LOCUS       sequence-431890-       17376 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express Pex15, Pex22, Pex4, Pex1, and Pex6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17376)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 17376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1402
FEATURES             Location/Qualifiers
     source          1..17376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3491..3678
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3546..3570)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             13649..14452
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(15094..15909)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(15094..15909)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     15817..15836
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     15901..15920
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      16134..16722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16623..16642
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgactag agatctatgt gaggatccta actcgagatc
       61 gagctgggta gactacccat gagtcacaat gaacgacgat ggcttcttat ctcacttcaa
      121 tagtactttc caccggttat acttccggct tttccctatt aatacaagct acaatttcaa
      181 tgggtggcaa ataatgtgta gaatagaaaa taagccgaca gggtaataaa gaaaattttt
      241 agaaaaaaaa ggttagatgg cttatttaag ttacaggcta gcgaaaaaag gaacttcagg
      301 gcaagtaaag tgtttgattg ggcactagca tggcttataa aggcgagcaa ttgtcgaaac
      361 taattaatgt tgtacggact attgctgtca tctcgtggta aatgcgtgtt ccaggtcgaa
      421 tactacttgc acacaggcga gcggggcccc ataaaagtgt tgccgatttg ttaagttgtc
      481 ttttcggttt ttctactctg ttattcctta cttccctttt taagaactct ttttatcctt
      541 catttaggat cttgcacgtt tccgcctcat cacttgaatt aaaacatgtc tctgtcagta
      601 aaccttggcg tttctattgt tcttcatagt tcaactttta ttattacccg ccctgcgcgt
      661 ttacattttt ccagcaacag ccagcgaaaa attagaaaat ctggttgttg acacctcaag
      721 aacaagggca attagcctca gcgtcgaata tagatcatat tagaatacct atagctccat
      781 caaaagaaat acacaagatc tatggctgca agtgagataa tgaacaatct gcctatgcat
      841 tccctcgatt cttctctaag ggatttactt aacgatgact tattcattga aagtgatgaa
      901 tcgaccaagt cggtaaatga tcagaggagt gaagtatttc aagaatgtgt gaaccttttc
      961 ataaaaagag atatcaagga ttgcctagaa aaaatgtctg aggttggatt tattgatatt
     1021 accgttttca agtccaaccc tatgatatta gatttatttg tcagcgcctg tgatattatg
     1081 cccagcttta ccaaattagg tttaacactg cagagtgaga ttttgaatat tttcacgtta
     1141 gatacccctc aatgtattga gacaagaaaa attattctcg gtgacctcag taaacttttg
     1201 gtcatcaata aattctttcg atgctgtatc aaggttattc agtttaactt aactgatcac
     1261 accgaacaag aagaaaaaac tctggagctt gaatccataa tgagtgattt cattttcgta
     1321 tacataacga aaatgaggac aactatagat gtagttggct tacaagaatt aatcgagata
     1381 ttcatctttc aggtgaaggt aaaactgcat cataaaaaac cctcaccgaa tatgtattgg
     1441 gcgctttgca aaacattacc taagttatct ccaaccctaa agggccttta cttatctaaa
     1501 gatgtttcca tagaggatgc tattctgaac tcaatagata ataaaataca aaaagataag
     1561 gcgaagtcta aaggtaaaca aagaggagtg aaacagaaaa tacaccattt tcacgagcct
     1621 atgttacaca atagtagtga agaacaggtt aaggttgagg atgcgtttaa tcaacgcacc
     1681 tctactgata gtagactcca gagtactgga acggcgcccc gtaaaaaaaa caatgatatt
