LOCUS       sequence-432799-       10462 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes SARS-CoV-2 spike Q493R for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA)
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3839..4427
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3892..3912)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4310..4321)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4480..4601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4491..4510)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4545..4564
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4716..4762
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4792..4928
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4973..5044
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5089..5285
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5136..5271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5299..6093
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5353..5372)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5963..5982
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6104..6328
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6524..7112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6604..6623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7858..7926,7927..8718)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8076..8095)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8731..8867
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9019..9090
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9095..9141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9159..9207
                     /note="synthetic polyadenylation signal"
     enhancer        9288..9591
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9593..9795
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9746..9766
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9929..10404
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atgtttgtgt tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcacaact
       61 agaacgcagc tcccacccgc ttacacgaac tcttttactc gtggagtata ctatcccgat
      121 aaagtcttta ggtccagtgt ccttcattct actcaagacc ttttcctgcc attcttctca
      181 aatgtcacct ggttccatgc tatccacgtt tctggcacca acggaactaa acgatttgat
      241 aatcccgtct tgccatttaa cgatggcgta tactttgcct caacggagaa gtccaacatt
      301 atccggggat ggatatttgg caccaccctg gactccaaga cacagtctct cctcattgtg
      361 aacaacgcca ccaatgtcgt catcaaagtc tgtgaatttc aattttgtaa cgatcccttt
      421 ctcggggttt attatcataa gaataacaag tcttggatgg agtcagagtt ccgcgtttac
      481 agttccgcta acaactgtac ttttgagtac gtttcccaac catttctcat ggatcttgaa
      541 gggaagcaag gcaacttcaa gaatctccgc gaatttgtgt ttaagaatat agacggctac
      601 ttcaaaattt atagcaagca taccccgata aatctggttc gggatctccc tcaaggcttc
      661 tccgcgttgg aacccctcgt ggacttgccg attggcatca acatcacccg gttccaaacc
      721 cttcttgccc ttcatcgaag ctacctcacg cctggcgaca gtagtagtgg ttggacagcg
      781 ggcgcagcag cctactatgt gggatatttg caaccacgca cctttcttct caaatataac
      841 gaaaacggaa caattaccga tgccgtagac tgtgccctcg atcccctgtc cgaaactaaa
      901 tgcacactca agtccttcac agttgagaaa ggcatatacc aaaccagtaa cttccgcgtc
      961 caacccaccg aatccattgt tcgattccct aacatcacca atttgtgtcc tttcggtgaa
     1021 gtatttaatg ccactcgatt tgcatcagtt tatgcctgga atcgcaagcg aatatcaaat
     1081 tgtgttgcag attatagtgt cctgtataat agcgcatcat tcagcacttt taagtgttat
     1141 ggggtcagcc ctacgaaatt gaatgacttg tgttttacca acgtttacgc agattctttc
     1201 gtcatacgcg gcgacgaagt ccgacagatt gcaccggggc aaaccggaaa gatagcagac
     1261 tataattata agttgccgga tgacttcacc ggctgcgtta tagcatggaa ctccaataat
     1321 ctcgattcaa aagtcggcgg caactataat tatctttacc gcctgtttcg caagtcaaat
     1381 ctgaagccct ttgaacgtga catctccacc gaaatttatc aagctggcag caccccatgt
     1441 aatggagtgg aagggtttaa ctgttatttt ccccttcgat catacggatt tcaaccaaca
     1501 aatggggtgg gctatcaacc atatcgggtg gtagtcttga gctttgagct gctccacgca
     1561 ccggctacag tttgcggacc gaagaaaagc actaacctgg tgaagaataa atgtgtgaac
     1621 ttcaacttta atgggctcac cggaacaggg gtgctgacag aatcaaacaa gaaatttctc
     1681 ccttttcaac aatttggtag agacattgcc gataccacag atgccgtccg cgatccgcaa
     1741 acactggaaa tcctcgatat aacaccctgt agcttcggcg gggtcagcgt gataactccc
     1801 ggaacgaaca catccaacca agtggctgtc ctgtatcaag atgtcaactg taccgaagtc
     1861 cctgtggcga tacacgccga ccaactgaca cccacctgga gagtctactc aacaggttcc
     1921 aatgtctttc aaactagagc gggttgtctc attggcgctg agcatgtcaa taattcatac
     1981 gaatgtgata ttcctattgg ggcaggaatc tgtgcgtctt atcaaactca aacgaactca
     2041 ccacggcgcg ctcgctcagt agccagccag tccataatcg cctataccat gagtctcggg
     2101 gctgagaact ccgtggctta ttcaaacaat tccatcgcaa tcccaactaa ctttaccatt
     2161 agcgttacca cggaaatctt gcccgtcagc atgactaaga cgagcgtaga ctgtacgatg
