LOCUS       sequence-432322-       10869 bp    DNA     circular SYN 15-APR-2025
DEFINITION  pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express 
            HaloTag-MDC1 with a deletion of the PST repeat region in human 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Heyza JR, Mikhova M, Bahl A, Broadbent DG, Schmidt JC
  TITLE     Systematic analysis of the molecular and biophysical properties of 
            key DNA damage response factors.
  JOURNAL   Elife. 2023 Jun 21;12:e87086. doi: 10.7554/eLife.87086.
  PUBMED    37341699
REFERENCE   2  (bases 1 to 10869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-06-21"; volume: "12:e87086. doi"; pages: " 
            10.7554/eLife.87086"
COMMENT     Sequence Label: Halo-MDC1 PST deletion
FEATURES             Location/Qualifiers
     source          1..10869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1061..1070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1070..1135
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    1136..1169
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1178..2068
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             2081..2101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(6820..6838)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    6870..6991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6881..6900)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6935..6954
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7236..7593
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7236..7427
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7259..7279)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7444..7579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7644..8438
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7698..7717)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8308..8327
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8502..8550
                     /note="synthetic polyadenylation signal"
     promoter        8706..8810
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8811..8879,8880..9671)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9029..9048)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9829..10417)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9909..9928)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10533..10816
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg actacaaaga
     1081 ccatgacggt gattataaag atcatgatat cgattacaag gatgacgatg acaagataac
     1141 ttcgtataat gtatgctata cgaagttatc cggtaccatg gcagaaatcg gtactggctt
     1201 tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg tcgatgttgg
     1261 tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct cctacgtgtg
     1321 gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag acctgatcgg
     1381 tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg tccgcttcat
     1441 ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc acgactgggg
     1501 ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag gtattgcatt
     1561 tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg cccgcgagac
     1621 cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc agaacgtttt
     1681 tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg agatggacca
     1741 ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct tcccaaacga
     1801 gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat acatggactg
     1861 gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg ttctgatccc
     1921 accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg tggacatcgg
     1981 cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg agatcgcgcg
     2041 ctggctgtcg acgctcgaga tttccggcga gccaaccact gaggatctgt actttcagag
     2101 cgataacgcg atcgcttcca tggaggacac ccaggctatt gactgggatg ttgaagaaga
     2161 ggaggagaca gagcaatcca gtgaatcctt gaggtgtaac gtggagccag tagggcggct
     2221 acatatcttt agtggtgccc atggaccaga aaaagatttc ccactacacc tcgggaagaa
     2281 tgtggtaggc cgaatgcctg actgctctgt ggccctgccc tttccatcta tctccaaaca
     2341 acatgcagag attgaaatct tagcctggga caaggcacct atcctccgag actgtgggag
     2401 ccttaatggt actcaaatcc tgagacctcc taaggttttg agccctgggg tgagtcaccg
     2461 tctgagggac caggaattga ttctctttgc tgacttgctc tgccagtacc atcgcctgga
     2521 tgtctctctg ccctttgtct cccggggccc tctgacagta gaagagacac ccagagtaca
     2581 gggagaaact caaccccaga ggcttctgtt ggctgaggac tcggaggagg aagtagattt
     2641 tctttctgaa aggcgtatgg taaaaaaatc aaggaccaca tcttcctctg tgatagttcc
     2701 agagagtgat gaagaggggc attccccggt cctgggcggc cttgggccgc cttttgcctt
     2761 caatttgaac agtgacacag atgtggaaga aggtcagcaa ccagccacag aggaggcctc
     2821 ctcagctgcc agaagaggtg ccactgtaga ggcaaagcag tctgaagctg aagttgtaac