     1741 actgttctgg ctggttcatt ttgggctgtc ctgaagcatc atttcacaag aagtgtgctg
     1801 aacaaaaacg gacttctgct cacaggtctg ctgctactct tatgtttgaa aaaatataag
     1861 tcattgatgg caattttcaa acatgttcca gcggcctttc acactgtata cccccagatt
     1921 gtagggttgc taaaacttct agcgagtata tgaggatcct aactcgagag tgcttttaac
     1981 taagaattat tagtcttttc tgcttatttt ttcatcatag tttagaacac tttatattaa
     2041 cgaatagttt atgaatctat ttaggtttaa aaattgatac agttttataa gttacttttt
     2101 caaagactcg tgctgtctat tgcataatgc actggaaggg gaaaaaaaag gtgcacacgc
     2161 gtggcttttt cttgaatttg cagtttgaaa aatgctgaag tgaataaagc tccacacagt
     2221 cgaacgttcg tgctttgtga taaagtgttt cacgtcatcc gacatgactt cgtagttatg
     2281 gactgaactg tgtggtgagg ttccatgatt tcttaggtcc agcagataca tgtctcttcc
     2341 caatttcttg ttaaggttac ggccaatgct tcggttgttg agcttgttac cgaataagcc
     2401 gtgaagtatg ataataggtg gtcttggctt cccttcatcc ccagttttta ctgcatctct
     2461 cttgattatg tcatatgaaa ggtccagtgg gacttgcttt tgttgcagca cctttgctaa
     2521 tgaatgaaag gcacatagtg actgcttaaa aatgcaggaa cttaaattat tccgaatggt
     2581 attttgtctc acatatattg tcccatactg tgccaagatc ccggctttac ccagtatcat
     2641 cattgtaccg ttaccaattc tcctcgtata tcacggttag tttttaaacc tcggggtgac
     2701 gtttactatt ggcgtactaa tatattctta ttttcttttc ttttttgttg gcagtttcaa
     2761 gcaacacatg tactggataa ccaacccccg cacgctcttg gaaaaaattg agaaggcatc
     2821 ggacacttgc tgatgagtat ttcgaaaaat tccatgaaga tgaggccaag attgtttgga
     2881 agagattgaa aagaagaaga agaaaaaaag ataaaagcaa atcaaaagat ctatgccacc
     2941 accatcaaga agtagaataa acaaaacaag aacattagga atagtgggta cagctatagc
     3001 agtgttggtc acgtcctact atatatatca aaaggtgaca agtgcaaagg aagataatgg
     3061 ggcacgacct ccagagggtg attcagtaaa agagaacaaa aaggcaagga agagcaaatg
     3121 tattataatg agcaagtcga tacaaggact gcccataaag tgggaggagt acgccgctga
     3181 tgaagtggtt ttgctggtac ctacgagcca cactgatgga tcaatgaaac aagccattgg
     3241 ggatgccttt cgcaagacga aaaacgaaca caaaatcata tattgcgata gcatggatgg
     3301 attatggtca tgtgtaagac ggctaggtaa atttcagtgc atattgaact ccagggactt
     3361 cacaagtagt ggtggtagcg atgcagcagt cgttcctgaa gatataggca ggtttgtcaa
     3421 atttgttgtt gatagcgatg tagaggatgt gctgattgac actttatgca attaaggatc
     3481 ctaactcgag gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     3541 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
     3601 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
     3661 tgaccacacc tctaccggca tgccgagcaa atgcctgcaa atcgctcccc atttcgctgc
     3721 cctagggaca ctgacacaat tccaaacgtt tgagaatgac cttccacgag ctaaattgaa
     3781 agggaagaat ttattagttg aactcaagaa agaagaggat gacgtgggaa atggcataga
     3841 atcccttact aaatcgaaca ctaaactgaa ctccatgctg gcgaacgaag gtaagataca
     3901 caaagctagt ttccagaaaa gtgtaaaatt taaactacct gataatatag tgactgaaga
     3961 aaccgtggaa cttaaagaaa taaaggactt gctactacaa atgttgagat gacagcgaga
     4021 gattgaatca agattatcca atatcgaact tcaactcacg gaaataccga aacataagta
     4081 atcatatccc ttctcacatt ttttacacag gaagtaagca agttatgtta tatttccgac
     4141 actataatta attcttagca gttaaaggtg ctttgtctat attacattta catacagctt
     4201 gagtgatcct gaccggatat agggtcctat tttcttacgt gaacggcttt tcttcttgtt
     4261 cccgatggcc ttcatgtgaa aaagcactcc tcgggaggcg gaaaaatatc aaaagtacgg
