LOCUS       sequence-43281-e       13606 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13606)
  AUTHORS   Visanji NP, Wislet-Gendebien S, Oschipok LW, Zhang G, Aubert I, 
            Fraser PE, Tandon A
  TITLE     Effect of Ser-129 phosphorylation on interaction of alpha-synuclein 
            with synaptic and cellular membranes.
  JOURNAL   J Biol Chem. 2011 Oct 14;286(41):35863-73. Epub 2011 Aug 17.
  PUBMED    21849493
REFERENCE   2  (bases 1 to 13606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-10-14"; volume: "286(41)"; pages: "35863-73. 
            Epub 2011 Aug 17"
COMMENT     Sequence Label: pWPI/hPLK2WT/Neo
FEATURES             Location/Qualifiers
     source          1..13606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             3688..3705
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5599..5640
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5650..5667
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    5709..6276
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5727..6278
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5871..5888)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6098..6117
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6281..7075
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6335..6354)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6945..6964
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7251..7372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    7411..7999
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7464..7484)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7882..7893)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8168..8201)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8221..8401
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8426..8444)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8427..8444)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8734..8753)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8967..8986)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9086..9108
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9146..9164)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9232..9336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9337..9405,9406..10197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9555..9574)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10368..10956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10857..10876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11110..11127
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11197..11217)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11202..11531
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11382..11517
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12665..12730
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12860..12880
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13305..13439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttccgatcac gagactagcc
     3481 tcgaggtttc atatgtacag aggatccgcg accgcgacca tggagctttt gcggactatc
     3541 acctaccagc cagccgccag caccaaaatg tgcgagcagg cgctgggcaa gggttgcgga
     3601 gcggactcga agaagaagcg gccgccgcag ccccccgagg aatcgcagcc acctcagtcc
     3661 caggcgcaag tgcccccggc ggcccctcac caccatcacc accattcgca ctcggggccg
     3721 gagatctcgc ggattatcgt cgaccccacg actgggaagc gctactgccg gggcaaagtg
     3781 ctgggaaagg gtggctttgc aaaatgttac gagatgacag atttgacaaa taacaaagtc
     3841 tacgccgcaa aaattattcc tcacagcaga gtagctaaac ctcatcaaag ggaaaagatt
     3901 gacaaagaaa tagagcttca cagaattctt catcataagc atgtagtgca gttttaccac
     3961 tacttcgagg acaaagaaaa catttacatt ctcttggaat actgcagtag aaggtcaatg
     4021 gctcatattt tgaaagcaag aaaggtgttg acagagccag aagttcgata ctacctcagg
     4081 cagattgtgt ctggactgaa ataccttcat gaacaagaaa tcttgcacag agatctcaaa
     4141 ctagggaact tttttattaa tgaagccatg gaactaaaag ttggggactt cggtctggca
     4201 gccaggctag aacccttgga acacagaagg agaacgatat gtggtacccc aaattatctc
     4261 tctcctgaag tcctcaacaa acaaggacat ggctgtgaat cagacatttg ggccctgggc
     4321 tgtgtaatgt atacaatgtt actagggagg cccccatttg aaactacaaa tctcaaagaa
     4381 acttataggt gcataaggga agcaaggtat acaatgccgt cctcattgct ggctcctgcc
     4441 aagcacttaa ttgctagtat gttgtccaaa aacccagagg atcgtcccag tttggatgac
     4501 atcattcgac atgacttttt tttgcagggc ttcactccgg acagactgtc ttctagctgt
     4561 tgtcatacag ttccagattt ccacttatca agcccagcta agaatttctt taagaaagca
     4621 gctgctgctc tttttggtgg caaaaaagac aaagcaagat atattgacac acataataga
     4681 gtgtctaaag aagatgaaga catctacaag cttaggcatg atttgaaaaa gacttcaata
     4741 actcagcaac ccagcaaaca caggacagat gaggagctcc agccacctac caccacagtt
     4801 gccaggtctg gaacacccgc agtagaaaac aagcagcaga ttggggatgc tattcggatg
     4861 atagtcagag ggactcttgg cagctgtagc agcagcagtg aatgccttga agacagtacc
     4921 atgggaagtg ttgcagacac agtggcaagg gttcttcggg gatgtctgga aaacatgccg
     4981 gaagctgatt gcattcccaa agagcagctg agcacatcat ttcagtgggt caccaaatgg
     5041 gttgattact ctaacaaata tggctttggg taccagctct cagaccacac cgtcggtgtc
     5101 cttttcaaca atggtgctca catgagcctc cttccagaca aaaaaacagt tcactattac
     5161 gcagagcttg gccaatgctc agttttccca gcaacagatg ctcctgagca atttattagt
     5221 caagtgacgg tgctgaaata cttttctcat tacatggagg agaacctcat ggatggtgga
     5281 gatctgccta gtgttactga tattcgaaga cctcggctct acctccttca gtggctaaaa
     5341 tctgataagg ccctaatgat gctctttaat gatggcacct ttcaggtgaa tttctaccat
     5401 gatcatacaa aaatcatcat ctgtagccaa aatgaagaat accttctcac ctacatcaat
     5461 gaggatagga tatctacaac tttcaggctg acaactctgc tgatgtctgg ctgttcatca
     5521 gaattaaaaa atcgaatgga atatgccctg aacatgctct tacaaagatg taacctcgag
     5581 tctagagggc ccttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
     5641 cgtaccggtc atcatcacca tcaccattga ggatccacta gtcccgggaa actacgggct
     5701 gcaggaattc cgcccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     5761 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     5821 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     5881 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     5941 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     6001 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     6061 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     6121 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     6181 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac
     6241 ggggacgtgg ttttcctttg aaaaacacga tgataatacc atgattgaac aagatggatt
     6301 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     6361 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     6421 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     6481 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     6541 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     6601 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     6661 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     6721 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     6781 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     6841 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     6901 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     6961 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     7021 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     7081 actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     7141 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7201 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7261 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7321 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7381 gtataccgtc gacctcgagg gaattccgat aatcaacctc tggattacaa aatttgtgaa
     7441 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7501 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7561 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7621 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7681 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7741 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7801 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7861 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7921 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7981 ctttgggccg cctccccgca tcgggaattc gctcaagctt cgaattaatt ctgcagagct
     8041 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8101 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatggg atcaattcac
     8161 catgggaata acttcgtata gcatacatta tacgaagtta tgctgctttt tgcttgtact
     8221 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8281 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8341 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8401 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     8461 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     8521 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     8581 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     8641 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     8701 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     8761 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     8821 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     8881 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     8941 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9001 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     9061 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     9121 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     9181 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     9241 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9301 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9361 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9421 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9481 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9541 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9601 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9661 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9721 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9781 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9841 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9901 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9961 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    10021 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10081 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10141 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10201 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10261 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10321 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10381 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10441 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10501 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10561 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10621 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10681 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10741 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10801 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10861 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10921 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10981 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11041 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    11101 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    11161 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
    11221 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    11281 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    11341 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    11401 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    11461 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    11521 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
    11581 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
    11641 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
    11701 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
    11761 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
    11821 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
    11881 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
    11941 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
    12001 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
    12061 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
    12121 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
    12181 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
    12241 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
    12301 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
    12361 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
    12421 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
    12481 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
    12541 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
    12601 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
    12661 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
    12721 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
    12781 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
    12841 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
    12901 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
    12961 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
    13021 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
    13081 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
    13141 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
    13201 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
    13261 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    13321 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    13381 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    13441 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    13501 ctgccaatta cctagtggtt tcatttactc taaacctgtg attcctctga attattttca
    13561 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtag
//
