaacg tggcaattcg tcgcaataca
     7501 acg
//