     4321 ggcgaagttt ataatgaaga tttatcgata taaattttgg ttatttcagg agaacaagaa
     4381 agctctttac actaaaatta tcagagaaga agctgatata ttagccctaa ggagtacacc
     4441 aaaacacaag atctatgcca aacttctgga ttcttgagaa tcgtaggagc tatacgtctg
     4501 atacatgtat gtcgaggatc gtgaaggagt acaaagtcat actgaaaaca ttggccagtg
     4561 atgaccccat agcgaacccc taccggggga taatcgagtc acttaacccg attgacgaaa
     4621 ccgacttgag caagtgggaa gctataattt ctgggccgtc agacacacct tatgaaaatc
     4681 atcagtttcg aatactaata gaagtcccta gttcctaccc catgaatcca ccaaagataa
     4741 gttttatgca gaacaatatt ttgcactgca atgttaaatc agccacagga gaaatatgtc
     4801 tgaacatcct taaaccagag gagtggactc ctgtatggga tttgttacac tgtgttcatg
     4861 ctgtttggag actcctaagg gaacccgttt gtgattctcc acttgacgta gacattggta
     4921 atatcatccg ctgtggcgat atgagtgcct atcaggggat tgtaaagtac ttcctagcag
     4981 aaagagagcg gatcaacaac cattgaggat cctaactcga ggccaattgg tgcggcaatt
     5041 gataataacg aaaatgtctt ttaatgatct gggtataatg aggaattttc cgaacgtttt
     5101 tactttatat atatatatac atgtaacata tattctatac gctatagaga aaggaaattt
     5161 ttcaattaaa aaaaaaatag agaaagagtt tcacttcttg attatcgcta acactaatgg
     5221 ttgaagtact gctactttaa ttttatgctg gcgggcgacg atctcagaac agttaaacga
     5281 gtcgaacaag aagcaggcaa agtttagagc actgcccctc cgcactcaaa aaagaaaaaa
     5341 ctaggaggaa aataaaattc tcaaccacac aaacacataa acacatacaa atacaaatac
     5401 aagcttattt acttgacatc gcgcgatctt ccactattca gcgccgtccg ccctctctcg
     5461 tgttttttgt ttacgcgaca actatgcgaa atccggagca acgggcaacc gtttggggaa
     5521 agaccacacc cacgcgcgat cgccatggca acgaggtcgc acacgcccca cacccagacc
     5581 tccctgcgag cgggcatggg tacaatgtcc ccgttgccac agacaccact tcgtagcaca
     5641 gcgcagagcg tagcgtgttg ttgctgctga caaaagaaaa tttttcttag caaagcaaag
     5701 gaggggaagc acgggcagat agcaccgtac catacccttg gaaactcgaa atgaacgaag
     5761 caggaaatga gagaatgaga gttttgtagg tatatatagc ggtagtgttt gcgcgttacc
     5821 atcatcttct ggatctatct attgttcttt tcctcatcac tttccccttt ttcgctcttc
     5881 ttcttgtctt ttatttcttt ctttttttta attgttccct cgattggcta tctaccaaag
     5941 aatccaaact taatacacgt atttatttgt ccaattacca gatctatgac gacgaccaag
     6001 aggttgaagt ttgagaatct gagaatccaa ttctccaatg ccatagtagg aaacttttta
     6061 aggttaccgc attcaattat aaacgtgtta gagtcaacca actacgctat ccaggaattc
     6121 ggtatagcag tccattctca taattcagat atacctattg ttcatcttgg atgggatggc
     6181 catgattctg gatcgagtga gaatgttgtt cttatcaatc ctgttttggc cacagtatat
     6241 gatttaaatc aaaagtctcc attggtggac ttatacatcc agcgatatga ccacacacac
     6301 ctggctacgg aggtgtatgt cacgccagaa acgagtgatg attgggaaat tatcgatgcc
     6361 aatgcgatga ggttccaaaa tggtgagatt ttgcaccaaa cccgtatagt taccccaggt
     6421 gagactttga tttgttattt ggaaggaatt gttacaaagt tcaaaattga cagggtggag
     6481 ccatcaatga agtctgcgag gatcacagat ggttccttgg ttgttgtggc accaaaagtc
     6541 aataaaacac gtttggtgaa ggctgagtat ggtcatagta ataagacaat actaaaaaat
     6601 ggggctatcc aacttttaaa gaaggtaata ttgaggagta ctgtgtgcaa aatggacttc
     6661 cctaaagata acctttttgt cgtatatatc agtgacgggg cacaattgcc ttctcagaag
     6721 ggctacgctt ctatcgtaaa atgtagctta aggcagtcca agaaaagcga ttcagataat
     6781 aaatcagttg gtatcccttc gaagaagatt ggggttttta taaaatgtga tagtcagata