     2221 tatatctgtg gggattccac cgagtgtagc aacctcttgc ttcaatacgg gtctttctgt
     2281 actcaactca accgagcatt gaccggaatt gcggtcgaac aagacaagaa tacccaagag
     2341 gtgttcgcac aagtaaagca aatatacaaa acgccgccaa tcaaagattt tggcgggttt
     2401 aattttagcc aaatactgcc agacccatct aagccttcaa aacgcagctt tatcgaggat
     2461 ctgctgttca acaaagttac gttggctgac gcgggtttca ttaagcagta tggggattgt
     2521 ttgggagaca ttgcggcccg cgaccttatc tgtgctcaaa aattcaatgg gttgacagtt
     2581 ctgccccctc tgctcactga tgagatgatt gcacagtata catcagcatt gttggcagga
     2641 accattacat ccggctggac ttttggcgcg ggtgcggccc ttcaaatacc tttcgctatg
     2701 caaatggctt acagattcaa tgggattggt gtcacgcaaa atgtattgta tgaaaatcag
     2761 aagctgatcg ctaatcaatt taacagcgcg attggcaaga tccaagattc actgtcaagc
     2821 acagcgtccg ccctcggcaa actccaagat gttgtgaatc aaaacgccca ggccctgaat
     2881 accctcgtga agcaactgag ttctaacttc ggcgcaatct ccagcgtcct caacgacata
     2941 ttgtccagac ttgataaggt ggaagccgag gtgcaaattg atcggctcat cacaggcagg
     3001 ctccagtcac tccaaactta tgtgacacaa cagttgatcc gggctgctga gatacgagca
     3061 agcgccaacc tggccgccac taaaatgtca gagtgcgtgc tcgggcagtc caaacgggtt
     3121 gacttttgtg gcaagggcta tcacttgatg tccttccccc aatccgcccc acatggagtg
     3181 gtctttctcc acgtcactta tgtccctgct caagagaaga actttacgac agctccagca
     3241 atctgtcacg atggcaaagc gcactttccc agggaaggcg ttttcgtcag taatggcacg
     3301 cattggtttg tgacccaaag gaatttctat gaaccccaaa tcataaccac tgacaataca
     3361 tttgtctcag gcaactgtga tgtggtcatt gggatcgtga acaacacggt atatgatccc
     3421 ctgcaacccg agttggatag cttcaaagag gaattggata aatactttaa gaatcatact
     3481 tcacccgatg ttgatctcgg ggacatatca ggaataaatg caagtgtcgt caacattcag
     3541 aaagagattg atcgccttaa tgaggtcgcc aagaacttga atgaatcttt gatcgacttg
     3601 caagaactgg gcaaatacga acaatatatt aaatggccct ggtacatttg gttgggcttt
     3661 atcgccggac ttatagctat cgttatggtc acaattatgt tgtgctgtat gactagctgt
     3721 tgttcctgct tgaaaggatg ttgcagttgc ggatcttgtt gtaaataaag gatcctctag
     3781 aagttgtctc ctcctgcact gactgactga tacaatcgat ttctggatcc gcaggcctaa
     3841 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3901 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3961 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4021 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4081 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4141 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4201 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     4261 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4321 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4381 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg tctagcttga
     4441 ctgactgaga tacagcgtac cttcagctca cagacatgat aagatacatt gatgagtttg
     4501 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     4561 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     4621 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     4681 acaaatgtgg tattggccca tctctatcgg tatcgtagca taaccccttg gggcctctaa
     4741 acgggtcttg aggggttttt tgtgcccctc gggccggatt gctatctacc ggcattggcg
     4801 cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg ccagattcag
     4861 caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta ccagaaattt
     4921 atccttaagg tcgtcagcta tcctgcaggc gatctctcga tttcgatcaa gacattcctt
     4981 taatggtctt ttctggacac cactaggggt cagaagtagt tcatcaaact ttcttccctc
     5041 cctaatctca ttggttacct tgggctatcg aaacttaatt aagcgatctg catctcaatt
     5101 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5161 ccgcccattc tccgccccat cgctgactaa ttttttttat ttatgcagag gccgaggccg
     5221 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5281 gcaaaggagg tagccaacat gattgaacaa gatggattgc acgcaggttc tcccgccgct
     5341 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     5401 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     5461 ggtgccctga atgaactcca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     5521 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5581 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5641 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5701 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5761 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5821 aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc acggcgatgc ctgcttgccg
     5881 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5941 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     6001 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     6061 gccttctatc gccttcttga cgagttcttc tagtatgtaa gccctgtgcc ttctagttgc
     6121 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6181 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6241 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6301 catgctgggg atgcggtggg ctctatggtt aattaaccag tcaagtcagc tacttggcga