     2881 tgaaatccag cttgaaaagg atcagccttt agtgaaggag agggacaatg atacaaaagt
     2941 caagaggggt gcagggaatg gggtggttcc agctggggtg attctggaga ggagccaacc
     3001 tcctggagag gacagtgaca cagatgtgga tgatgacagc aggcctcctg gaaggccagc
     3061 tgaggtccat ttggaaaggg ctcagccttt tggcttcatc gacagcgaca ctgatgcgga
     3121 agaagagagg atcccagcaa ccccagttgt cattcctatg aagaagagga agatcttcca
     3181 tggagtaggt acaaggggtc ctggagcacc aggcctggcc catctgcagg agagccaggc
     3241 tggtagtgat acagatgtgg aagaaggcaa ggccccacag gctgtccctc tggagaaaag
     3301 ccaagcttcc atggttatca acagcgatac agatgacgag gaagaagtct cagcagcgct
     3361 gactttggca catctgaaag agagccagcc tgctatatgg aacagagatg cagaagagga
     3421 catgccccaa cgtgtggtcc ttctgcagcg aagccaaacc accactgaga gagacagtga
     3481 cacagacgtg gaggaggaag agctcccagt ggaaaataga gaagctgtcc tcaaggatca
     3541 cacaaagatt agagcccttg ttagagcaca ttcagaaaag gaccaacctc cttttgggga
     3601 cagtgatgac agtgtggaag cagataagag ctcacctggg atccacctgg agagaagcca
     3661 agcctccacc acagtggaca tcaacacaca agtggagaag gaagtcccgc cagggtcagc
     3721 cattatgcat ataaagaagc atcaggtgtc tgtggagggg acaaatcaaa cagatgtgaa
     3781 agcagttggg ggaccagcaa agctgcttgt ggtatctcta gaggaagcct ggcctctgca
     3841 tggggactgt gaaacagatg cagaggaggg cacctcccta acagcctcag tagttgcaga
     3901 tgtaagaaag agccagcttc cagcagaagg ggatgctggg gcagagtggg ctgcagctgt
     3961 tcttaagcag gagagagctc atgaggtggg ggcccagggt gggccacctg tggcacaagt
     4021 ggagcaggac ctccctatct caagagagaa cctcacagat ctggtggtgg acacagacac
     4081 tctaggggaa tccacccagc cacagagaga gggagcccag gtccccacag gaagggagag
     4141 agaacaacat gtgggtggga ccaaggactc tgaagacaac tatggtgatt ctgaagatct
     4201 ggacctacaa gctacccagt gctttctgga gaatcagggc ctggaagcag tccagagcat
     4261 ggaggatgaa cctacccagg ccttcatgtt gactccaccc caagagcttg gcccttccca
     4321 ttgcagcttc cagacaacag gtaccctaga tgaaccatgg gaggtcctgg ctacacagcc
     4381 attctgtctg agagagtctg aggactctga gacccagcct tttgacacgc accttgaggc
     4441 ctatggacct tgcctgtctc cacctagggc aataccagga gaccaacatc cagagagccc
     4501 agttcacaca gagccaatgg ggattcaagg cagagggagg cagactgtgg ataaagtcat
     4561 gggtatacca aaagaaacag cagagagggt gggccctgag agagggccat tggagagaga
     4621 aactgagaaa ctgctaccag aaagacagac agatgtgaca ggagaggaag aattaaccaa
     4681 ggggaaacag gacagagaac aaaaacagtt gttagctaga gacacccaga gacaagaatc
     4741 tgacaaaaat ggggaaagtg caagtcctga aagagatagg gagagtttga aggtagaaat
     4801 tgagacatct gaggaaatac aagagaaaca agtacagaag cagacccttc caagcaaagc
     4861 atttgagaga gaagtagaga gaccagtagc aaacagagag tgcgatccag ccgagttaga
     4921 agagaaggtg cccaaagtga tcctggagag agatacacag agaggggagc cagagggagg
     4981 gagccaggac cagaaagggc aggcctccag cccaacacca gagcctgggg tgggggcggg
     5041 ggaccttccg ggacctacct cagcccccgt accttctggg agccagtcag gtggaagggg
     5101 atccccagtg agccccagga ggcatcagaa aggcctcctg aattgcaaga tgccacctgc
     5161 tgagaaggct tccaggatca gagctgctga gaaggtttcc aggggcgatc aggaatctcc
     5221 agatgcttgt ctgcctcctg cagtacctga agccccagcc ccaccccaaa agccccttaa
     5281 ctctcagagc cagaaacatc ttgcacctcc gccccttctt tctccccttt taccttctat
     5341 caagccaacc gttcgtaaga ccaggcaaga tgggagtcag gaagctccag aggctccctt
     5401 gtcctcagag ctggagcctt tccacccaaa gcctaaaatt agaactcgga agtcctccag
     5461 aatgacaccc tttccagcta cctctgctgc ccctgagccc caccctttta cccccacaga
     5521 ccagtccgtc acccctgagg ccatagctca gggtggtcag agcaaaacac tgaggtcttc
     5581 cacagtaaga gctatgccgg ttcctaccac ccctgaattc caatctcctg tcaccacaga
     5641 ccagcctatt tcccctgagc ctattactca acccagttgc atcaagaggc agagagccgc
     5701 tgggaaccct ggctccctcg cagctcccat tgaccataag ccttgctctg cacccttgga
     5761 acctaaatcc caggcctcaa ggaaccaaag atggggagca gtgagagcag ctgaatccct
     5821 tacagccatt cctgagcctg cctctcccca gcttcttgag acaccaattc atgcctccca
     5881 gatccaaaag gtggaaccag caggtagatc taggttcacc ccggagctcc agcctaaggc
     5941 ctctcaaagc cgcaagaggt ctttagctac catggattca ccaccacatc aaaaacagcc
     6001 ccaaagaggg gaagtctccc agaagacagt gattatcaag gaagaggaag aagatactgc
     6061 agagaagcca gggaaggaag aggatgtcgt gactccaaaa ccaggcaaga gaaagagaga
     6121 ccaggcagag gaggagccca acagaatacc aagccgcagc ctccgacgga ccaaacttaa
     6181 ccaagaatca acagccccca aagtgctctt cacaggagtg gtggatgctc ggggagagcg
     6241 ggctgtgctg gcactggggg gaagtctggc tggttcagcg gcagaggctt cccacctggt
     6301 cactgatcgc atccgccgga cagtcaagtt cctgtgtgcc ctggggcggg gaatccccat
     6361 tctgtccctg gactggctgc atcagtcccg caaggctggt ttcttcttac ccccggatga
     6421 atatgtggtg accgaccctg agcaagagaa gaactttggc tttagccttc aagacgcact
     6481 gagcagggct cgggagcgaa ggctgctaga gggctatgag atctatgtga cccctggagt
     6541 ccagccacca ccacctcaga tgggagagat tattagctgc tgtggaggca catacctacc