     6841 cccgaaaatc acattgcatt aagctctcat ctttgggatg cattctttac gcacccaatg
     6901 aacggagcaa aaatcaaact agagtttcta caaatgaatc aagcaaatat aatatccgga
     6961 agaaatgcaa cagtcaatat caaatatttt ggcaaggacg ttcctaccaa gagtggggat
     7021 cagtattcta agttgttggg cgggagctta ttgaccaata acttaatact tccaacagag
     7081 caaattataa tcgaaataaa aaaaggtgaa tccgagcagc agttgtgtaa tttgaatgaa
     7141 atttccaatg aatccgttca atggaaagta acccaaatgg gtaaagaaga agttaaagat
     7201 attatcgaaa ggcatttgcc taaacattac cacgtcaaag aaactggcga agtttcgcgc
     7261 actagtaaag atgaagatga tttcattacg gtaaatagta tcaagaaaga aatggtcaat
     7321 tatttgacat cgcctattat tgccacacca gctattatat tagatggtaa gcaagggatc
     7381 ggtaaaacaa ggttattaaa agagcttata aatgaagtgg aaaaagatca tcacatcttc
     7441 gttaaatatg cggattgtga aacattgcac gagacatcaa atttagataa aacccaaaaa
     7501 ttaattatgg aatggtgttc tttttgttat tggtatggtc cttctttgat tgtgttggat
     7561 aacgttgagg ctctatttgg aaaacctcaa gccaatgacg gagatccttc taataatggc
     7621 caatgggata atgcaagcaa acttttgaat tttttcatca atcaggtgac aaagatattt
     7681 aacaaggata ataaacgaat cagagtttta ttttcaggca agcagaaaac tcaaattaat
     7741 ccgctattgt ttgataagca ttttgtttca gaaacgtggt ctttgagagc acctgacaaa
     7801 catgcaagag cgaagttact ggagtatttt ttctcgaaaa accagatcat gaaactaaat
     7861 cgagatttgc aattcagtga tttgtcgttg gagacagaag gattttcgcc actagatttg
     7921 gaaatattca cagaaaagat tttctacgac ttgcagttgg aaagagattg tgataatgtt
     7981 gtaacaaggg aacttttttc gaagtcgctt agtgcgttta caccatctgc gttgcgtgga
     8041 gtaaagctaa caaaagaaac gaacatcaaa tggggggata ttggtgcttt agcaaatgcc
     8101 aaagatgtcc ttctagaaac cttagagtgg cccacaaaat atgagcctat tttcgttaat
     8161 tgtcccctaa gattgagatc aggaatcttg ctttacggtt atcctggttg tggtaaaacg
     8221 cttctggcga gcgccgtggc acaacaatgt gggttaaact ttatctccgt taagggacca
     8281 gagattttaa acaagtttat aggtgccagc gaacaaaaca taagagaatt gtttgaaagg
     8341 gcacagtccg tcaaaccctg tattctattt tttgacgagt tcgattctat tgcgccaaag
     8401 agagggcatg actccactgg tgtcacagac cgtgtagtca atcaattatt gacccaaatg
     8461 gatggtgccg agggccttga tggtgtatat atactagcag ctacaagtag acctgatttg
     8521 attgatagcg cattgttaag accgggaaga ttagacaaaa gtgtgatctg taatataccg
     8581 actgaatcag agaggttaga tatcttgcaa gctatcgtca attcaaaaga caaggatacg
     8641 ggacaaaaaa aatttgcatt ggagaaaaat gcagacttga agttgatcgc tgaaaagaca
     8701 gctggttttt cgggtgctga tttacagggg ctctgctaca atgcttattt gaaatctgta
     8761 cataggtggc tgtctgctgc agaccaatcg gaagtggtac ccggcaatga taatatagaa
     8821 tacttcagta tcaatgagca cgggcgtaga gaagaaaata gactacggct aaagactcta
     8881 ttgcagcaag atgtggtgca cgagacaaag acaagcactt cagctgcttc tgagctgaca
     8941 gctgtggtca caataaacga tttattggaa gcctgtcaag agacaaaacc tagtatttca
     9001 acaagtgagt tggtcaaatt aagggggatt tacgataggt tccagaagga taggaacggt
     9061 gagatgccca atggtgagaa ttccatcgac attggtagcc gactctccct tatgtgagga
     9121 tcctaactcg agagtgcttt taactaagaa ttattagtct tttctgctta ttttttcatc
     9181 atagtttaga acactttata ttaacgaata gtttatgaat ctatttaggt ttaaaaattg
     9241 atacagtttt ataagttact ttttcaaaga ctcgtgctgt ctattgcata atgcactgga
     9301 aggggaaaaa aaaggtgcac acgcgtggct ttttcttgaa tttgcagttt gaaaaatgct
     9361 gtttatatat ggagtcccgc cagcataacg ccttgtgaaa ttgcaaatat ggtgatttga