     6361 gatcgacttg tctgggtttc gactacgctc agaattgcgt cagtcaagtt cgatctggtc
     6421 cttgctattg cacccgttct ccgattacga gtttcattta aatcatgtga gcaaaaggcc
     6481 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6541 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6601 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6661 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6721 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6781 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6841 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6901 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6961 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7021 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7081 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7141 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7201 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7261 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7321 tctgtctatt tcgttcatcc atagttgcat ttaaatttcc gaactctcca aggccctcgt
     7381 cggaaaatct tcaaaccttt cgtccgatcc atcttgcagg ctacctctcg aacgaactat
     7441 cgcaagtctc ttggccggcc ttgcgccttg gctattgctt ggcagcgcct atcgccaggt
     7501 attactccaa tcccgaatat ccgagatcgg gatcacccga gagaagttca acctacatcc
     7561 tcaatcccga tctatccgag atccgaggaa tatcgaaatc ggggcgcgcc tggtgtaccg
     7621 agaacgatcc tctcagtgcg agtctcgacg atccatatcg ttgcttggca gtcagccagt
     7681 cggaatccag cttgggaccc aggaagtcca atcgtcagat attgtactca agcctggtca
     7741 cggcagcgta ccgatctgtt taaacctaga tattgatagt ctgatcggtc aacgtataat
     7801 cgagtcctag cttttgcaaa catctatcaa gagacaggat cagcaggagg ctttcgcatg
     7861 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7921 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcgcga
     7981 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8041 gaacgctttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8101 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8161 gagtattcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8221 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgattgga
     8281 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8341 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8401 gtagcaatgg caacaacctt gcgtaaacta ttaactggcg aactacttac tctagcttcc
     8461 cggcaacagt tgatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8521 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8581 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8641 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8701 ctgattaagc attggtaacc gattctaggt gcattggcgc agaaaaaaat gcctgatgcg
     8761 acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg gcttccccaa
     8821 cttgcccact tccatacgtg tcctccttac cagaaattta tccttaagat cccgaatcgt
     8881 ttaaactcga ctctggctct atcgaatctc cgtcgtttcg agcttacgcg aacagccgtg
     8941 gcgctcattt gctcgtcggg catcgaatct cgtcagctat cgtcagctta cctttttggc
     9001 agcgatcgcg gctcccgaca tcttggacca ttagctccac aggtatcttc ttccctctag
     9061 tggtcataac agcagcttca gctacctctc aattcaaaaa acccctcaag acccgtttag
     9121 aggccccaag gggttatgct atcaatcgtt gcgttacaca cacaaaaaac caacacacat
     9181 ccatcttcga tggatagcga ttttattatc taactgctga tcgagtgtag ccagatctag
     9241 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     9301 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     9361 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     9421 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     9481 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     9541 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat gctgatgcgg
     9601 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     9661 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     9721 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     9781 tatataagca gagctggttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
     9841 tttgacctcc atagaagaca ccgggaccga tccagcctcc cctcgaagct tacatgtggt
     9901 accgagctcg gatcctgaga acttcagggt gagtctatgg gacccttgat gttttctttc
     9961 cccttctttt ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacacat
    10021 attgaccaaa tcagggtaat tttgcatttg taattttaaa aaatgctttc ttcttttaat
    10081 atactttttt gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata
    10141 atgatacaat gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg
    10201 gttaaggcaa tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg
    10261 taagaggttt catattgcta atagcagcta caatccagct accattctgc ttttatttta
    10321 tggttgggat aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc
    10381 atacctctta tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat
    10441 cactttggca aaggcggccg cc
//