     6601 cagcatgcct cggtcctata agcctcagag agttgtgatc acatgccctc aggacttccc
     6661 tcattgctcc attccactac gggttgggct gcccctcctc tcgcctgagt tcctgctgac
     6721 tggagtgctg aagcaggaag ccaagccaga ggcctttgtc ctctcccctt tggagatgtc
     6781 atccacctga gcggccgctt gctgagttgg ctgctgccac cgctgagcaa taactagcat
     6841 aaccccttgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac
     6901 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     6961 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     7021 tcaggttcag ggggagatgt gggaggtttt tttaagcaag taaaacctct acaaatgtgg
     7081 taaaatcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct
     7141 tacgcatctg tgcggtattt cacaccgcat acgcggatct gcgcagcacc atggcctgaa
     7201 ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg
     7261 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7321 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7381 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7441 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7501 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7561 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg attcttctga cacaacagtc
     7621 tcgaacttaa ggctagagcc accatgattg aacaagatgg attgcacgca ggttctccgg
     7681 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     7741 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     7801 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     7861 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     7921 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     7981 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     8041 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     8101 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     8161 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     8221 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     8281 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     8341 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     8401 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     8461 gaccgaccaa gcgacgccca acctgccatc acgatggccg caataaaata tctttatttt
     8521 cattacatct gtgtgttggt tttttgtgtg aatcgatagc gataaggatc ctctttgcgc
     8581 ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt
     8641 tctgcggact ggctttctac gtaatggttt cttagacgtc aggtggcact tttcggggaa
     8701 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8761 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8821 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8881 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8941 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     9001 ttccaatgat gagcactttc aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     9061 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     9121 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9181 ccataaccat gagtgataac actgcggcca acttacttct gacaactatc ggaggaccga
     9241 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9301 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9361 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9421 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9481 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9541 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9601 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9661 agcattggta attcgaaatg accgaccaag cgacgcccaa ccggtatcag ctcactcaaa
     9721 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9781 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9841 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9901 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9961 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10021 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10081 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10141 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10201 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10261 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10321 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10381 caagcagcag attacgcgca gaaaaaaagg atttcaagaa gatcctttga tcttttctac
    10441 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10501 aaaaaggatc ttcacctaga tccttttata gtccggaaat acaggaacgc acgctggatg
    10561 gcccttcgct gggatggtga aaccatgaaa aatggcagct tcagtggatt aagtgggggt
    10621 aatgtggcct gtaccctctg gttgcatagg tattcatacg gttaaaattt atcaggcgcg
    10681 attgcggcag tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg
    10741 ctgaacgcgt tttagcggtg cgtacaatta agggattatg gtaaatccac ttactgtctg
    10801 ccctcgtagc catcgagata aaccgcagta ctccggccac gatgcgtccg gcgtagagga
    10861 tcgagatct
//