     9421 aacgtttcct agtgcagcag gatcacagat aacgtgtaaa gggcttagca gttgataatc
     9481 ctctctagtt aagacctaaa caaaatgctg tcactaaccg tagtattaaa tgacacactt
     9541 tggtgacttt cgttaatggg gatgtggtag tggccattgc caataaacaa aaagaacagg
     9601 gaaagaagta gaaagtgata taagtttgct tgccactttt cgtttttcac gaaaaaaaca
     9661 ggcgaaaaaa aatgctagac aagtacccgg ctgaatcaca cctcgttaac agtgactttc
     9721 ggtgacagat acccgattgg gcacccggct ggtaagttat gatagaaagc caacgctgta
     9781 ctattggctt agctatggca atattttgat tatcagctag ttttattaac gttataatta
     9841 gtgtaaccag tttttcatct atttcattta tttcatttat ttactttaat tgcagatccc
     9901 cctaacgcgt ttaaagcttt tattcactag cttatgtatt ttttatagga aacgcgacgc
     9961 gtaacatcgc gcaaatgaag gttttgatgt attataatga ggtattcttc cttatataca
    10021 tcgatgaaaa gcgttgacag catacattgg aaagaaatag gaaacggaca ccggaagaaa
    10081 aaatagatct atgaaggcat cgcttacgtt tagtctctcc ggaatatacg ccccctgtag
    10141 catttctagg gatatatatc tcgaatacgg cgataaaaaa gctgaatgtt tatatgggac
    10201 aattagattg cctcagtacg gtccaggttg cacgcctggt aaaattgtac attgtgtcct
    10261 ggatgattca ctgcccttct gtagcattgt tgtaccttcc aaactttttg ggtttatgcc
    10321 gacacagccc actatggatt tttgctattt tgaacccata ttagacaatg tagtacctgt
    10381 actggatagc gtaacgtttc tgattaatga acaattgtac tcaaaactaa tggacctacc
    10441 gcaagagatg caacaaatac aatttttgca ttacaaatat aacatcaact ccatggaaac
    10501 cgtggttcat tctcgagata ttttaacatc tggtctttgc caaattttaa actgctcacc
    10561 tttcccgcag ggccttgttg attttacaga aacccaattg atattagtaa atgatactga
    10621 gcagaaacta agtgctctaa aatatgctaa cgaagatgaa gaatatgcat tacctaaaat
    10681 agggacaaat tctgctttat caattgatct tgaatctctc ccctgcacaa tttcaagaga
    10741 tcttttgcga cctgcacctc acatcaatga tgataattca atatatgcct ttacagacgc
    10801 tgaaactcta ctgcgcctgg atgtcacaag tggatcgttt ataacagtgt caaatatggg
    10861 ttgcgtcagg ctagttaagc tgtttgtttt actactcccc aacggcttta aaaaaagaac
    10921 aatatacgca cctcccaaaa taatagcaag ctttccagac tgcagcgtcg tgacaatatc
    10981 aaagtccaat ataggccata cagatattcc cattgctaat caagttttca tctcaagagt
    11041 tggaggctgg cttcaatctc agaaatgctt tcaaaatatt attctaacaa ccttgaaaaa
    11101 gttttttagc gagagcaaaa ggattctttg tcaaaatgat ttaatcccaa tcgcttttga
    11161 ttcaagtatg gctgatctca atatagcaga ggaaaatgat gagtctgatg acgaagatga
    11221 gctagggcag tactacaaga acgattccct tgtatggttc tttgtcacaa gcgcagagct
    11281 cgattgcttc tctaaagata attctcattt tattattgat cccaatcgga cgaagctaat
    11341 aacaactaat atcactaaca gaagaccatt acccttgagt agatccaatc ttcagagata
    11401 ttacggtttt gctgaaactt tttactatga tttgcatatc ttcccctatg taagacaatt
    11461 ggttaacata ttagaaactt ccttcaattg ttctcaaagg ggaatcaccc taaatgcctc
    11521 tgtgttactt cattccacta caaacaatgt gggcaaggct acaatggtga gatttgcttc
    11581 aaaatatctt ggtatacatc tactggagat agattgcctt tcgctcactt caaattcaag
    11641 acaactggat tcaacatcta agattattgg ttacattagg gctaaatgtg aaaatgtcct
    11701 accgtacgca tcacctgctg tgattttttt ggctcatttg gattcaattt tgcttgacgt
    11761 aaacgctaat caagatccag aggcaatcaa attacaaaaa tctataaact tcgaaatgtc
    11821 taaacttctg gacgatttta cgtttaaatt cccaggaact acatttgttg gctccgtaaa
    11881 taacatagat aacgtgcctt ccagctttag atcacatatg agatttgaga ttctcgtacc
    11941 cgttccgtct gaagcacaga gactgcgtat ctttcagtgg tacttatctt cacatgaact
    12001 aaacagagat gttcaacaaa aggttccagt atcttacatg gataatattt cattttcatc
    12061 actctcttca tattctgctg gtttaactcc tttagacatc aaatcaattg tggaaacggc
    12121 acgaatgacg gctactgcgc gtttttacca agaatcaaag aagtgtggat ggcttccgca
    12181 atcaattttg atcactcagg aggatttatc aaaagctact tcgaaagcta ggaacgaatt
    12241 ctcggtttcg attggtgccc cacaaatccc taacgtaact tgggatgata taggtggtat
    12301 tgattttgtt aaaggtgaaa tattggacac aatagacatg ccgctaaaac atcctgaatt
    12361 atttacctca ggtatgaaaa agagaagcgg tattcttttt tatggtccac cgggtacagg
    12421 taaaactcta atggctaagg ccattgcaac aaatttttct ttaaattttt ttagtgttaa
    12481 aggccctgaa ctgttgaata tgtacattgg tgagagtgaa gctaatgtgc gcagagtgtt
    12541 tcaaaaggcg agagaggcta aaccttgtgt catatttttc gatgaaatcg attcagtagc
    12601 acccaaacgt ggtaatcaag gtgattcggg tggtgttatg gatcgtatcg tttcacagtt
    12661 actagcagag ttagatggta tgagtaccga cgctgacggt gtatttgtta tcggagcaac
    12721 aaacagacca gacttattgg acgaagcact actaagacca ggacgattcg ataaattgtt
    12781 atatttaggc attccggata cggataccaa acaattgaat attttagagg ctctgactcg
    12841 caaattcgtg ctcgacaacg acgtaaagct tattgagttg gcaaagctat gtccatttaa
    12901 ttacaccggg gcagattttt atgctctctg ctcagatgca atgcttaacg ccatgtcaag
    12961 aattgcacgc atggtagaaa aaaaagtttc tcagcataac gaattgacgg gagagaatat
    13021 ttctacacgt cgctggtttg ataagattgc gacaaaagag gatactaagg ttgtcgtaaa
    13081 aatggaagac ttcttgaaag cacaagagca gctcacccca agtgtgtcgc gggctgaact
    13141 gaatcattat gaagcggtga gagctaattt tgaaggtgct taaggatcct aactcgaggt
    13201 gaatttactt taaatcttgc atttaaataa attttctttt tatagcttta tgacttagtt
    13261 tcaatttata tactatttta atgacatttt cgattcattg attgaaagct ttgtgttttt
    13321 tcttgatgcg ctattgcatt gttcttgtct ttttcgccac atgtaatatc tgtagtagat
    13381 acctgataca ttgtggatgc tgagtgaaat tttagttaat aatgctgagc agttacagag
    13441 atgttacgaa ccactagtgc actgcagtac acggtttcct tgaaattttt ttgattcggt
    13501 aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg
    13561 catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac
    13621 aaaaacgtgc aggaaacgaa gataaatcat gtcgaaagct acatataagg aacgtgctgc
    13681 tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa
    13741 cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt
    13801 aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga
    13861 gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga
    13921 cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag
    13981 aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag
    14041 cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt tgatgttagc
    14101 agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta ctgttgacat
    14161 tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag
    14221 agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg acaagggaga
    14281 tgcattgggt caacagtata gaaccgtgga tgatgtggtt tctacaggat ctgacattat
    14341 tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta
    14401 cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact aaaaaactgt
    14461 attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca
    14521 gttattaccc gagtagagca cttgaatcca ctgccccggg aatctcggtc gtaatgattt
    14581 ctataatgac gaaaaaaaaa aaattggaaa gaaaaagctt catggccttt ataaaaagga
    14641 actatccaat acctcgccag aaccaagtaa cagtatttta cggggcacaa atcaagaaca
    14701 ataagacagg actgtaaaga tggacgcatt gaactccaaa gaacaacaag agttccaaaa
    14761 agtagtggaa caaaagcaaa tgaaggattt catgcgtttg tactctaatc tggtagaaag
    14821 atgtttcaca gactgtgtca atgacttcac aacatcaaag ctaaccaata aggaacaaac
    14881 atgcatcatg aagtgctcag aaaagttctt gaagcatagc gaacgtgtag ggcagcgttt
    14941 ccaagaacaa aacgctgcct tgggacaagg cttgggccga taaggtgtac tggcgtatat
    15001 atatctaatt atgtatctct ggtgtagccc atttttagca tgtaaatata aagaccgagc
    15061 ggccgcgtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
    15121 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
    15181 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
    15241 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
    15301 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt
    15361 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
    15421 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgaggcgaa atacgcgatc
    15481 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
    15541 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
    15601 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
    15661 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
    15721 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
    15781 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
    15841 taaatcagca tccatgttgg aatttaatcg cggcctggag caagacgttt cccgttgaat
    15901 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
    15961 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg
    16021 ttgaataaat cgaacttttg ctgagttgaa ggatcagtca tgaccaaaat cccttaacgt
    16081 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    16141 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    16201 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    16261 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    16321 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    16381 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    16441 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    16501 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    16561 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    16621 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    16681 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    16741 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    16801 cctgattctg tggataaccg tgcggccgcc aatgctaaat tcgagtgaaa cacaggaaga
    16861 tcagaaaatc ctcatttcat ccatattaac aataatttca aatgtttatt tgcattattt
    16921 gaaactaggc aagacaagca acgaaacgtt tttgaaaatt ttgagtattt tcaataaatt
    16981 tgtagaggac tcagatattg aaaaaaagct acagcaatta atacttgata agaagagtat
    17041 tgagaagggc aacggttcat catctcatgg atctgcacat gaacaaacac cagagtcaaa
    17101 cgacgttgaa attgaggcta ctgcgccaat tgatgacaat acagacgatg ataacaaacc
    17161 gaagttatct gatgtagaaa aggattaaag atgctaagag atagtgatga tatttcataa
    17221 ataatgtaat tctatatatg ttaattacct tttttgcgag gcatatttat ggtgaaggat
    17281 aagttttgac catcaaagaa ggttaatgtg gctgtggttt cagggtccat aaagcccaca
    17341 tggataacat tacccctgaa ttcgcatcta gactga
//
